Abstract
The role of lipids and carbohydrates in the adaptation of Flammulina velutipes to hypothermia (5 to −5°C) in natural environments has been studied for the first time. The main changes are were found to occur in membrane lipids: the levels of sterols and glycolipids decreased, and the proportion of phospholipids with a high degree of nonsaturation (2.2) increased, which was due to predominance of two fatty acids, linoleic (35% of the total) and linolenic (50%). Phosphatidylcholine became the major phospholipid. Under hypothermic conditions, glycerol, known to have antifreeze properties, accumulated in the cell cytosol along with arabitol and trehalose. The significance of this biochemical strategy in the resistance of the fungus to temperatures below zero (i.e., to cryobiotic conditions) is discussed.
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REFERENCES
Wasser, S.P. and Weis, A.L., Medicinal Properties of Substances Occurring in Higher Basidiomycetes Mushrooms: Current Perspectives, Int. J. Med. Mushrooms, 1999, vol. 1, pp. 31–62.
Sakamoto, Y., Ando, A., Tamai, Y., Miura, K., and Yajima, T., Protein Expression during Fruit Body Induction of Flammulina velutipes under Reduced Temperature, Mycol. Res., 2002, vol. 106, no.2, pp. 222–227.
Ingold, C.T., Mycelium, Oidia and Sporophore Initials in Flammulina velutipes, Trans. Brit. Mycol. Soc., 1980, vol. 75, no.1, pp. 107–116.
Feofilova, E.P., Tereshina, V.M., Khokhlova, N.S., and Memorskaya, A.S., Different Mechanisms of the Biochemical Adaptation of Mycelial Fungi to Temperature Stress: Changes in the Cytosol Carbohydrate Composition, Mikrobiologiya, 2000, vol. 69, no.5, pp. 597–605.
Feofilova, E.P., Tereshina, V.M., Memorskaya, A.S., and Khokhlova, N.S., Different Mechanisms of the Biochemical Adaptation of Mycelial Fungi to Temperature Stress: Changes in the Lipid Composition, Mikrobiologiya, 2000, vol. 69, no.5, pp. 606–612.
Song, C.H. and Cho, K.Y., Effect of Low Temperature Shock Treatment on Sporophore Initiation, Lipid Profile and Nutrient Transport in Lentinula edodes, Mycologia, 1991, vol. 83, no.1, pp. 24–29.
Weinstein, R.N., Montiel, P.O., and Johnstone, K., Influence of Growth Temperature on Lipid and Soluble Carbohydrate Synthesis by Fungi Isolated from Fellfield Soil in the Maritime Antarctic, Mycologia, 2000, vol. 92, no.2, pp. 222–229.
Folch, J., Lees, M., and Sloane-Stanley, G.H.S., A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem., 1957, vol. 226, no.1, pp. 497–529.
Kates, M., Techniques of Lipidology. Isolation, Analysis and Identification of Lipids, Amsterdam: Elsevier, 1972.
Somogyi, H., Determination of Blood Sugar, J. Biol. Chem., 1945, vol. 160, p. 69.
Brobst, K.M., Gas-Liquid Chromatography of Trimethylsilyl Derivatives of Sugars Metody issledovaniya uglevodov (Methods for Studies of Carbohydrates), Khorlin, F.Ya., Ed., Moscow: Mir, 1975.
Sinensky, M., Homeoviscous Adaptation—a Homeostatic Process That Regulates the Viscosity of Membrane Lipids in Escherichia coli, Proc. Natl. Acad. Sci. USA, 1974, vol. 71, no.2, pp. 522–525.
Hazel, J.R., Thermal Adaptation in Biological Membranes: Is Homeoviscous Adaptation the Explanation?, Annu. Rev. Physiol., 1995, vol. 57, pp. 19–42.
Kitamoto, Y. and Gruen, H.E., Distribution of Cellular Carbohydrates during Development of Mycelium and Fruit Bodies of Flammulina velutipes, Plant. Physiol., 1976, vol. 58, pp. 485–491.
Feofilova, E.P., Tereshina, V.M., Memorskaya, A.S., Zav’yalova, L.A., and Maryshova, N.S., Changes in the Cytosol Carbohydrate Composition during the Cytodifferentiation Process in the Fungi of the Order Agaricales, Mikrobiologiya, 1999, vol. 68, no.3, pp. 356–361.
Jennings, D.H., Polyol Metabolism in Fungi, Adv. Microb. Physiol., 1984, vol. 25, pp. 149–193.
Ushatinskaya, R.S., Skrytaya zhizn’ i anabioz (Cryptic Life and Anabiosis), Moscow: Nauka, 1990.
Dijkema, C., Kester, H.C.M., and Visser, J., 13C NMR Studies of Carbon Metabolism in the Hyphal Fungus Aspergillus nidulans, Proc. Natl. Acad. Sci. USA, 1985, vol. 82, pp. 14–18.
Yakovlev, A.Yu. and Borovskii, G.B., Dihydrin-like Polypeptides of the Mycelium an Fruit Bodies of Xylotrophic Basidiomycetes under Hypothermic Conditions, Sovremennaya mikologiya v Rossii. Tez. dokl. I S”ezda mikologov Rossii (Modern Mycology in Russia. Abstr. I Congr. of the Mycologists of Russia), Moscow, 2002, p. 159.
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Translated from Mikrobiologiya, Vol. 74, No. 3, 2005, pp. 329–334.
Original Russian Text Copyright © 2005 by Tereshina, Memorskaya.
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Tereshina, V.M., Memorskaya, A.S. Adaptation of Flammulina velutipes to Hypothermia in Natural Environments: The Role of Lipids and Carbohydrates. Microbiology 74, 279–283 (2005). https://doi.org/10.1007/s11021-005-0063-2
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DOI: https://doi.org/10.1007/s11021-005-0063-2