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Isolation and Identification of a Novel Antioxidant Peptide from Chickpea (Cicer arietinum L.) Sprout Protein Hydrolysates

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Abstract

Chickpea (Cicer arietinum L.) is the second most widely cultivated leguminous plant in the world. In this study, the chickpea sprout protein (CSP) was hydrolyzed by various enzymes (trypsin, neutrase, alcalase and papain). The neutrase fraction (NF) which exhibits the best antioxidant activity with a high degree of hydrolysis (DH) was purified by ion-exchange chromatography (IEC), gel filtration chromatography (GFC), and reverse phase high-performance liquid chromatography (RP-HPLC). A novel antioxidant peptide (NF2-4-1) was identified to be Leu-Thr-Glu-IIe-IIe-Pro with 685.41 Da molecular weight utilized matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The IC50 values of NF2-4-1 was 0.24 ± 0.06 mg/mL on 1, 1-diphenyl-2-picrylhydrazyl (DPPH˙) and 0.57 ± 0.04 mg/mL on hydroxyl radicals (·OH) scavenging activity, respectively. Therefore, the results of this study demonstrate that chickpea sprouts can be used as a source of natural antioxidant peptides for food and nutraceutical applications.

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Acknowledgements

This research was funded by the Program for National Science and Technology Major Project of China, grant number 2017ZX09301045 and “The light of the western” talent training plan: Standardized Planting Techniques of Repellent Vernonia and Its Collection, Preservation and Popularization (Y92S201401), Planting of Hotan Rose and Its Polyphenols Application (Y92S211401); It is also supported by“Tian Shan Excellence Project” in 2017, and “western young scholars program provided by CAS (No. CAS-LWC-2017-1)”.

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Authors and Affiliations

  1. Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing South Road 40-1, Ürümqi, 830011, Xinjiang, China

    Ahmidin Wali, Yasen Mijiti, Gao Yanhua, Abulimiti Yili, Haji Akber Aisa & Adilijiang Kawuli

  2. University of Chinese Academy of Science, Beijing, 100039, China

    Ahmidin Wali & Yasen Mijiti

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  1. Ahmidin Wali
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  2. Yasen Mijiti
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  3. Gao Yanhua
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  4. Abulimiti Yili
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Correspondence to Abulimiti Yili.

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Wali, A., Mijiti, Y., Yanhua, G. et al. Isolation and Identification of a Novel Antioxidant Peptide from Chickpea (Cicer arietinum L.) Sprout Protein Hydrolysates. Int J Pept Res Ther 27, 219–227 (2021). https://doi.org/10.1007/s10989-020-10070-2

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  • DOI: https://doi.org/10.1007/s10989-020-10070-2

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