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Thioglycolic Acid-Capped CdS Quantum Dots Conjugated to α-Amylase as a Fluorescence Probe for Determination of Starch at Low Concentration

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Abstract

In the present research, water soluble thioglycolic acid-capped CdS quantum dots (QDs) were synthesized by chemical precipitation method. The characteristics of prepared quantum dots were determined using X-Ray Diffraction (XRD) and Transmission Electron Microscopy (TEM). The obtained results revealed that CdS QDs have 5.60 nm crystallite size, hexagonal wurtzite structure and spherical morphology with less than 10 nm diameter. The photoluminescence (PL) spectroscopy was performed in order to study the effect of the presence of starch solutions. Blue emission peaks were positioned at 488 nm and its intensity quenched by increasing the concentration of starch solutions. The result of PL quenches in range of studied concentrations (0–100 ppm) was best described by Michaelis-Menten model. The amount of Michaelis constant (Km) for immobilized α-amylase in this system was about 68.08 ppm which showed a great tendency of enzyme to hydrolyze the starch as substrate. Finally, the limit of detection (LOD) was found to be about 2.24 ppm.

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Acknowledgments

The authors hereby gratefully acknowledge Professor Farzaneh Vahabzadeh; all the laboratory facilities were provided under the aegis of her.

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Authors and Affiliations

  1. Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran

    Mahnoush Tayebi, Mohammad Tavakkoli Yaraki, Azadeh Mogharei & Mahnaz Ahmadieh

  2. Marquette University School of Dentistry, Milwaukee, WI, 53233, USA

    Mohammadreza Tahriri & Lobat Tayebi

  3. Dental Biomaterials Department, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

    Mohammadreza Tahriri

  4. Biomaterials Group, Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran

    Mohammadreza Tahriri

  5. Electrical and Computer Engineering Department, North Carolina State University, Raleigh, NC, 27606, USA

    Daryoosh Vashaee

  6. Department of Engineering Science, University of Oxford, Oxford, OX1 3PJ, UK

    Lobat Tayebi

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  1. Mahnoush Tayebi
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  2. Mohammad Tavakkoli Yaraki
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  3. Azadeh Mogharei
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  4. Mahnaz Ahmadieh
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  5. Mohammadreza Tahriri
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  6. Daryoosh Vashaee
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  7. Lobat Tayebi
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Corresponding authors

Correspondence to Mohammad Tavakkoli Yaraki or Mohammadreza Tahriri.

Additional information

M. Tayebi and M. Tavakkoli Yaraki contributed equally to this work.

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Tayebi, M., Tavakkoli Yaraki, M., Mogharei, A. et al. Thioglycolic Acid-Capped CdS Quantum Dots Conjugated to α-Amylase as a Fluorescence Probe for Determination of Starch at Low Concentration. J Fluoresc 26, 1787–1794 (2016). https://doi.org/10.1007/s10895-016-1870-8

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  • DOI: https://doi.org/10.1007/s10895-016-1870-8

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