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Characterization of fibril dynamics on three timescales by solid-state NMR

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A Correction to this article was published on 08 March 2018

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Abstract

A multi-timescale analysis of the backbone dynamics of HET-s (218–289) fibrils is described based on multiple site-specific R 1 and R 1ρ data sets and S 2 measurements via REDOR for most backbone 15N and 13Cα nuclei. 15N and 13Cα data are fitted with motions at three timescales. Slow motion is found, indicating a global fibril motion. We further investigate the effect of 13C–13C transfer in measurement of 13Cα R 1. Finally, we show that it is necessary to go beyond the Redfield approximation for slow motions in order to obtain accurate numerical values for R 1ρ.

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Change history

  • 08 March 2018

    In our recent publication (Smith et al., J Biomol NMR 65:171–191, 2016) on the dynamics of HET-s(218–289), we reported on page 176, that calculation of solid-state NMR R1ρ rate constants using analytical equations based on Redfield theory (Kurbanov et al., J Chem Phys 135:184104:184101–184109, 2011) failed when the correlation time of motion becomes too long.

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Acknowledgments

This work has been supported by the Swiss National Science Foundation (Grants 200020_146757 and 200020_159797). We would also like to acknowledge thorough and carefully considered comments from a reviewer.

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  1. Physical Chemistry, ETH Zürich, Vladimir-Prelog-Weg 2, 8093, Zurich, Switzerland

    Albert A. Smith, Emilie Testori, Riccardo Cadalbert, Beat H. Meier & Matthias Ernst

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  1. Albert A. Smith
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  2. Emilie Testori
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Correspondence to Beat H. Meier or Matthias Ernst.

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A correction to this article is available online at https://doi.org/10.1007/s10858-018-0170-9.

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Smith, A.A., Testori, E., Cadalbert, R. et al. Characterization of fibril dynamics on three timescales by solid-state NMR. J Biomol NMR 65, 171–191 (2016). https://doi.org/10.1007/s10858-016-0047-8

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