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Luteolin Relieved DSS-Induced Colitis in Mice via HMGB1-TLR-NF-κB Signaling Pathway

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Abstract

The aim of this study was to investigate the effect of luteolin (Lu) on dextran sodium sulfate (DSS)–induced colitis in mice. Mice spleen was weighed. The length of colon was measured. H&E staining was used to observe the pathological changes of colon in mice. Superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and intestine of mice were detected by commercial kits. Serum and intestinal cytokines were detected by ELISA kits. The expression of HMGB1 mRNA was detected by real-time PCR. The expression of HMGB1-TLR-NF-κB pathway was detected by Western blot. The level of HMGB1 was detected by immunohistochemistry. The results showed that Lu significantly increased the colon length/body weight ratio and significantly decreased the spleen weight/body weight ratio. Lu significantly increased serum and intestinal SOD levels and decreased MDA levels. Lu significantly increased serum and intestinal cytokine levels in mice. qPCR and immunohistochemistry results showed that Lu significantly reduced HMGB1 mRNA level and protein level. In addition, Lu significantly reduced the expression of HMGB1-TLR-NF-κB signaling pathway protein of intestine in mice. In conclusion, Lu significantly reduced and alleviated DSS-induced colitis in mice, and the mechanism was related to the regulation of intestinal HMGB1-TLR-NF-κB signaling pathway in mice.

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Funding

This work was funded by the Jiangsu Youth Medical Talents Project (QNRC2016255) and Science Foundation of Bayinguolengmengguzizhizhou People’s Hospital (BHS201913).

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Author notes

  1. Ting Zuo and Yinzi Yue contributed equally to this work.

Authors and Affiliations

  1. Henan University of Chinese Medicine, Zhengzhou, 450046, Henan, China

    Ting Zuo & Huan Li

  2. Department of General Surgery, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, 215009, Jiangsu, China

    Yinzi Yue

  3. Department of General Surgery, Bayinguolengmengguzizhizhou People’s Hospital, Korla, 841000, Xinjiang, China

    Xiaohui Wang

  4. Department of Anorectal Surgery, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, 215009, Jiangsu, China

    Shuai Yan

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  1. Ting Zuo
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  2. Yinzi Yue
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  3. Xiaohui Wang
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  4. Huan Li
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Correspondence to Shuai Yan.

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Zuo, T., Yue, Y., Wang, X. et al. Luteolin Relieved DSS-Induced Colitis in Mice via HMGB1-TLR-NF-κB Signaling Pathway. Inflammation 44, 570–579 (2021). https://doi.org/10.1007/s10753-020-01354-2

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  • DOI: https://doi.org/10.1007/s10753-020-01354-2

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