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The Activity and Localization of Lipoxygenases in Arabidopsis thaliana under Cadmium and Copper Stresses

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Abstract

The regulation of lipoxygenases (LOX) activity was studied in Arabidopsis thaliana grown under excess Cd and Cu (0.0, 5.0, 50.0 μM) in solution cultures for 7 days. The LOX activity was determined spectrophotometrically at 234 nm in the leaves of mature plants using linoleic and linolenic acids as substrates, while their cellular localization was assessed by immunogold-labelling. The LOX mainly occurred in the cytoplasm as well as inside the chloroplasts. Two bands of LOX were found in the leaves of A. thaliana, but one more band appeared in plants treated with 50.0 μM Cu. Despite the lower amount of enzymatic protein in plants exposed to heavy metals, the enzyme activity was significantly higher than that in the control; it was especially high at pH 8.0 when linolenic acid was used as a substrate. The role of the redox state of plant cells in modifying the LOX activity was also discussed. The changes in ultra-structure of the leaf parenchyma cells were more evident in A. thaliana plants treated with Cd than those treated with Cu.

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Abbreviations

AA:

scorbate

DAB:

3,3′-diaminobenzidine

GSH:

reduced glutathione

JA:

jasmonic acid

LOX:

lipoxygenases

PAb:

polyclonal antibody

TEM:

transmission electron microscopy

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Authors and Affiliations

  1. Department of Plant Physiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland

    Ewa Skórzyńska-Polit, Maria Drążkiewicz & Zbigniew Krupa

  2. Department of Comparative Anatomy and Anthropology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland

    Bożena Pawlikowska-Pawlęga

  3. Department of Plant Anatomy and Cytology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland

    Ewa Szczuka

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  1. Ewa Skórzyńska-Polit
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  2. Bożena Pawlikowska-Pawlęga
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  3. Ewa Szczuka
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  5. Zbigniew Krupa
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Correspondence to Ewa Skórzyńska-Polit.

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Skórzyńska-Polit, E., Pawlikowska-Pawlęga, B., Szczuka, E. et al. The Activity and Localization of Lipoxygenases in Arabidopsis thaliana under Cadmium and Copper Stresses. Plant Growth Regul 48, 29–39 (2006). https://doi.org/10.1007/s10725-005-4745-6

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