Skip to main content
SpringerLink
Log in

Effects of type and state of co-culture cells on in-vitro development of porcine oocytes matured and fertilized in vitro

  • Assisted Reproduction
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose: The present study was to investigate the impact of type and state of co-culture cells on developmental competence of porcine oocytes matured and fertilized in vitro.

Methods: Porcine zygotes were co-cultured with granulosa cells (GCs) (Group 1) or porcine oviductal epithelial cells (pOECs) at follicular stage (Group 2), ovulation stage (Group 3) or corpus luteum (CL) stage (Group 4) or cultured in a medium without co-culture cells (control group).

Results: The proportion of oocytes developed to 2-cell stage embryos in Group 2 was similar to that in control group, but significantly (p < 0.05) lower than that in Groups 1, 3 and 4. The proportions of oocytes developed to ≥4-cell stage embryos in Groups 3 and 4 were significantly (p < 0.05) higher than that in Groups 1 and 2. At 144 h after insemination, 12.0, 14.8 and 20.0% of oocytes developed to blastocysts in Groups 1, 3 and 4, respectively. However, no embryos in control group developed beyond 4-cell stage and no embryos in Group 2 developed to blastocyst stage.

Conclusion: As compared with GCs and pOECs at follicular stage, the pOECs at ovulation and CL stages had a better competence to support porcine embryo development under in vitro conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vanroose G, Van Soom A, de Kruif A: From co-culture to defined medium: State of the art and practical considerations. Reprod Domest Anim 2001;36(1):25–28

    CAS  PubMed  Google Scholar 

  2. Thompson JG: In vitro and embryo metabolism of cattle and sheep embryos—a decade of achievement. Anim Reprod Sci 2000;60–61:263–275

    PubMed  Google Scholar 

  3. Kikuchi K, Naito K, Daen FP, Izaike Y, Toyoda Y: Histone H1 kinase activity during in vitro fertilization of pig follicular oocytes matured in vitro. Theriogenology 1995;43:523–532

    CAS  Google Scholar 

  4. Rath D, Freitag K, Niemann H, Sieg B: Continuous control of porcine embryo development in vitro. Theriogenology 1998;49:215(abstract)

    Google Scholar 

  5. Rath D: Experiments to improve in vitro fertilization techniques for in vivo matured porcine oocytes. Theriogenology 1992;37:885–896

    Google Scholar 

  6. Koo DB, Kim NH, Lee HT, Chung KS: Effects of fetal calf serum, amino acids, vitamins and insulin on blastocel formation and hatching of in vivo and IVM/IVF-derived porcine embryos developing in vitro. Theriogenology 1997;48:791–802

    CAS  Google Scholar 

  7. Rath D, Niemann H, Torres CRL: In vitro development to blastocysts of early porcine embryos produced in vivo or in vitro. Theriogenology 1995;43:913–926

    Google Scholar 

  8. Dobrinsky, Johnson LA, Rath D: Development of a culture medium (BECM-3) for porcine embryos: Effects of bovine serum albumin and fetal bovine serum on embryo development. Biol Reprod 1996;55:1069–1074

    CAS  PubMed  Google Scholar 

  9. Pushpakumara PG, Robinson RS, Demmers KJ, Mann GE, Sinclair KD, Webb R, Wathes DC: Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy. Reproduction 2002;123(6):859–868

    CAS  PubMed  Google Scholar 

  10. Smith S, Schmidt M, Purwantara B, Greve T: Oviduct epithelial-cell co-culture of early porcine embryos. Acta Vet Scan 1992;33:349–355

    CAS  Google Scholar 

  11. Torres CRL, Rath D: In vitro culture of porcine embryos to the blastocyst stage after in vivo and in vitro fertilization. Theriogenology 1992;37:283 (abstract)

    Google Scholar 

  12. Kidson A, Schoevers E, Langendijk P, Verheijden J, Colenbrander B, Bevers M: The effect of oviductal epithelial cell co-culture during in vitro maturation on sow oocyte morphology, fertilization and embryo development. Theriogenology 2003; 59(9):1889–1903

    PubMed  Google Scholar 

  13. Gilbert CL, Cripps PJ, Wathes DC: The effect of oxytocin on the pattern of electromyographic activity in the oviduct and uterus of the ewe around oestrus. Reprod Fertil Dev 1992;4:193–203

    CAS  PubMed  Google Scholar 

  14. Qian Y, Shi WQ, Ding JT, Sha JH, Fan BQ: Predictive value of the area of expanded cumulus mass on development of porcine oocytes matured and fertilized in vitro. J Reprod Dev 2003;49(2):167–174

    PubMed  Google Scholar 

  15. Gardner DK, Schoolcraft WB: Human embryo viability: What determines developmental potential, and can it be assessed? J Assist Reprod Genet 1998;15:455–458

    CAS  PubMed  Google Scholar 

  16. Pope WF, Xia S, Broermann DM, Nephew KP: Causes and consequences of early embryonic diversity in pigs. J Reprod Fertil 1990;40(suppl):251–260

    CAS  Google Scholar 

  17. Telford NA, Waeson AJ, Schultz GA: Transition from maternal to embryonic control in early mammalian development: a comparison of several species. Mol Reprod Dev 1990;26:90–100

    CAS  PubMed  Google Scholar 

  18. Kane MT, Carney EW, Ellington JE: The role of nutrients, peptide growth factors and co-culture cells in development of preimplantation embryos in vitro. Theriogenology 1992;38:297–313

    CAS  Google Scholar 

  19. White KL, Hehnke K, Rickords LF, Southern LL, Thompson DL, Wood TC: Early embryonic development in vitro by co-culture with oviductal epithelial cells in pigs. Biol Reprod 1989;41:425–430

    CAS  PubMed  Google Scholar 

  20. Bureau M, Bailey JL, Sirard MA: Influence of oviductal cells and conditioned medium on porcine gametes. Zygote 2000;8:139–144

    CAS  PubMed  Google Scholar 

  21. Watson AJ, Watson PH, Warnes D, Walker SK, Armstrong DT, Seamark RF: Preimplantaion development of in-vitro matured and in-vitro fertilized ovine zygotes: Comparison oviduct epithelial cell monolayers and culture under low oxygen atmosphere. Biol Reprod 1994;50:715–724

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Jiangsu Province Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 210029, P. R. China

    Yun Qian & Jia Hao Sha

  2. Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P. R. China

    Wei Qun Shi & Bi Qin Fan

  3. College of Animal Science and Veterinary Medicine, Yangzhou University, Yangzhou, 225009, P. R. China

    Jia Tong Ding

  4. Human Clinical Reproductive Medicine Center, Department of Obstetrics & Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, P. R. China

    Yun Qian & Jia Yin Liu

Authors
  1. Yun Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Qun Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jia Tong Ding
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jia Yin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jia Hao Sha
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bi Qin Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jia Yin Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Qian, Y., Shi, W.Q., Ding, J.T. et al. Effects of type and state of co-culture cells on in-vitro development of porcine oocytes matured and fertilized in vitro. J Assist Reprod Genet 22, 233–238 (2005). https://doi.org/10.1007/s10815-005-5145-6

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-005-5145-6

Keywords

Search

Navigation