Abstract
This work consists on the evaluation of the in vitro performance of Ti6Al4V samples PLD (pulsed laser deposition) coated with hydroxyapatite, both pure and mixed with a CaO–P2O5 glass. Previous studies on immersion of PLD coatings in SBF, showed that the immersion apatite films did not present the usual cauliflower morphology but replicated the original columnar structure and exhibited good bioactivity. However, the influence of glass associated to hydroxyapatite concerning adhesion, proliferation and morphology of MG63 cells on the films surface was unclear. In this study, the performance of these PLD coated samples was evaluated, not only following the physical–chemical transformations resulting from the SBF immersion, but also evaluating the cytocompatibility in contact with osteoblast-like MG63 cells. SEM and AFM confirmed that the bioactive ceramic PLD films reproduce the substrate’s surface topography and that the films presented good adherence and uniform surface roughness. Physical–chemical phenomena occurring during immersion in SBF did not modify the original columnar structure. In contact with MG63 cells, coated samples exhibited very good acceptance and cytocompatibility when compared to control. The glass mixed with hydroxyapatite induced higher cellular proliferation. Cells grown on these samples presented many filipodia and granular structures, typical features of osteoblasts.
Similar content being viewed by others
References
L. L. HENCH, J. Am. Ceram. Soc. 74 (1991) 1487
L. L. HENCH, Biomaterials 19 (1998) 1419
S. H. PARK, A. LLINÁS, V. K. GOEL and J. C. KELLER, in “The Biomedical Engineering Handbook”, Edited by: J. D. BRONZINO (CRC Press, LLC, Boca Raton, 2000)
L. L. HENCH, in “7th International Symposium on Ceramics in Medicine”, Edited by: Ö. H. A. A. Y. URPO (Butterworth-Heinemann Ltd Oxford, England, 1994) p. 3
T. KOKUBO, Biomaterials 12 (1991) 155
H. AOKI, in “Science and Medical Applications of Hydroxyapatite” (JAAS Takayan Press. Ishiyaku euroAmerica, Inc., Tokyo, 1991) p. 6
V. P. ORLOVSKII, V. S. KOMLEV and S. M. BARINOV, Inorg. Mater. 38 (2002) 973
S. M. KENNY and M. BUGGY, J. Mater. Sci. Mat. Med. 14 (2003) 923
J. D. SANTOS, J. C. KNOWLES, R. L. REIS, F. J. MONTEIRO and G. W. HASTINGS, Biomaterials 15 (1994) 5
J. D. SANTOS, R. L. REIS, F. J. MONTEIRO, J. C. KNOWLES and G. W. HASTINGS, J. Mat. Sci. Mat. Med. 6 (1995) 348
M. P. FERRAZ, M. H. FERNANDES, J. D. SANTOS and F. J. Monteiro, J. Mater. Sci.: Mater. Med. 12 (2001) 629
M. P. FERRAZ, F. J. MONTEIRO, D. GIÃO, B. LÉON, P. GONZALEZ, S. LISTE, J. SERRA, J. ÁRIAS and M. PEREZ-AMOR, Key Eng. Mat. 254–256 (2004) 347
A. E. PORTER, L. W. HOBBS, V. BENEZRA ROSEN, M. SPECTOR, Biomaterials 23 (2002) 725
H.-K. KIM, J.-W. JANG and C.-H. LEE, J. Mater. Sci.: Mater. Med. 15 (2004) 825
Y. HARADA, J.-T. WANG, V. A. DOPPALAPUDI, A. A. WILLIS, M. JASTY, W. H. HARRIS, M. NAGASE and S.R. GOLDRING, J. Biomed. Mater. Res. 31 (1996) 19
J. L. ARIAS, M. B. MAYOR, J. POU, B. LÉON and M. PÉREZ-AMOR, Appl. Surf. Sci. 154–155 (2000) 434
J. D. SANTOS, R. L. REIS, F. J. MONTEIRO, J. C. KNOWLES, G. W. HASTINGS, J. Mater. Sci.: Mater. Med. 7 (1996) 187
J. L. ARIAS, M. B. MAYOR, J. POU, Y. LENG, B. LÉON and M. PÉREZ-AMOR, Biomaterials 24 (2003) 3403
J. L. ARIAS, M. B. MAYOR, J. POU, B. LÉON and M. PÉREZ-AMOR, Appl. Surf. Sci. 182 (2002) 448
J. L. ARIAS, M. B. MAYOR, J. POU, B. LÉON and M. PÉREZ-AMOR, Vaccum 67 (2002) 653
J. L. ARIAS, F. J. GARCIA-SANZ, M. B. MAYOR, S. CHIUSSI, J. POU, B. LÉON and M. PÉREZ-AMOR, Biomaterials 19 (1998) 883
M. B. MAYOR, J. L. ARIAS, S. CHIUSSI, F. GARCIA, J. POU, B. LÉON and M. PÉREZ-AMOR, Thin Solid Films 317 (1998) 363
T. KOKUBO, H. KUSHITANI, S. SAKKA, T. KITSUKI and T. YAMAMURO, J. Biomed. Mater. Res. 24 (1990) 721
F. J. MONTEIRO, M. P. FERRAZ, D. GIÃO, B. LEON, P. GONZALEZ, S. LISTE, J. SERRA and M. PEREZ-AMOR, in “17th European Conference on Biomaterials”, Edited by: E. S. F. Biomaterials (Barcelona, Spain, 2002) p. P65
S. LOSSDÖRFER, Z. SCHWARTZ, L. WANG, C. H. LOHMANN, J. D. TURNER, M. WIELAND, D. L. COCHRAN and B. D. BOYAN, J. Biomed. Mater. Res 70A (2004) 361
S. R. BANNISTER, C. H. LOHMANN, Y. LIU, V. L. SYLVIA, D. L. COCHRAN, D. D. DEAN, B. D. BOYAN and Z. SCHWARTZ, J. Biomed. Mater. Res. 60 (2002) 164
L. CLERIES, J. M. FERNANDEZ-PRADAS, G. SARDIN and J. L. MORENZA, Biomaterials 19 (1998) 1483
D. DE SANTIS, C. GUERRIERO, P. F. NOCINI, A. UNGERSBOCK, G. RICHARDS, P. GOTTE and U. ARMATO, J. Mater. Sci.: Mater. Med. 7 (1996) 21
C. KNABE, F. KLAR, R. FITZNER, R. J. RADLANSKI and U. GROSS, Biomaterials 23 (2002) 3235
F. GARCIA, J. L. ARIAS, M. B. MAYOR, J. POU, I. REHMAN, J. KNOWLES, S. BEST, B. LÉON, M. PÉREZ-AMOR and W. BONEFIELD, J. Biomed. Mater. Res. (Appl. Biomater) 43 (1998) 69
P. PENG, S. KUMAR, N. H. VOELCKER, E. SZILI, R. S. C. SMART and H. J. GRIESSER, J. Biomed. Mater. Res. 76A (2006) 347
V. BORSARI, G. GIAVARESI, M. FINI, P. TORRICELI, A. SALITO, R. CHIESA, L. CHIUSOLI, A. VOLPERT, L. RIMONDINI and R. GIARDINO, J. Biomed. Mater. Res. Part B: Appl. Biomater. 75B (2005) 359
C. MASSARO, M. A. BAKER, F. COSENTINO, P. A. RAMIRES, S. KLOSE and E. MILELLA, J. Biomed. Mater. Res. 58 (2001) 651
A. BIGI, B. BRACCI, F. CUISINIER, R. ELKAIM, M. FINI, I. MAYER, I. N. MIHAILESCU, G. SOCOL, L. STURBA and P. TORRICELLI, Biomaterials 26 (2005) 2381
H. ZENG and W. R. LACEFIELD, J. Biomed. Mater. Res. 50 (2000) 239
L. CLÈRIES, J. M. FERNANDEZ-PRADAS and J. L. MORENZA, Biomaterials 21 (2000) 1861
M. P. FERRAZ, F. J. MONTEIRO and J. D. SANTOS, J. Biomed. Mater. Res. 45 (1999) 376
P. LI and P. DUCHEYNE, J. Biomed. Mater. Res. 41 (1998) 341
J. WENG, Q. LIU, J. G. WOLKE, X. ZHANG and K. DE GROOT, Biomaterials 18 (1997) 1027
A. C. TAS, Biomaterials 21 (2000) 1429
T. KOKUBO, H.-M. KIM and M. KAWASHITA, Biomaterials 24 (2003) 2161
J.-L. DEWEZ, A. DOREN, Y.-J. SCHNEIDER and P. G. ROUXHET, Biomaterials 20 (1999) 547
K. ANSELME, P. LINEZ, M. BIGERELLE, D. LE MAGUER, A. LE MAGUER, P. HARDOUIN, H. F. HILDEBRAND, A. IOST and J. M. LEROY, Biomaterials 21 (2000) 1567
A. OKUMURA, M. GOTO, T. GOTO, M. YOSHINARI, S. MASUKO, T. KATSUKI and T. TANAKA, Biomaterials 22 (2001) 2263
H. J. KIM, S. H. KIM, M. S. KIM, E. J. LEE, H. G. OH, W. M. OH, S. W. PARK, W. J. KIM, G. J. LEE, N. G. CHOI, J. T. KOH, D. B. DINH, R. R. HARDIN, K. JOHNSON, V. L. SYLVIA, J. P. SCHMITZ and D. D. DEAN, J. Biomed. Mater. Res. 74A (2005) 366
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Oliveira, G.M., Ferraz, M.P., González, P.G. et al. PLD bioactive ceramic films: the influence of CaO–P2O5 glass additions to hydroxyapatite on the proliferation and morphology of osteblastic like-cells. J Mater Sci: Mater Med 19, 1775–1785 (2008). https://doi.org/10.1007/s10856-007-3331-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10856-007-3331-z