Abstract
Electrospinning is one of the promising techniques to fabricate the nanofiber based scaffold for bone regeneration applications. In this study, firstly sol–gel method was employed to synthesize 5 % of Ag doped hydroxyapatite and also 10 wt% of polyvinyl alcohol solution was prepared for the electrospun process. For the first time, we have successfully fabricated the composite nanofibers in the combination of various concentration of Ag doped hydroxyapatite such as 1, 2, 3, and 5 wt% with polyvinyl alcohol solution. The developed Ag doped hydroxyapatite-polyvinyl alcohol composites were further characterized by Fourier transform infrared spectroscopy and powder-X-ray diffraction analysis to examine the characteristic functional groups and phase composition of Ag doped hydroxyapatite embedded into polyvinyl alcohol matrix. The uniform distribution of Ag doped hydroxyapatite in polyvinyl alcohol polymer with nanofiber diameter of 188–242 nm range was confirmed by high resolution transmission electron microscope and dynamic light scattering analysis, also the chemical/elemental composition was observed by scanning electron microscopy-energy dispersive spectroscopy analysis. The antibacterial activity was evaluated for the fabricated Ag doped hydroxyapatite polyvinyl alcohol composite nanofibers by using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) pathogens and the results demonstrated that E. coli exhibits excellent zone of inhibition than S. aureus due to its lesser cell wall thickness. The hemocompatibility study proves that the developed composite nanofibers are blood compatible and showed the hemolytic ratio of less than 5 %. In addition to this, in vitro bioactivity assessment was carried out for 7 days by immersing in simulated body fluid solution to generate the dense apatite layer on their surfaces which was further examined by X-ray diffraction and scanning electron microscopy-energy dispersive spectroscopy analysis. Hence, these electrospun fabricated Ag doped hydroxyapatite-polyvinyl alcohol composite nanofibers will acts as a potential scaffold material for tissue engineering applications.
Graphical Abstract
Graphical abstract of electrospun fabrication of Ag@HAP-PVA composite nanofibers and its in vitro biological evaluations.
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References
Song JH, Kim HE, Kim HW (2008) Electrospun fibrous web of collagen–apatite precipitated nanocomposite for bone regeneration. J Mater Sci Mater Med 19:2925–2932
Rho JY, Spearing LK, Zioupos P (1998) Mechanical properties and the hierarchical structure of bone. Med Eng Phys 20:92–102
Goldberg VM, Stevenson S (1987) Natural history of autografts and allografts. Clin Orthop Relat Res 225:7–16
Kaeding CC, Aros B, Pedroza A, Pifel E, Amendola A, Andrish JT, Wright RW (2011) Allograft versus autograft anterior cruciate ligament reconstruction. Sports Health Multidiscip Approach 3:73–81
Pascu EI, Stokes J, McGuinness GB (2013) Electrospun composites of PHBV, silk fibroin and nano-hydroxyapatite for bone tissue engineering. Mater Sci Eng C 33:4905–4916
Murugan R, Ramakrishna S (2005) Development of nanocomposites for bone grafting. Compos Sci Technol 65:2385–2406
Leung V, Ko F (2011) Biomedical applications of nanofibers. Polym Adv Technol 22:350–365
Kanani AG, Bahrami SH (2010) Review on electrospun nanofibers scaffold and biomedical applications. Trends Biomater Artif Organs 24:93–115
Khang GS, Lee J, Kim MS, Lee HB (2006) In: Webster S (ed), Biomaterials: tissue engineering and scaffold. Wiley Press, New York
Chaignaud BE, Langer R, Vacanti JP (1997) In: Atala A and Mooney DJ (ed), The history of tissue engineering using synthetic biodegradable polymer scaffolds and cells. Birkhauser, Boston
Hong KH, Park JL, Sul IH, Youk JH, Kang TJ (2006) Preparation of antimicrobial poly(vinyl alcohol) nanofibers containing silver nanoparticles. J Polym Sci B Polym Phys 44:2468–2474
Hong KH, Kang TJ (2006) Hydraulic permeabilities of PET and nylon 6 electrospun fiber webs. J Appl Polym Sci 100:167–177
Anjaneyulu U, Pattanayak DK, Vijayalakshmi U (2016) Snail shell derived natural hydroxyapatite: Effects on Nih-3t3 cells for orthopedic applications. Mater Manuf Process 31:206–216
Anjaneyulu U, Pattanayak DK, Vijayalakshmi U (2015) The facile and phase pure evaluations of nano hydroxyaptite powder by sol–gel method. Int J Chem Tech Res 7:1516–1520
Iqbal N, Kadir MRA, Malek NANN, Mahmood NH, Murali MR, Kamarul T (2012) Rapid microwave assisted synthesis and characterization of nanosized silver-doped hydroxyapatite with antibacterial properties. Mater Lett 89:118–122
Archana R, Rakesh CB, Duraipandy N, Kiran MS, Deepak KP (2014) Synthesis, phase stability of hydroxyapatite–silver composite with antimicrobial activity and cytocompatability. Ceram Int 40:10831–10838
Liao H, Qi R, Shen M, Cao X, Guo R, Zhang Y, Shi X (2011) Improved cellular response on multiwalled carbon nanotube-incorporated electrospun polyvinyl alcohol/chitosan nanofibrous scaffolds. Colloids Surf B Biointerfaces 84:528–535
Karim MR (2013) Fabrication of electrospun aligned nanofibers from conducting polyaniline copolymer/polyvinyl alcohol/chitosan oligossacaride in aqueous solutions. Synth Met 178:34–37
Sheikh FA, Barakat NA, Kanjwal MA, Park SJ, Park DK, Kim HY (2010) Synthesis of poly(vinyl alcohol) (PVA) nanofibers incorporating hydroxyapatite nanoparticles as future implant materials. Macromol Res 18:59–66
Kim GM, Asran AS, Michler GH, Simon P, Kim JS (2008) Electrospun PVA/HAp nanocomposite nanofibers: biomimetics of mineralized hard tissues at a lower level of complexity. Bioinsp Biomim 3:046003
Celebia H, Gurbuz M, Koparal S, Dogan A (2013) Development of antibacterial electrospun chitosan/poly(vinyl alcohol) nanofibers containing silver ion-incorporated HAP nanoparticles. Compos Interface 20:799–812
Suslu A, Albayrak AZ, Bayir E, Urkmez AS, Cocen U (2015) In vitro biocompatibility and antibacterial activity of electrospun Ag doped HAp-PHBV composite nanofibers. Int J Polym Mater 64:465–473
Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T (1990) Solutions able to reproduce in vivo surface‐structure changes in bioactive glass‐ceramic A‐W3. J Biomed Mater Res 24:721–734
Bahrami SH, Nouri M (2009) Chitosan-poly (vinyl alcohol) blend nanofibers: morphology, biological and antimicrobial properties. E-Polymers 9:1580–1591
Anjaneyulu U, Swaroop VK, Vijayalakshmi U (2016) Preparation and characterization of novel Ag doped hydroxyapatite–Fe3O4–chitosan hybrid composites and in vitro biological evaluations for orthopaedic applications. RSC Adv 6:10997–11007
Asran AS, Henning S, Michler GH (2010) Polyvinyl alcohol-collagen-hydroxyapatite biocomposite nanofibrous scaffold: mimicking the key features of natural bone at the nanoscale level. Polymer 51:868–876
Tan G, Saglam S, Emul E, Erdonmez D, Saglam N (2016) Synthesis and characterization of silver nanoparticles integrated in polyvinyl alcohol nanofibers for bionanotechnological applications. Turk J Biol 40:643–651
Calamak S, Aksoy EA, Ertas N, Erdogdu C, Sagıroglu M, Ulubayram K (2015) Ag/silk fibroin nanofibers: Effect of fibroin morphology on Ag+ release and antibacterial activity. Eur Polym J 67:99–112
Almajhdi FN, Fouad H, Khalil KA, Awad HM, Mohamed SHS, Elsarnagawy T, Albarrag AM, Jassir FFA, Abdo HS (2014) In-vitro anticancer and antimicrobial activities of PLGA/silver nanofiber composites prepared by electrospinning. J Mater Sci Mater Med 25:1045–1053
Tank K (2014) Nano hydroxyapatite and silver doped nano hydroxyapatite. Scholar’s Press, Mannheim, Germany
Tank KP, Chudasama KS, Thaker VS, Joshi MJ (2014) Pure and zinc doped nano-hydroxyapatite: Synthesis, characterization, antimicrobial and hemolytic studies. J Cryst Growth 401:474–479
Prodana M, Duta M, Ionita D, Bojin D, Stan MS, Dinischiotu A, Demetrescu I (2015) A new complex ceramic coating with carbon nanotubes, hydroxyapatite and TiO2 nanotubes on Ti surface for biomedical applications. Ceram Int 4:16318–16325
Yusuf MK, Emily KC, John KK, Cato TL (2007) In situ synthesized ceramic-polymer composites for bone tissue engineering: bioactivity and degradation studies. J Mater Sci 42:4183–4190
Mabrouk M, Mostafa AA, Oudadesse H, Mahmoud AA, El Gohary MI (2014) Effect of ciprofloxacin incorporation in PVA and PVA bioactive glass composite scaffolds. Ceram Int 40:4833–4845
Lijun K, Yuan G, Guangyuan L, Yandao G, Nanming Z, Xiufang Z (2006) A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering. Eur Polym J 42:3171–3179
Cziko M, Bogya ES, Barabas R, Bizo L, Stefan R (2013) In vitro biological activity comparison of some hydroxyapatite-based composite materials using simulated body fluid. Cent Eur J Chem 11:1583–1598
Kokubo T, Kim HM, Kawashita M (2003) Novel bioactive materials with different mechanical properties. Biomaterials 24:2161–2175
Gu YW, Khor KA, Cheang P (2004) Bone-like apatite layer formation on hydroxyapatite prepared by spark plasma sintering (SPS). Biomaterials 25:4127–4134
Acknowledgments
One of the author U. Vijayalakshmi acknowledges the DST, New Delhi, India (SB/FT/CS-091/2012) for granting financial support. Also the authors express their gratitude to VIT University for providing all the research facilities.
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Anjaneyulu, U., Priyadarshini, B., Nirmala Grace, A. et al. Fabrication and characterization of Ag doped hydroxyapatite-polyvinyl alcohol composite nanofibers and its in vitro biological evaluations for bone tissue engineering applications. J Sol-Gel Sci Technol 81, 750–761 (2017). https://doi.org/10.1007/s10971-016-4243-5
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DOI: https://doi.org/10.1007/s10971-016-4243-5