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Improvement of the Antioxidant Activity of Fenugreek Protein Isolates by Lactococcus lactis Fermentation

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Abstract

This study investigated the antioxidant capacity of fenugreek protein isolate and its improvement by Lc. lactis fermentation through bioactive peptides production and the effect of molecular weight variation on fenugreek fractions antioxidant activity. Fenugreek protein isolate showed a significant increase of antioxidant and radical scavenging activity after 24 h of fermentation, by about 23.7, 42.9 and 40% for respectively antioxidant activity coefficient AAC, DPPH and ABTS radical scavenging activity. FI fermentation led to a hydrolysis of peptide bands with MW > 35 kDa and a generation of new bands with a MW < 25 kDa. A significant reduction in molecular-mass distribution of hydrolysates and a great increase of total free amino acids content, especially an increase on isoleucine, leucine, glutamic acid, serine, histidine, glutamine and lysine was noted. The infrared results showed that different reactions may take place after fermentation, and showed an increase of proteins, amides and aromatic compounds. However, fenugreek fraction (F2) with MW 15–50 kDa presented the highest activity instead of fraction (F1) with lower MW. Lc. lactis had the ability to degrade and convert fenugreek proteins into bioactive peptides that contribute positively in the improvement of antioxidant activity of FI and fractions. FI presents a significant antioxidant activity and thus, can be considered as a potential source of high added value natural antioxidants and may be employed as a functional food ingredient with good potential applications in food products.

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Authors and Affiliations

  1. Laboratory of Microbial Ecology and Technology (LETMI), National Institute of Applied Sciences and Technology (INSAT), BP: 676, 1080, Tunis, Tunisia

    Khaoula Setti, Faten Kachouri & Moktar Hamdi

  2. Superior School of Food Industry at Tunis (ESIAT), 58 Street Alain Savary, 1003, Tunis, Tunisia

    Khaoula Setti, Faten Kachouri & Moktar Hamdi

  3. Carthage University, Avenue de la République, Amilcar, BP 77, 1054, Tunis, Tunisia

    Khaoula Setti, Faten Kachouri & Moktar Hamdi

Authors
  1. Khaoula Setti
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  2. Faten Kachouri
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  3. Moktar Hamdi
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Correspondence to Khaoula Setti.

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Setti, K., Kachouri, F. & Hamdi, M. Improvement of the Antioxidant Activity of Fenugreek Protein Isolates by Lactococcus lactis Fermentation. Int J Pept Res Ther 24, 499–509 (2018). https://doi.org/10.1007/s10989-017-9636-y

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  • DOI: https://doi.org/10.1007/s10989-017-9636-y

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