Abstract
Magnesium (Mg) alloys have been suggested as biodegradable bone implant materials due to their good intrinsic biocompatibility and great mechanical properties. Although magnesium has attractive properties as an orthopedic implant material, its quick degradation and low bioactivity may lead to the loss of mechanical integrity of the implant during the bone healing process. In this paper, we endeavor to surmount the abovementioned defects using the surface coating technique. We have recently coated AZ91 magnesium implants with merwinite (Ca3MgSi2O8) through the coupling of plasma electrolytic oxidation (PEO) and electrophoretic deposition method. In this work, we are specifically focused on the in vivo examinations of the coated implants in comparison with the uncoated one. For the in vivo experiment, the rod samples, including the uncoated and merwinite/PEO coated implants, were imbedded into the greater trochanter of rabbits. The results of the in vivo animal test indicated an improvement in biodegradability including slower implant weight loss, reduction in Mg ion released from the coated implants in the blood plasma, lesser release of hydrogen bubbles and an improvement in biocompatibility including an increase in the amount of bone formation and ultimately a mild bone inflammation after the surgery according to the histological images. In summary, proper surface treatment of magnesium implants such as silicate bioactive ceramics may improve their biocompatibility under physiological conditions to making them suitable and applicable for future clinical applications.
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References
Zheng Y, Gu X, Witte F. Biodegradable metals. Mater Sci Eng. 2014;77:1–34.
Alvarez K, Nakajima H. Metallic scaffolds for bone regeneration. Materials. 2009;2(3):790–832.
Niinomi M. Recent metallic materials for biomedical applications. Metall Mater Trans A. 2002;33(3):477–86.
Razavi M, Fathi M, Savabi O, Beni BH, Vashaee D, Tayebi L. Surface microstructure and in vitro analysis of nanostructured akermanite (Ca2MgSi2O7) Coating on biodegradable magnesium alloy for biomedical applications. Colloids Surf B. 2014;117:432–40.
Shahini A, Yazdimamaghani M, Walker K, Eastman M, Hatami-Marbini H, Smith B, et al. 3D conductive nanocomposite scaffold for bone tissue engineering. Int J Nanomed. 2014;9:167–81.
Yazdimamaghani M, Vashaee D, Assefa S, Walker K, Madihally S, Köhler G, et al. Hybrid macroporous gelatin/bioactive-glass/nanosilver scaffolds with controlled degradation behavior and antimicrobial activity for bone tissue engineering. J Biomed Nanotechnol. 2014;10(6):911–31.
Rouhani P, Salahinejad E, Kaul R, Vashaee D, Tayebi L. Nanostructured zirconium titanate fibers prepared by particulate sol–gel and cellulose templating techniques. J Alloy Compd. 2013;568:102–5.
Mozafari M, Vashaee D, Tayebi L. Electroconductive nanocomposite scaffolds: a new strategy into tissue engineering and regenerative medicine. In: Ebrahimi F, editor. Nanocomposites—new trends and developments. INTECH; 2012.
Shabafrooz V, Mozafari M, Vashaee D, Tayebi L. Electrospun nanofibers: from filtration membranes to highly specialized tissue engineering scaffolds. J Nanosci Nanotechnol. 2014;14(1):522–34.
Staiger MP, Pietak AM, Huadmai J, Dias G. Magnesium and its alloys as orthopedic biomaterials: a review. Biomaterials. 2006;27(9):1728–34.
Yazdanpanah A, Kamalian R, Moztarzadeh F, Mozafari M, Ravarian R, Tayebi L. Enhancement of fracture toughness in bioactive glass-based nanocomposites with nanocrystalline forsterite as advanced biomaterials for bone tissue engineering applications. Ceram Int. 2012;38(6):5007–14.
Kirkland N, Birbilis N, Staiger M. Assessing the corrosion of biodegradable magnesium implants: a critical review of current methodologies and their limitations. Acta Biomater. 2012;8(3):925–36.
Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L. In vitro study of nanostructured diopside coating on Mg alloy orthopedic implants. Mater Sci Eng C. 2014;41:168–77.
Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L. Improvement of biodegradability, bioactivity, mechanical integrity and cytocompatibility behavior of biodegradable mg based orthopedic implants using nanostructured bredigite (Ca7MgSi4O16) bioceramic coated via ASD/EPD technique. Ann Biomed Eng. 2014;42(12):1–14.
Farraro KF, Kim KE, Woo SL, Flowers JR, McCullough MB. Revolutionizing orthopaedic biomaterials: the potential of biodegradable and bioresorbable magnesium-based materials for functional tissue engineering. J Biomech. 2013;47:1979–86.
Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L. Biodegradable magnesium alloy coated by fluoridated hydroxyapatite using MAO/EPD technique. Surf Eng. 2014;30(8):545–51.
Yazdimamaghani M, Razavi M, Vashaee D, Tayebi L. Development and degradation behavior of magnesium scaffolds coated with polycaprolactone for bone tissue engineering. Mater Lett. 2014;132:106–10.
Li J, Han P, Ji W, Song Y, Zhang S, Chen Y, et al. The in vitro indirect cytotoxicity test and in vivo interface bioactivity evaluation of biodegradable FHA coated Mg–Zn alloys. Mater Sci Eng B. 2011;176(20):1785–8.
Yazdimamaghani M, Razavi M, Vashaee D, Tayebi L. Surface modification of biodegradable porous Mg bone scaffold using polycaprolactone/bioactive glass composite. Mater Sci Eng C. 2015;49:436–44.
Wong HM, Yeung KW, Lam KO, Tam V, Chu PK, Luk KD, et al. A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants. Biomaterials. 2010;31(8):2084–96.
Salahinejad E, Hadianfard M, Macdonald D, Mozafari M, Vashaee D, Tayebi L. Multilayer zirconium titanate thin films prepared by a sol–gel deposition method. Ceram Int. 2012;39:127
Salahinejad E, Hadianfard M, Macdonald D, Mozafari M, Vashaee D, Tayebi L. Zirconium titanate thin film prepared by an aqueous particulate sol–gel spin coating process using carboxymethyl cellulose as dispersant. Mater Lett. 2012;88:5–8.
Yazdimamaghani M, Razavi M, Vashaee D, Tayebi L. Microstructural and mechanical study of PCL coated Mg scaffolds. Surf Eng. 2014;. doi:10.1179/1743294414Y.0000000307.
Hornberger H, Virtanen S, Boccaccini A. Biomedical coatings on magnesium alloys—a review. Acta Biomater. 2012;8(7):2442–55.
Wu C, Chang J, Xiao Y. Silicate—based bioactive ceramics for bone regeneration application. In: Advanced bioactive inorganic materials for bone regeneration and drug delivery. Taylor & Francis Group, LLC, CRC Press; 2008. p. 25–46. http://books.google.com/books?hl=en&lr=&id=itQOjXSEEp0C&oi=fnd&pg=PA25&dq=Silicate%E2%80%94based+bioactive+ceramics+for+bone+regeneration+application&ots=aw2k0vnFZj&sig=BMo3FxXqeXbaBSJhtenJiJ6pHnA#v=onepage&q=Silicate%E2%80%94based%20bioactive%20ceramics%20for%20bone%20regeneration%20application&f=false
Larsen E, Foshag W. Merwinite, a new calcium magnesium orthosilicate from Crestmore, California. Am Mineral. 1921;6:143–8.
Ou J, Kang Y, Huang Z, Chen X, Wu J, Xiao R, et al. Preparation and in vitro bioactivity of novel merwinite ceramic. Biomed Mater. 2008;3(1):015015.
Hafezi-Ardakani M, Moztarzadeh F, Rabiee M, Talebi AR. Synthesis and characterization of nanocrystalline merwinite (Ca3Mg(SiO4)2) via sol–gel method. Ceram Int. 2011;37(1):175–80.
Razavi M, Fathi M, Savabi O, Hashemi Beni B, Vashaee D, Tayebi L. Nanostructured merwinite bioceramic coating on Mg alloy deposited by electrophoretic deposition. Ceram Int. 2014;40:9473–84.
Witte F, Kaese V, Haferkamp H, Switzer E, Meyer-Lindenberg A, Wirth C, et al. In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials. 2005;26(17):3557–63.
Xu L, Pan F, Yu G, Yang L, Zhang E, Yang K. In vitro and in vivo evaluation of the surface bioactivity of a calcium phosphate coated magnesium alloy. Biomaterials. 2009;30(8):1512–23.
Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L. Biodegradation, bioactivity and in vivo biocompatibility analysis of plasma electrolytic oxidized (PEO) biodegradable Mg implants. Phys Sci Int J. 2014;4(5):708–22.
Song Y, Zhang S, Li J, Zhao C, Zhang X. Electrodeposition of Ca–P coatings on biodegradable Mg alloy: in vitro biomineralization behavior. Acta Biomater. 2010;6(5):1736–42.
Razavi M, Fathi M, Savabi O, Vashaee D, Tayebi L. Micro-arc oxidation and electrophoretic deposition of nano-grain merwinite (Ca3MgSi2O8) surface coating on magnesium alloy as biodegradable metallic implant. Surf Interface Anal. 2014;. doi:10.1002/sia.5465.
Rettig R, Virtanen S. Composition of corrosion layers on a magnesium rare-earth alloy in simulated body fluids. J Biomed Mater Res Part A. 2009;88(2):359–69.
Acknowledgments
The authors are thankful for the contributions of Isfahan University of Technology, Torabinejad Dental Research Center, Oklahoma Center for Advancement of Science and Technology (Grant No. AR131-054 8161), AFOSR (Grant No. FA9550-10-1-0010) and the National Science Foundation (NSF, Grant No. 0933763).
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Razavi, M., Fathi, M., Savabi, O. et al. In vivo biocompatibility of Mg implants surface modified by nanostructured merwinite/PEO. J Mater Sci: Mater Med 26, 184 (2015). https://doi.org/10.1007/s10856-015-5514-3
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DOI: https://doi.org/10.1007/s10856-015-5514-3