Skip to main content
SpringerLink
Log in

Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging

  • Assisted Reproduction Technologies
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

In the current study, our aim was to demonstrate that EmbryoScope incubation conditions is comparable to standard laboratory incubation circumstances by comparing embryo quality, development and ongoing pregnancy rates between the EmbryoScope (ES) and a standard incubator (SI). We analyzed 478 embryos from 60 couples undergoing oocyte donation were included in the study.

Methods

All embryos retrieved from a patient were randomly distributed in the ES or SI. We calculated blastocyst development rate, blastocyst viability and ongoing pregnancy rate for embryo transfers from ES, SI and mixed (one embryo from the ES and one from the SI). Statistical analysis was conducted by Chi square tests, considering p < 0.05 significant.

Results

No significant differences were found between the ES and SI from all the parameters evaluated.

Conclusions

Thus we concluded that time-lapse monitoring in the EmbryoScope does not impair embryo quality while allowing for morphological, spatial and temporal analysis of embryo development.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Mio Y. Morphological analysis of human embryonic development using time-lapse cinematography. J Mamm Ova Res. 2006;23:27–35.

    Article  Google Scholar 

  2. Ebner T, Yaman C, Moser M, Sommergruber M, Feichtinger O, Tews G. Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod. 2000;15(2):427–30.

    Article  PubMed  CAS  Google Scholar 

  3. Scott LA, Smith S. The successful use of pronuclear embryo transfers the day following oocyte retrieval. Hum Reprod. 1998;13(4):1003–13.

    Article  PubMed  CAS  Google Scholar 

  4. Van Montfoort AP, Dumoulin JC, Kesters AD, Evers JL. Early cleavage is a valuable addition to existing embryo selection parameters: a study using single embryo transfers. Hum Reprod. 2004;19(9):2103–8.

    Article  PubMed  Google Scholar 

  5. Payne D, Flaherty SP, Barry MF, Matthews CD. Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod. 1997;12(3):532–41.

    Article  PubMed  CAS  Google Scholar 

  6. Nakahara T, Iwase A, Goto M, Harata T, Suzuki M, Ienaga M, et al. Evaluation of the safety of time-lapse observations for human embryos. J Assist Reprod Genet. 2010;27:93–6.

    Article  PubMed  Google Scholar 

  7. Yamagata K, Suetsugu R, Wakayama T. Long-term, six-dimensional live-cell imaging for the mouse preimplantation embryo that does not affect full-term development. J Reprod Dev. 2009;55:343–50.

    Article  PubMed  Google Scholar 

  8. Budak E, Garrido N, Reis Soares S, Melo MAB, Meseguer M, Pellicer A, et al. Improvements achieved in oocyte donation program over10-year period: sequential icrease in implantation and pregnancy rates and decrease in high-order multiple pregnancies. Fertil Steril. 2007;88(2):342–9.

    Article  PubMed  Google Scholar 

  9. Cobo A, Meseguer M, Remohi J, Pellicer A. Use of cry-banked oocytes in an ovum donation program: a prospective, controlled, clinical trial. Hum Reprod. 2010;25:2239–46.

    Article  PubMed  Google Scholar 

  10. Melo M, Busso CE, Bellver J, Alama P, Garrido N, Meseguer M, et al. GnRH agonist versus recombinant hCG in an oocyte donation program: a randomized, prospective, controlled, asesor-blind study. RBM Online. 2009;19(4):486–92.

    PubMed  CAS  Google Scholar 

  11. Gardner DK, Schollcarft WB. In vitro culture of human blastocysts. In: Jansen R, Mortimer D, editors. Towards reproductive certainty: fertility and genetics beyond 1999: the plenay proceedings on the 11th World Congress on In Vitro Fertilization & Human Reproductive Genetics. Pearl River: Parthenon; 1999. p. 378–88.

    Google Scholar 

  12. Ugajin T, Terada Y, Hasegawa H, Velayo CL, Nabeshima H, Yaegashi N. Aberrant behavior of mouse embryo development after blastomere biopsy as observed through time-lapse cinematography. Fertil Steril. 2010;93(8):2723–8.

    Article  PubMed  Google Scholar 

  13. Mio Y, Maeda K. Time-lapse cinematography of dynamic changes occurring during in vitro development of human embryos. Am J Obstet Gynecol. 2008;199:660.e1–5.

    Article  Google Scholar 

  14. Wong CC, Loewke KE, Bossert NL, Behr B, De Jonge CJ, Baer TM, et al. Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat Biotechnol. 2010;28(10):1115–21.

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

Authors want to thank Sara Fortuño, Noemí Galindo, María Roldán, Niels Ramsing and Karen Marie Hilligsøe for their clinical and technical support in the management of this paper

Author information

Authors and Affiliations

  1. Instituto Valenciano de Infertilidad, Alicante, Spain

    María Cruz, Blanca Gadea, Nicolás Garrido, Mar Martínez, Inma Pérez-Cano, Manuel Muñoz & Marcos Meseguer

  2. Instituto Universitario IVI Valencia, Universidad de Valencia (IUIVI), Valencia, Spain

    Nicolás Garrido & Marcos Meseguer

  3. Unisense Fertilitech, Aarhus, Denmark

    Kamilla Søe Pedersen

  4. Instituto Universitario IVI, Plaza de la Policía Local 3, Valencia, 46015, Spain

    Marcos Meseguer

Authors
  1. María Cruz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Blanca Gadea
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nicolás Garrido
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kamilla Søe Pedersen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mar Martínez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Inma Pérez-Cano
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Manuel Muñoz
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Marcos Meseguer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to María Cruz.

Additional information

Capsule

EmbryScope incubation conditions and time-lapse image acquisition does not affect embryo quality and reproductive outcome. We evaluated the effects of dynamic assessment of embryo quality, a tool that would give more information from the individual embryo in order to improve selection.

This work was presented in the 66th Annual Meeting of the American Society for the Reproductive Medicine in Denver (Colorado, United States)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cruz, M., Gadea, B., Garrido, N. et al. Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging. J Assist Reprod Genet 28, 569–573 (2011). https://doi.org/10.1007/s10815-011-9549-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-011-9549-1

Keywords

Search

Navigation