Abstract
The use of fluorescent nanomaterials with good photostability and biocompatibility in live imaging of cells has gained increased attention. Even though several imaging techniques have been reported for mammalian cells, very limited literatures are available for nanomaterial based live imaging in plant system. We studied the uptake ability of two different nanomaterials, the highly photostable CdSe quantum dots and highly biocompatible FITC-labeled silica nanoparticles by rice seedlings which could provide greater opportunities for developing novel in vivo imaging techniques in plants. The effects of these nanomaterials on rice seed germination have also been studied for analyzing their phytotoxic effects on plants. We observed good germination of seeds in the presence of FITC-labeled silica nanoparticles whereas germination was arrested with quantum dots. The uptake of both the nanomaterials has been observed with rice seedlings, which calls for more research for recommending their safe use as biolabels in plants.
Similar content being viewed by others
References
Lin Y-S, Tsai C-P, Huang H-Y, Kuo C-T, Hung Y, Huang D-M, Chen Y-C, Mou C-Y (2005) Well ordered mesoporous silica nanoparticles as cell markers. Chem Mater 17:4570–4573
Santra S, Yang H, Dutta D, Stanley JT, Holloway PH, Tan W, Moudgil BM, Mericle RA (2004) TAT conjugated, FITC doped silica nanoparticles for bioimaging applications. Chem Commun 24:2810–2811
Hoecke KV, De Schamphelaere KAC, der Meeren PV, Lucas S, Janssen CR (2008) Ecotoxicity of silica nanoparticles to the green algae Pesudokirchneriella subcapitata: Importance of surface area. Environ Toxicol Chem 27:1948–1957
Wei C, Zhang Y, Guo J, Han B, Yang X, Yuan J (2010) Effects of silica nanoparticles on growth and photosynthetic pigment contents of Scenedesmus obliquus. J Environ Sci 22:155–160
Lin B-S, Diao S-Q, Li C-H, Fang L-J, Qiao S-C, Min Y (2004) Effect of TMS (nanostructured silicon dioxide) on growth of Changbai larch seedlings. J For Res 15:138–140
Nair R, Varghese SH, Nair BG, Maekawa T, Yoshida Y, Sakthi Kumar D (2010) Nanoparticulate material delivery to plants. Plant Sci 179:154–163
Torney F, Trewyn BG, Lin VS-Y, Wang K (2007) Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nat Nanotech 2:295–300
Hischemoller A, Nordmann J, Ptacek P, Mummenhoff K, Haase M (2009) In-vivo imaging of the uptake of upconversion nanoparticles by plant roots. J Biomed Nanotech 5:278–284
Bailey RE, Smith AM, Shuming N (2004) Quantum dots in biology and medicine. Phys E 25:1–12
Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sndaresan G, Wu AM, Gambhir SS, Weiss S (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307:538–544
Lidke DS, Nagy P, Heintzmann R, Arndt-Jovin DJ, Post JN, Grecco HE, Jares-Erijman EA, Jovin TM (2004) Quantum dot ligands provide new insights into erb/HER receptor-mediated signal transduction. Nat Biotechnol 22:198–203
Guo G, Liu W, Liang J, He Z, Xu H, Yang X (2007) Proing the cytotoxicity of CdSe quantum dots with surface modification. Mater Lett 61:1641–1644
Gagne F, Auclair J, Turcotte P, Fournier M, Gagnon C, Sauve S, Blaise C (2008) Ecotoxicity of CdTe quantum dots to freshwater mussels: impacts on immune system, oxidative stress and genotoxicity. Aquat Toxicol 86:333–340
Lin S, Bhattacharya P, Rajapakse NC, Brune DE, Ke PC (2009) Efffects of quantum dots adsorption on algal photosynthesis. J Phys Chem C 113:10962–10966
Etxeberria E, Gonzalez P, Baroja-Fernandez E, Romero JP (2006) Fluid phase endocytic uptake of artificial nano-spheres and fluorescent quantum dots by sycamore cultured cells. Plant Signaling Behav 1:196–200
Ravindran S, Kim S, Martin R, Lord EM, Ozkan CS (2005) Quantum dots as biolabels for the localization of a small plant adhesion protein. Nanotechnology 16:1–4
Muller F, Houben A, Barker PE, Xiao Y, Kas JA, Melzer M (2006) Quantum dots-a versatile tool in plant science. J Nanobiotechnol 4:5–10
Eggenberger K, Frey N, Zienicke B, Siebenbrock J, Schunck T, Fischer R, Brase S, Birtalan E, Nann T, Nick P (2010) Use of nanoparticles to study and manipulate plant cells. Adv Eng Mater 12:B406–B412
Hu Y, Li J, Ma L, Peng Q, Feng W, Zhang L, He S, Yang F, Huang J, Li L (2010) High efficiency transport of quantum dots into plant roots with the aid of silwet L-77. Plant Physiol Biochem 48:703–709
Boatman EM, Lisensky GC (2005) A safer, easier, faster synthesis for CdSe quantum dot nanocrystals. J Chem Educ 11:1697–1699
Xie R, Kolb U, Li J, Basche T, Mews A (2005) Synthesis and characterization of highly luminescent CdSe-Core CdS/Zn0.5Cd0.5S/ZnS multishell nanocrystals. J Am Chem Soc 127:7480–7488
Chan WCW, Nie SM (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016–2018
Han Y, Jiang J, Lee SS, Ying JY (2008) Reverse microemulsion mediated synthesis of silica coated gold and silver nanoparticles. Langmuir 24:5842–5848
Zhao L, Zhao Y, Han Y (2010) Pore fabrication in various silica-based nanoparticles by controlled etching. Langmuir 26:11784–11789
Liu Z, Jiao Y, Wang Y, Zhou C, Zhang Z (2008) Polysaccharides-based nanoparticles as drug delivery systems. Adv Drug Deliv Rev 60:1650–1662
Acknowledgement
Remya Nair and Aby C. Poulose thank the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan for the financial support given as Monbukagakusho fellowship
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nair, R., Poulose, A.C., Nagaoka, Y. et al. Uptake of FITC Labeled Silica Nanoparticles and Quantum Dots by Rice Seedlings: Effects on Seed Germination and Their Potential as Biolabels for Plants. J Fluoresc 21, 2057–2068 (2011). https://doi.org/10.1007/s10895-011-0904-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10895-011-0904-5