Abstract
This study is the first report on the separation and reusability of ApoUnaG protein, indicating excellent fluorescence response with high affinity and specificity toward unconjugated bilirubin (UC-BR) molecules, from the UnaG-UC-BR complex structure. The fluorescence properties of the UnaG-UC-BR complex (holo-UnaG) are studied by addition of different metal ions to perform possible interactions with holo-UnaG through absorbance and emission spectra. After addition of metal ions, some changes with respect to the type of metal ions are observed in fluorescence intensity of the holo-UnaG. When compared to metal ions, an excellent quenching response is sighted in the presence of Cu2+ ions by binding with UC-BR in the UnaG-UC-BR complex structure. Obtained non-fluorescence holo-UnaG-Cu2+ complex mixture is passed through Ni–NTA agarose to remove the ingredients such as Cu2+, UC-BR and Cu2+-UC-BR coordination complex from holo-UnaG. From the obtained experiments, it is concluded that Cu2+ ion can be used as an agent for the recovery of ApoUnaG protein via binding with UC-BR molecules.
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Eczacioglu, N., Yilmaz, B., Ulusu, Y. et al. Recovery and Reusability of ApoUnaG Fluorescence Protein from the Unconjugated Bilirubin Complex Structure. J Fluoresc 30, 497–503 (2020). https://doi.org/10.1007/s10895-020-02519-w
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DOI: https://doi.org/10.1007/s10895-020-02519-w