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Quantitative proteomics study on Lsi1 in regulation of rice (Oryza sativa L.) cold resistance

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Abstract

Low temperature is one of the major abiotic stresses which severely affects the productivity and the geographical distribution of rice (Oryza sativa). Silicon is considered a broad spectrum alleviator to combat stress in rice plant. Rice root absorbs silicon by a silicon transporter, Low silicon gene 1 (Lsi1). To gain a better understanding of cold stress responses triggered by overexpression of Lsi1 in rice (Oryza sativa L.), we carried out physiological and molecular studies between Lsi1-overexpression Dular (Lsi1-D) and its wild type (WD). Two leaf stage rice seedlings of above mentioned both lines were treated at 15 °C/12 °C (day/night) for 7 days. WD seedling leaves were turned comparatively yellow as compared to Lsi1-D seedlings. Microscopic studies showed significantly more deposition of silicon bodies in epidermal cells of Lsi1-D leaf seedlings in comparison with WD leaves. Lsi1-D leaves comparatively, depicted more SOD, POD and CAT activity, chlorophyll a, b contents in consistency with more silicon concentration. Protein extraction was carried out from whole seedling of both lines and further analyzed by tandem mass tag quantitative proteomics approach with double replicates. Among 393 reproducible proteins, 63 were up-regulated and 39 proteins were down-regulated. The total cold responsive differential proteins were involved in several processes, i.e. photosynthesis, signal transduction, redox homeostasis, hormone metabolism, carbohydrate metabolism, cell wall organization, N-assimilation, protein processing and secondary metabolism. We confirmed up-regulation of key proteins involved in cold-responsive pathway at mRNA level through qPCR such as chlorophyll a–b binding protein 1, peroxidase 2, signaling G-proteins RIC1, aquaporin PIP1.2, 1, 4-alpha-glucan branching enzyme, germin-like protein subfamily 2 member 4 and germin-like protein subfamily 8 member 2. In conclusion, our study provides new insights into cold stress responses in rice seedlings triggered by Lsi1-overexpression defense pathway.

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Abbreviations

Si:

Silicon

WD:

Wild Dular

TLS:

Two leaf stage

Lsi1 :

Low silicon rice 1

Lsi1-D:

Lsi1-overexpressed Dular

NaOH:

Sodium hydroxide

FAA:

Formaldehyde

PBS:

Potassium phosphate buffer

TCA:

Trichloroacetic acid

TMT:

Tandem mass tags

D/N:

Day/night

SD:

Supplementary data

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Acknowledgments

Thanks to Dr. Changxun Fang for providing Transgene rice line (Lsi1 overexpressed Dular) and for his continuous guidance to accomplish this research. This work was supported by the National Natural Science Foundation of China (Nos. 31271670, 31300336) and the National Research Foundation for the Doctoral Program of Higher Education of China (No. 20133515130001).

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Authors and Affiliations

  1. Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China

    Saadia Azeem, Zhong Li, Haiping Zheng, Yasir Arafat, Zhixing Zhang, Xiangmin Lin & Wenxiong Lin

  2. Key Laboratory of Crop Ecology and Molecular Physiology of Fujian Universities, Fujian Agriculture and Forestry University, Fuzhou, 35002, China

    Weiwei Lin, Zhixing Zhang, Xiangmin Lin & Wenxiong Lin

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  1. Saadia Azeem
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  2. Zhong Li
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Correspondence to Wenxiong Lin.

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Azeem, S., Li, Z., Zheng, H. et al. Quantitative proteomics study on Lsi1 in regulation of rice (Oryza sativa L.) cold resistance. Plant Growth Regul 78, 307–323 (2016). https://doi.org/10.1007/s10725-015-0094-2

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