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Zinc-induced Cell Death in Rice (Oryza Sativa L.) Roots

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Abstract

Cell death in rice roots due to zinc (Zn) toxicity was investigated using inhibitors of signal molecules known to regulate programmed cell death in plants. Zn (5.0– 25.0 mM) induced cell death in a dose- and time-dependent manner. Sodium benzoate, a scavenger of reactive oxygen species (ROS), increased the cell viability under toxic Zn level (25.0 mM), suggesting a role of ROS in Zn-induced cell death. The protective role of rotenone in cell death indicated the involvement of mitochondrial electron transport chain in this Zn-induced ROS generation. Cantharidin and endothall, two serine/threonine phosphatase inhibitors, and sodium orthovanadate (Na3VO4) and phenylarsine oxide (PAO), two protein tyrosine phosphatase inhibitors, blocked Zn-induced root cell death. Conversely, K252-a, a serine/threonine kinase inhibitor, increased Zn-induced cell death. Furthermore, the phosphatidylinositol 3-Kinase (PI-3K) inhibitors, LY 294002 and wortmannin inhibited Zn-induced root cell death. These results suggest that the ROS, protein phosphatase and PI-3K may function in the Zn-induced cellular toxicity in rice roots.

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Abbreviations

MAPK:

mitogen-activated protein kinase

PAO:

phenylarsine oxide

PI-3K:

phosphatidylinositol 3-kinase

ROS:

reactive oxygen species

SB:

sodium benzoate

Na3VO4:

sodium orthovanadate

Zinc:

Zn

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Authors and Affiliations

  1. Department of Life Sciences, National Cheng Kung University, No.1 University Rd, 701, Tainan, Taiwan ROC

    Hsien-Bing Chang, Chung-Wen Lin & Hao-Jen Huang

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  1. Hsien-Bing Chang
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  2. Chung-Wen Lin
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  3. Hao-Jen Huang
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Correspondence to Hao-Jen Huang.

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Chang, HB., Lin, CW. & Huang, HJ. Zinc-induced Cell Death in Rice (Oryza Sativa L.) Roots. Plant Growth Regul 46, 261–266 (2005). https://doi.org/10.1007/s10725-005-0162-0

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