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Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces

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Abstract

Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectin-mediated cell adhesion to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures and subsequent binding of ECM glycoproteins. First, we synthesized β-GlcNAc glycosides carrying a linker for controlled coupling onto chemically functionalized surfaces. Then we produced poly-LacNAc structures with defined lengths using human β1,4-galactosyltransferase-1 and β1,3-N-acetylglucosaminyltransferase from Helicobacter pylori. These compounds were also used for kinetic characterization of glycosyltransferases and lectin binding assays. A mixture of poly-LacNAc-structures covalently coupled to functionalized microtiter plates were identified for best binding to our model galectin His6CGL2. We further demonstrate for the first time that these poly-LacNAc surfaces are suitable for further galectin-mediated binding of the ECM glycoproteins laminin and fibronectin. This new technology should facilitate cell adhesion to biofunctionalized surfaces by imitating the natural ECM microenvironment.

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Acknowledgements

The authors thank Prof. Dr. Markus Aebi (ETH Zürich) and Prof. Dr. Eric G. Berger (Zürich University) for providing the CGL2 plasmid and α3GalT plasmid, respectively. L.E. and B.S acknowledge financial support by the DFG Research Training Group 1035 “Biointerface”. Part of this work was supported by a bilateral grant from DAAD-AV ČR project PPP-D7-CZ 26/04-05D/03/44448 (V. K. & L. E.) and by projects MSMT LC06010 and GAAVCR IAA400200503. B.S. thanks the Boehringer Ingelheim Foundation—Travel Allowances for financial support during a stay in Dr. Wakarchuk’s laboratory. Dr. P. Halada (Inst. Microbiol., Prague) is thanked for the MS measurements. The excellent technical assistance (HPLC/ESI-MS and CE) by Dipl.-Ing. Dennis Hirtz (Laboratory for Biomaterials, RWTH Aachen University) is gratefully acknowledged.

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  1. Laboratory for Biomaterials, Institute of Biotechnology and Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, 52074, Aachen, Germany

    Birgit Sauerzapfe, Judith Schmiedel & Lothar Elling

  2. Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20, Prague 4, Czech Republic

    Karel Křenek, Helena Pelantová & Vladimir Křen

  3. Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, K1A 0R6, Canada

    Warren W. Wakarchuk

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  1. Birgit Sauerzapfe
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  2. Karel Křenek
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Sauerzapfe, B., Křenek, K., Schmiedel, J. et al. Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces. Glycoconj J 26, 141–159 (2009). https://doi.org/10.1007/s10719-008-9172-2

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