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Magnesium ions enhance infiltration of osteoblasts in scaffolds via increasing cell motility

  • Biomaterials Synthesis and Characterization
  • Original Research
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Abstract

Magnesium (Mg) ions are the most abundant intracellular divalent cations and play a pivotal role in numerous cellular processes. Biodegradable Mg-containing materials, including scaffolds, are promising candidates for orthopedic applications. Here, we investigated the effect of Mg ions on the cellular properties of osteoblasts. Cytotoxicity tests on osteoblasts confirmed that no cytotoxic effects were found up to a supplementing Mg ion concentration of 10 mM. Mg ions at a concentration of 5 mM increased the migration and invasiveness of osteoblasts. To investigate the stimulatory effect of Mg ions on cell motility in scaffolds, we fabricated 10 wt% Mg ion-containing polycaprolactone (PCL) scaffolds, using the wire-network molding (WNM) technique. Mg ion-containing scaffolds persistently released Mg ions at a concentration of 5 mM in the media after pre-incubation. Furthermore, increased cell motility was confirmed in Mg ion-containing scaffolds by quantification of genomic DNA and protein content. Our results provide an important basis for the function of Mg ions and their effect on cell motility, and propose a novel role for Mg ions in scaffold applications.

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Acknowledgements

This work was supported by Chungnam National University and the National Research Foundation of Korea within the Basic Science Research Program through the Ministry of Education under Grant NRF-2014R1A1A2009489.

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Authors and Affiliations

  1. Department of Mechatronics Engineering, College of Engineering, Chungnam National University, Daejeon, 34134, Republic of Korea

    Ki-Jung Kim & Seok-Jo Yang

  2. Department of Biochemistry, College of Natural Sciences, Chungnam National University, Daejeon, 34134, Republic of Korea

    Sunkyung Choi & Kee K. Kim

  3. Division of Mechanical and Automotive Engineering, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk, 54538, Republic of Korea

    Yong Sang Cho & Young-Sam Cho

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  1. Ki-Jung Kim
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  2. Sunkyung Choi
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Correspondence to Young-Sam Cho or Kee K. Kim.

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Kim, KJ., Choi, S., Sang Cho, Y. et al. Magnesium ions enhance infiltration of osteoblasts in scaffolds via increasing cell motility. J Mater Sci: Mater Med 28, 96 (2017). https://doi.org/10.1007/s10856-017-5908-5

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  • DOI: https://doi.org/10.1007/s10856-017-5908-5

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