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Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation.

Methods

Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed by a shotgun proteomic approach (2D nanoUPLC-ESI-MSE). Differentially expressed proteins were used for a functional enrichment study.

Results

Two hundred and fifty-seven proteins were identified or quantified in sperm from the Low and High sperm DNA fragmentation groups. Of these, seventy-one proteins were exclusively or overexpressed in the Low group, whereas twenty-three proteins were exclusively or overexpressed in the High group. One hundred and sixty-three proteins were conserved between these groups. We also functionally related the differentially expressed proteins in viable spermatozoa from the groups. Processes such as triacylglycerol metabolism, energy production, protein folding, response to unfolded proteins, and cellular detoxification were found to be altered in these cells.

Conclusions

Sperm DNA fragmentation is associated with differential protein expression in viable spermatozoa. These proteins may potentially be used as biomarkers for sperm DNA integrity.

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Acknowledgements

The authors wish to thank Fleury/Finep for research funding and financial support. Ms. Intasqui was recipient of a scholarship from the Fundação de Amparo à Pesquisa no Estado de Sao Paulo (FAPESP process number 2011/00385-4).

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Surgery, Division of Urology, Human Reproduction Section, Sao Paulo Federal University – Sao Paulo Hospital, R. Embau, 231, 04039-060, Sao Paulo, Brazil

    Paula Intasqui, Mariana Camargo, Paula T. Del Giudice, Deborah M. Spaine, Agnaldo P. Cedenho & Ricardo P. Bertolla

  2. Fleury Group, Av. Gal. Valdomiro de Lima, 508, 04344-070, Sao Paulo, Brazil

    Valdemir M. Carvalho & Karina H. M. Cardozo

  3. Sao Paulo Federal University, R Embau, 231, 04039-060, Sao Paulo, SP, Brazil

    Paula Intasqui & Ricardo P. Bertolla

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  1. Paula Intasqui
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  2. Mariana Camargo
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  3. Paula T. Del Giudice
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  4. Deborah M. Spaine
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  5. Valdemir M. Carvalho
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  6. Karina H. M. Cardozo
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  7. Agnaldo P. Cedenho
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  8. Ricardo P. Bertolla
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Correspondence to Paula Intasqui.

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Capsule Sperm DNA fragmentation is associated with differential protein expression and biological functions in viable sperm. These proteins may be potentially used as biomarkers for sperm DNA integrity.

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Intasqui, P., Camargo, M., Del Giudice, P.T. et al. Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation. J Assist Reprod Genet 30, 1187–1202 (2013). https://doi.org/10.1007/s10815-013-0054-6

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  • DOI: https://doi.org/10.1007/s10815-013-0054-6

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