Skip to main content

Advertisement

SpringerLink
Log in

Chemical characterization and anti-arthritic appraisal of Monotheca buxifolia methanolic extract in Complete Freund’s Adjuvant-induced arthritis in Wistar rats

  • Original Article
  • Published:
Inflammopharmacology Aims and scope Submit manuscript

Abstract

The current study was designed to evaluate the anti-oxidant and anti-arthritic potential of a traditionally used herb, Monotheca buxifolia. The M. buxifolia methanolic extract (MBME) was prepared from the aerial parts of the plant followed by chemical characterization with GC–MS. The anti-oxidant potential of the MBME was demonstrated by DPPH scavenging activity. The effects of MBME on protein denaturation and membrane stabilization were determined by inhibition of egg albumin denaturation and RBC membrane stabilization assays, respectively. The in vivo anti-arthritic potential of the MBME at 50, 100, and 150 mg/kg/day was evaluated in Complete Freund’s Adjuvant-induced polyarthritis in Wistar rats treated for 21 days. Phytochemicals, such as linolenic acid methyl ester, n-hexadecanoic acid, vitamin E, α-amyrin, and β-amyrin were detected in the GC–MS analysis. The plant extract exhibited a 55.20 ± 0.69% scavenging of free radicals at 100 µg/ml concentration. It significantly (p < 0.05) stabilized human RBC membrane (65.06 ± 0.22%) and inhibited protein denaturation (70.53 ± 0.34%) at 100 mg/ml concentration. The diclofenac sodium (DS) and MBME at 150,100, and 50 mg/kg reduced the paw edema, restored the body weight, and altered blood parameters including CRP. The MBME significantly reduced the MDA and increased the SOD, CAT, and GSH levels in liver tissue homogenate in treated rats. The serum concentration of TNF-α and PGE2 was remarkably (p < 0.01–< 0.0001) restored by the DS and MBME dose dependently. The histopathological study showed that MBME 150 mg/kg commendably restored the ankle joint inflammation, bone erosion, and cartilage damage in polyarthritic rats. It was concluded that the anti-oxidant, anti-inflammatory and anti-arthritic effects of MBME might be attributed to phenols, flavonoids, triterpenoids, vitamin E, phytol, and other fatty acids. This study showed the anti-arthritic potential of Monotheca buxifolia and thus validates its traditional claim.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

CAT:

Catalase

GSH:

Reduced glutathione

MDA:

Malondialdehyde

PGE2:

Prostaglandin

SOD:

Superoxide dismutase

TNF-α:

Tumor necrosis factor

References

  • Ahmad S et al (2019) Antimicrobial, anti-oxidant and cytotoxic potential of aerial parts of monotheca buxifolia. J Math Fundam Sci 51:138–151

    Article  CAS  Google Scholar 

  • Akhtar MF, Ashraf M, Anjum AA, Javeed A, Sharif A, Saleem A, Akhtar B (2016) Textile industrial effluent induces mutagenicity and oxidative DNA damage and exploits oxidative stress biomarkers in rats. Environ Toxicol Pharmacol 41:180–186

    Article  CAS  Google Scholar 

  • Akhtar MF et al (2018) Association of textile industry effluent with mutagenicity and its toxic health implications upon acute and sub-chronic exposure. Environ Monit Assess 190:1–13

    Article  CAS  Google Scholar 

  • Bhangale JO, Acharya SR (2016) Anti-Parkinson activity of petroleum ether extract of Ficus religiosa (L.) leaves. Adv Pharmacol Sci 2016:1–9. https://doi.org/10.1155/2016/9436106

    Article  CAS  Google Scholar 

  • Bihani GV, Rojatkar SR, Bodhankar SL (2014) Anti-arthritic activity of methanol extract of Cyathocline purpurea (whole plant) in Freund’s complete adjuvant-induced arthritis in rats. Biomed Aging Pathol 4:197–206

    Article  Google Scholar 

  • Burki S, Burki ZG, Shah ZA, Imran M, Khan M (2018) Phytochemical screening, anti-oxidant and in vivo neuropharmacological effect of Monotheca buxifolia (Falc.) barks extract. Pak J Pharm Sci 31(4):1519–1528

    CAS  PubMed  Google Scholar 

  • Chandra S, Chatterjee P, Dey P, Bhattacharya S (2012) Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of protein. Asian Pac J Trop Biomed 2:178–180

    Article  Google Scholar 

  • Comar JF et al (2013) Oxidative state of the liver of rats with adjuvant-induced arthritis. Free Radic Biol Med 58:144–153

    Article  CAS  Google Scholar 

  • De P, Sarkar S, Mukhophadhyay MJ (2017) Study the anti-oxidant and In vitro Anti-inflammatory activity by membrane stabilization method of Amaranthus gangeticus leaf extract. J Pharmacogn Phytochem 6:103–105

    CAS  Google Scholar 

  • Din BU et al (2018) Antimalarial potential of leaves crude extract of Monotheca buxifolia. Int J Biosci 12:27–34

    Article  CAS  Google Scholar 

  • El-Din SM, Alagawany N (2019) Phytochemical constituents and anticoagulation property of marine algae gelidium crinale, sargassum hornschuchii and ulva linza. Thalassas Int J Marine Sci 35:381–397

    Article  Google Scholar 

  • Fan R, Pan T, Zhu A-L, Zhang M-H (2017) Anti-inflammatory and anti-arthritic properties of naringenin via attenuation of NF-κB and activation of the heme oxygenase (HO)-1/related factor 2 pathway. Pharmacol Rep 69:1021–1029

    Article  CAS  Google Scholar 

  • Gabay O et al (2010) Stigmasterol: a phytosterol with potential anti-osteoarthritic properties. Osteoarthr Cartil 18:106–116

    Article  CAS  Google Scholar 

  • Hasan UH (2018) anti-arthritic efficacy of clematis orientalis. Bangladesh J Pharmacol 13:142–148

    Article  Google Scholar 

  • Hira S, Saleem U, Anwar F, Raza Z, Rehman AU, Ahmad B (2020) In silico study and pharmacological evaluation of eplerinone as an anti-Alzheimer’s drug in STZ-induced Alzheimer’s disease model. ACS Omega 5:13973–13983

    Article  CAS  Google Scholar 

  • Hugar AL, Kanjikar AP, Londonkar RL (2017) Bioactive compounds investigation from methanol bark extract of pterocarpus marsupium Using GC-MS analysis. Int J Pharm Qual Assur 8:104–110

    Google Scholar 

  • Jan S, Khan MR (2016) Protective effects of Monotheca buxifolia fruit on renal toxicity induced by CCl 4 in rats. BMC Complement Altern Med 16:289

    Article  Google Scholar 

  • Javed S, Oise IE, Nahar L, Ismail FM, Mahmood Z, Sarker SD (2016) Isolation, identification and antiproliferative activity of triterpenes from the genus Monotheca A. DC Rec Nat Prod 10:782–787

    CAS  Google Scholar 

  • Kaur N, Chaudhary J, Jain A, Kishore L (2011) Stigmasterol: a comprehensive review. Int J Pharm Sci Res 2:2259

    CAS  Google Scholar 

  • Khan N, Ahmed M, Shaukat SS, Wahab M, Siddiqui MF (2011) Structure, diversity, and regeneration potential of Monotheca buxifolia (Falc.) A. DC. dominated forests of Lower Dir District. Pak Front Agric China 5:106–121

    Article  Google Scholar 

  • Melo CM, Morais TC, Tomé AR, Brito GAC, Chaves MH, Rao VS, Santos FA (2011) Anti-inflammatory effect of α, β-amyrin, a triterpene from Protium heptaphyllum, on cerulein-induced acute pancreatitis in mice. Inflamm Res 60:673–681

    Article  CAS  Google Scholar 

  • Narendhirakannan R, Limmy T (2012) Anti-inflammatory and anti-oxidant properties of Sida rhombifolia stems and roots in adjuvant induced arthritic rats. Immunopharmcol Immunotoxicol 34:326–336

    Article  CAS  Google Scholar 

  • Pepys MB, Hirschfield GM (2003) C-reactive protein: a critical update. J Clin Investig 111:1805–1812

    Article  CAS  Google Scholar 

  • Ruan Q, Zhao C, Ye Z, Ruan J, Xie Q, Xie W (2015) Effect and possible mechanism of monocyte-derived VEGF on monocyte–endothelial cellular adhesion after electrical burns. Burns 41:825–832

    Article  Google Scholar 

  • Saeed N, Khan MR, Shabbir M (2012) anti-oxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 12:221

    Article  CAS  Google Scholar 

  • Saikarthik J, Ilango S, Vijayakumar J, Vijayaraghavan R (2017) Phytochemical analysis of methanolic extract of seeds of Mucuna pruriens by gas chromatography mass spectrometry. Int J Pharm Sci Res 8:2916–2921

    CAS  Google Scholar 

  • Saleem A, Saleem M, Akhtar MF, Sharif A, Javaid Z, Sohail K (2019) vitro and in vivo anti-arthritic evaluation of Polystichum braunii to validate its folkloric claim. Pak J Pharm Sci 32(3):1167–1173

    CAS  PubMed  Google Scholar 

  • Saleem A, Saleem M, Akhtar MF (2020) anti-oxidant, anti-inflammatory and anti-arthritic potential of Moringa oleifera Lam: an ethnomedicinal plant of Moringaceae family. S Afr J Bot 128:246–256

    Article  CAS  Google Scholar 

  • Saleem A, Saleem M, Akhtar MF, Shahzad M, Jahan S (2020a) Moringa rivae leaf extracts attenuate Complete Freund’s adjuvant-induced arthritis in Wistar rats via modulation of inflammatory and oxidative stress biomarkers. Inflammopharmacology 28:139–151

    Article  CAS  Google Scholar 

  • Saleem A, Saleem M, Akhtar MF, Shahzad M, Jahan S (2020b) Polystichum braunii extracts inhibit Complete Freund’s adjuvant-induced arthritis via upregulation of I-κB, IL-4, and IL-10, downregulation of COX-2, PGE2, IL-1β, IL-6, NF-κB, and TNF-α, and subsiding oxidative stress. Inflammopharmacology 28:1–16

    Article  Google Scholar 

  • Shabbir A, Batool SA, Basheer MI, Shahzad M, Sultana K, Tareen RB, Iqbal J (2018) Ziziphora clinopodioides ameliorated rheumatoid arthritis and inflammatory paw edema in different models of acute and chronic inflammation. Biomed Pharmacother 97:1710–1721

    Article  Google Scholar 

  • Ullah I, Khan JA, Adhikari A, Khan A, Hannan PA, Wadood A, Farooq U (2016) Bioassay-guided isolation of new urease inhibitory constituents from Monotheca buxifolia (Falc.) fruit and their molecular docking studies. Rec Nat Prod 10:744–754

    CAS  Google Scholar 

  • Waterborg JH (2009) The Lowry method for protein quantitation. In: Walker JM (ed) The protein protocols handbook. Springer, Totowa, pp 7–10

    Chapter  Google Scholar 

  • Zhang Q et al (2020) Iridoid glycosides from Morinda officinalis How. exert anti-inflammatory and anti-arthritic effects through inactivating MAPK and NF-κB signaling pathways. BMC Complement Med Ther 20:1–14

    Article  Google Scholar 

  • Zhang Z, Chinnathambi A, Alharbi SA, Bai L (2020) Copper oxide nanoparticles from Rabdosia rubescens attenuates the complete Freund’s adjuvant (CFA) induced rheumatoid arthritis in rats via suppressing the inflammatory proteins COX-2/PGE2. Arab J Chem 13:5639–5650

    Article  CAS  Google Scholar 

Download references

Acknowledgement

We are grateful to Riphah International University, Lahore campus, Pakistan, for providing chemicals and technical facilities. This work was supported by King Saud University, Deanship of Scientific Research, College of Science Research Center.

Author information

Authors and Affiliations

  1. Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore campus, Lahore, Pakistan

    Muhammad Furqan Akhtar & Khushdil Khan

  2. Department of Pharmacology, Government College University Faisalabad, Faisalabad, Pakistan

    Ammara Saleem

  3. Laboratory of Stem Cells Research and Biomedical Engineering for Novel Biofunctional, and Pharmaceutical Nanomaterials, Faculty of Dentistry, Prince Philip Dental Hospital, The University of Hong Kong, Hong Kong, 999077, China

    Mirza Muhammad Faran Ashraf Baig

  4. Department of Zoology, Government College University Faisalabad, Faisalabad, Pakistan

    Azhar Rasul

  5. Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia

    Mohamed M. Abdel-Daim

  6. Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

    Mohamed M. Abdel-Daim

Authors
  1. Muhammad Furqan Akhtar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Khushdil Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ammara Saleem
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mirza Muhammad Faran Ashraf Baig
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Azhar Rasul
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mohamed M. Abdel-Daim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muhammad Furqan Akhtar.

Ethics declarations

Conflict of interest

Authors have declared no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Akhtar, M.F., Khan, K., Saleem, A. et al. Chemical characterization and anti-arthritic appraisal of Monotheca buxifolia methanolic extract in Complete Freund’s Adjuvant-induced arthritis in Wistar rats. Inflammopharmacol 29, 393–408 (2021). https://doi.org/10.1007/s10787-020-00783-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10787-020-00783-7

Keywords

Search

Navigation