Abstract
Effect of fungal elicitors on biomass and asiaticoside production in multiple shoot cultures of Centella asiatica was studied in a dose- and culture age- dependent manner. Addition of 3 % v/v Trichoderma harzianum culture filtrate (CF) in the growth medium on 10th day of 35 days culture cycle resulted in 9.63 mg g−1dry weight content and 1.15 mg dry weight culture−1 yield of asiaticoside that was 2.53 and 2.35 times higher than in the un-elicited control shoots, respectively. The elicited cultures also registered 1.24 folds more biomass over the control with a growth index (GI) of 7.67, calculated as fresh weight increment over the initial inoculum weight. Elicitation of shoots with mycelial extract (ME) of the fungus Colletotrichum lindemuthianum (1.5 % v/v, added on 0 day) on the other hand, though favored highest biomass accumulation amongst all the elicitation treatments (GI = 16.10) resulted in a decreased asiaticoside content of 1.10 mg g−1 dry weight that was nearly 3.5 and 8.7 times lower than in the control or T. harzianum CF-treated shoots, respectively. Treatments with mycelial extract of Fusarium oxysporum (0.5–1.5 % v/v), in general, proved inhibitory for shoot growth if added on 0 day of the culture cycle with GI = 4.85–8.45 in comparison to 11.11 in the control with a poor asiaticoside yield of only 0.18–0.42 mg dry weight culture−1. Though the shoot biomass accumulation was marginally improved (GI = 5.68–11.94) over the untreated control when F. oxysporum ME (0.5–1.5 % v/v) was added in the medium on the 30th day of culture but the asiaticoside yield (0.18–0.94 mg dry weight culture−1) remained low. The results presented here demonstrate for the first time the potential application of T. harzianum CF in up-regulating the asiaticoside biogenetic pathway in C. asiatica.
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Acknowledgments
The present work is being carried out under a sponsored project grant No.SR/SO/Ps-28/07 of the Department of Science and Technology, New Delhi (India). The authors are also grateful to Director CIMAP, Lucknow and Council of Scientific and Industrial Research, New Delhi for providing the necessary facilities.
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Prasad, A., Mathur, A., Kalra, A. et al. Fungal elicitor-mediated enhancement in growth and asiaticoside content of Centella asiatica L. shoot cultures. Plant Growth Regul 69, 265–273 (2013). https://doi.org/10.1007/s10725-012-9769-0
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DOI: https://doi.org/10.1007/s10725-012-9769-0