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In Vitro and In Silico Analysis of Novel ACE-Inhibitory Bioactive Peptides Derived from Fermented Goat Milk

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Abstract

In the study, two Lactobacillus cultures i.e. L. casei (NK9) and L. fermentum (LF) were studied for their proteolytic activity, di and tripeptidase activity, ACE-inhibitory activity and peptides production under optimized growth condition from fermented goat milk (Capra aegagrus hircus). NK9 and LF were found to be a strong proteolytic culture with 2.0% rate of inoculation after 48 h. LF (10 kDa retentate) produced maximum peptides among all the retentates of the fermented goat milk. Goat milk fermented with NK9 (10 kDa permeates) exhibited peptide sequence i.e. AFPEHK which had ACE inhibitory activity, matched with goat milk protein databases of AHTPDB. However, L. casei (NK9) and L. fermentum (LF) could be explored for the production of ACE inhibitory peptides from fermented goat milk.

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Authors and Affiliations

  1. Dairy Microbiology Department, Anand Agricultural University, Anand, Gujarat, 388110, India

    Heena Parmar & Subrota Hati

  2. Plant Biotechnology Department, Anand Agricultural University, Anand, Gujarat, 388110, India

    Amar Sakure

Authors
  1. Heena Parmar
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  2. Subrota Hati
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  3. Amar Sakure
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Correspondence to Subrota Hati.

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This study does not have any conflict of interest with co-authors i.e. Ms. Heena Parmar, Dr. Subrota Hati and Dr. Amar Sakure.

Research Involving Human and Animal Participants

This article does not contain any studies with human participants performed by any of the authors. We did not involve any animals as well human for this study.

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Parmar, H., Hati, S. & Sakure, A. In Vitro and In Silico Analysis of Novel ACE-Inhibitory Bioactive Peptides Derived from Fermented Goat Milk. Int J Pept Res Ther 24, 441–453 (2018). https://doi.org/10.1007/s10989-017-9630-4

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  • DOI: https://doi.org/10.1007/s10989-017-9630-4

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