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Enhanced expression of adipocyte-type fatty acid binding protein in murine lymphocytes in response to dexamethasone treatment

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Abstract

Fatty acids have a great influence on the process of lymphocyte apoptosis which is considered as a modulating factor of immune response in both humans and animals. However the mechanism underlying the function of fatty acids in the process of lymphocyte apoptosis is not fully understood. In this study we show that the appearance of adipocyte-type fatty acid binding protein (A-FABP) is induced upon administration of dexamethasone (DEX) in both in vivo and cultured lymphocytes, and its distinct nuclear localization occurs in close relation to the DEX-induced apoptosis process. In immuunohistochemistry of mouse spleen, A-FABP-immunoreactivity starts to occur 3 h after DEX stimulation, and it massively localizes in the nucleus 8 h after the treatment, while no A-FABP-immunoreactivity is discerned in the lymphocytes of normal as well as 24 h post-injection spleen. In the murine T-cell leukemia CTLL-2 cells, A-FABP-immunoreactivity is also induced in both of the cytoplasm and nucleus when the apoptosis is induced by IL-2 retrieval together with DEX treatment, while in the presence of IL-2 A-FABP-immunoreactivity is confined to the cytoplasm with DEX trreatment. On the other hand, A-FABP-immunoreactivity is not detected by IL-2 retrieval alone. The present findings altogether suggest that A-FABP and its ligands, fatty acids, play an important role in the process of apoptosis and the immune modulation induced by DEX.

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References

  1. Rudolph IL, Kelley DS, Klasing KC, Erickson KL: Regulation of cellular differentiation and apoptosis by fatty acids and their metabolites. Nutr Res 21: 381–393, 2001

    Article  PubMed  CAS  Google Scholar 

  2. Scorrano L, Penzo D, Petronilli V, Pagano F, Bernardi P: Arachidonic acid causes cell death through the mitochondrial permeability transition. Implications for tumor necrosis factor-alpha aopototic signaling. J Biol Chem 276: 12035–12040, 2001

    Article  PubMed  CAS  Google Scholar 

  3. Paumen MB, Ishida Y, Muramatsu M, Yamamoto M, Honjo T: Inhibition of carnitine palmitoyltransferase I augments sphingolipid synthesis and palmitate-induced apoptosis. J Biol Chem 272: 3324–3329, 1997

    Article  PubMed  CAS  Google Scholar 

  4. Finstad HS, Drevon CA, Kulseth MA, Synstad AV, Knudsen E, Kolset SO: Cell proliferation, apoptosis and accumulation of lipid droplets in U937-1 cells incubated with eicosapentaenoic acid. Biochem J 336(Pt 2): 451–459, 1998

    PubMed  CAS  Google Scholar 

  5. Yano M, Kishida E, Iwasaki M, Kojo S, Masuzawa Y: Docosahexaenoic acid and vitamin E can reduce human monocytic U937 cell apoptosis induced by tumor necrosis factor. J Nutr 130: 1095–1101, 2000

    PubMed  CAS  Google Scholar 

  6. Switzer KC, McMurray DN, Morris JS, Chapkin RS: (n−3) Polyunsaturated fatty acids promote activation-induced cell death in murine T lymphocytes. J Nutr 133: 496–503, 2003

    PubMed  CAS  Google Scholar 

  7. Kitanaka N, Owada Y, Abdelwahab SA, Iwasa H, Sakagami H, Watanabe M, Spener F, Kondo H: Specific localization of epidermal-type fatty acid binding protein in dendritic cells of splenic white pulp. Histochem Cell Biol 120: 465–473, 2003

    Article  PubMed  CAS  Google Scholar 

  8. Thome M, Tschopp J: Regulation of lymphocyte proliferation and death by FLIP. Nat Rev Immunol 1: 50–58, 2001

    Article  PubMed  CAS  Google Scholar 

  9. Abdelwahab SA, Owada Y, Kitanaka N, Iwasa H, Sakagami H, Kondo H: Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide. Histochem Cell Biol 119: 469–475, 2003

    Article  PubMed  CAS  Google Scholar 

  10. Owada Y, Yoshimoto T, Kondo H: Spatio-temporally differential expression of genes for three members of fatty acid binding proteins in developing and mature rat brains. J Chem Neuroanat 12: 113–122, 1996

    Article  PubMed  CAS  Google Scholar 

  11. Owada Y, Suzuki R, Iwasa H, Spener F, Kondo H: Localization of epidermal-type fatty acid binding protein in the thymic epithelial cells of mice. Histochem Cell Biol 117: 55–60, 2002

    Article  PubMed  CAS  Google Scholar 

  12. Watanabe M, Ono T, Kondo H: Immunohistochemical studies on the localisation and ontogeny of heart fatty acid binding protein in the rat. J Anat 174: 81–95, 1991

    PubMed  CAS  Google Scholar 

  13. Owada Y, Abdelwahab SA, Suzuki R, Iwasa H, Sakagami H, Spener F, Kondo H: Localization of epidermal-type fatty acid binding protein in alveolar macrophages and some alveolar type II epithelial cells in mouse lung. Histochem J 33: 453–457, 2001

    Article  PubMed  CAS  Google Scholar 

  14. Iwata M, Ohoka Y, Kuwata T, Asada A: Regulation of T cell apoptosis via T cell receptors and steroid receptors. Stem Cells 14: 632–641, 1996

    Article  PubMed  CAS  Google Scholar 

  15. Andreau K, Lemaire C, Souvannavong V, Adam A: Induction of apoptosis by dexamethasone in the B cell lineage. Immunopharmacology 40: 67–76, 1998

    Article  PubMed  CAS  Google Scholar 

  16. Xie H, Seward RJ, Huber BT: Cytokine rescue from glucocorticoid induced apoptosis in T cells is mediated through inhibition of IkappaBalpha. Mol Immunol 34: 987–994, 1997

    Article  PubMed  CAS  Google Scholar 

  17. Tan NS, Shaw NS, Vinckenbosch N, Liu P, Yasmin R, Desvergne B, Wahli W, Noy N: Selective cooperation between fatty acid binding proteins and peroxisome proliferator-activated receptors in regulating transcription. Mol Cell Biol 22: 5114–5127, 2002

    Article  PubMed  CAS  Google Scholar 

  18. Tautenhahn A, Brune B, von Knethen A: Activation-induced PPARgamma expression sensitizes primary human T cells toward apoptosis. J Leukoc Biol 73: 665–672, 2003

    Article  PubMed  CAS  Google Scholar 

  19. Padilla J, Leung E, Phipps RP: Human B lymphocytes and B lymphomas express PPAR-gamma and are killed by PPAR-gamma agonists. Clin Immunol 103: 22–33, 2002

    Article  PubMed  CAS  Google Scholar 

  20. Phillips M, Djian P, Green H: The nucleotide sequence of three genes participating in the adipose differentiation of 3T3 cells. J Biol Chem 261: 10821–10827, 1986

    PubMed  CAS  Google Scholar 

  21. Guthmann F, Hohoff C, Fechner H, Humbert P, Borchers T, Spener F, Rustow B: Expression of fatty-acid-binding proteins in cells involved in lung-specific lipid metabolism. Eur J Biochem 253: 430–436, 1998

    Article  PubMed  CAS  Google Scholar 

  22. Kovacs B, Gulya K: Differential expression of multiple calmodulin genes in cells of the white matter of the rat spinal cord. Brain Res Mol Brain Res 102: 28–34, 2002

    Article  PubMed  CAS  Google Scholar 

  23. Amri EZ, Ailhaud G, Grimaldi P: Regulation of adipose cell differentiation. II. Kinetics of induction of the aP2 gene by fatty acids and modulation by dexamethasone. J Lipid Res 32: 1457–1463, 1991

    PubMed  CAS  Google Scholar 

  24. Sun L, Nicholson AC, Hajjar DP, Gotto AM, Jr., Han J: Adipogenic differentiating agents regulate expression of fatty acid binding protein and CD36 in the J744 macrophage cell line. J Lipid Res 44: 1877–1886, 2003

    Article  PubMed  CAS  Google Scholar 

  25. Cook JS, Lucas JJ, Sibley E, Bolanowski MA, Christy RJ, Kelly TJ, Lane MD: Expression of the differentiation-induced gene for fatty acid-binding protein is activated by glucocorticoid and cAMP. Proc Natl Acad Sci USA 85: 2949–2953, 1988

    Article  PubMed  CAS  Google Scholar 

  26. Goleva E, Kisich KO, Leung DY: A role for STAT5 in the pathogenesis of IL-2-induced glucocorticoid resistance. J Immunol 169: 5934–5940, 2002

    PubMed  CAS  Google Scholar 

  27. Semenkovich CF: Fatty acid metabolism and vascular disease. Trends Cardiovasc Med 14: 72–76, 2004

    Article  PubMed  CAS  Google Scholar 

  28. Rossi A, Kapahi P, Natoli G, Takahashi T, Chen Y, Karin M, Santoro MG: Anti-inflammatory cyclopentenone prostaglandins are direct inhibitors of IkappaB kinase. Nature 403: 103–108, 2000

    Article  PubMed  CAS  Google Scholar 

  29. Harris SG, Phipps RP: Peroxisome proliferator-activated receptor gamma (PPAR-gamma) activation in naive mouse T cells induces cell death. Ann N Y Acad Sci 905: 297–300, 2000

    Article  PubMed  CAS  Google Scholar 

  30. De Santis ML, Hammamieh R, Das R, Jett M: Adipocyte-fatty acid binding protein induces apoptosis in DU145 prostate cancer cells. J Exp Ther Oncol 4: 91–100, 2004

    PubMed  CAS  Google Scholar 

  31. Zingarelli B, Sheehan M, Hake PW, O'Connor M, Denenberg A, Cook JA: Peroxisome proliferator activator receptor-gamma ligands, 15-deoxy-Delta(12,14)-prostaglandin J2 and ciglitazone, reduce systemic inflammation in polymicrobial sepsis by modulation of signal transduction pathways. J Immunol 171: 6827–6837, 2003

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Division of Histology, Department of Cell Biology, Graduate School of Medical Science, Tohoku University, Tohoku, Sendai, 980-8575, Japan

    Soha Abdelkawi Abdelwahab, Yuji Owada, Noriko Kitanaka, Hiroyuki Sakagami & Hisatake Kondo

  2. Department of Pathology, Graduate School of Medical Science, Tohoku University, Tohoku, Japan

    Masao Ono

  3. Department of Aging and Geriatric Dentistry, Graduate School of Dentistry, Tohoku University, Tohoku, Sendai, 980-8575, Japan

    Noriko Kitanaka & Makoto Watanabe

  4. Department of Biochemistry, University of Muenster, Muenster, 48149, Germany

    Anne Adida & Friedrich Spener

  5. Division of Histology, Department of Cell Biology, Graduate School of Medical Science, Tohoku Univeristy, 2-1 Seiryo-cho, Aoba-ku, Tohoku, Sendai, 980-8575, Japan

    Yuji Owada

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  1. Soha Abdelkawi Abdelwahab
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  2. Yuji Owada
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  3. Noriko Kitanaka
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  4. Anne Adida
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  6. Masao Ono
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  7. Makoto Watanabe
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  8. Friedrich Spener
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  9. Hisatake Kondo
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Correspondence to Yuji Owada.

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Abdelwahab, S.A., Owada, Y., Kitanaka, N. et al. Enhanced expression of adipocyte-type fatty acid binding protein in murine lymphocytes in response to dexamethasone treatment. Mol Cell Biochem 299, 99–107 (2007). https://doi.org/10.1007/s11010-005-9050-1

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  • DOI: https://doi.org/10.1007/s11010-005-9050-1

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