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Characterization of microsatellites from cacao–Moniliophthora perniciosa interaction expressed sequence tags

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Abstract

Theobroma cacao L.–Moniliophthora perniciosa expressed sequence tags (ESTs) were converted into useful satellite markers for population analysis and genetic mapping. Forty-nine flanking primer pairs from TSH1188 (a resistant genotype) and Catongo (a susceptible genotype) ESTs were designed and screened for polymorphism analysis. Eleven were polymorphic, with an average of 3.81 alleles per locus and a total of 42 alleles. The satellite markers were tested on 21 cacao accessions and two bulked DNAs generated from 6 resistant and 6 susceptible plants from a segregating F2 (SCA6 × ICS1) population for witches’ broom resistance. These results show that EST-derived microsatellites (short sequence repeats, SSRs) in Theobroma cacao have many potential applications in linkage mapping and the planning of crosses.

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Acknowledgments

The work of LSL was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). This research was supported by the Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), and Ministère des Affaires Etrangères Français (MAE).

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Authors and Affiliations

  1. Centro de Pesquisas do Cacau, Cx. Postal 07, 45600-970, Itabuna, BA, Brazil

    L. S. Lima, K. P. Gramacho, U. V. Lopes, H. A. Zaidan & J. L. Pires

  2. CEPLAC/CEPEC/SEFIT, Cx. Postal 07, 45600-970, Itabuna, BA, Brazil

    K. P. Gramacho

  3. Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, km16, 45662-000, Ilheus, BA, Brasil

    A. S. Gesteira, F. A. Gaiotto, J. C. M. Cascardo & F. Micheli

  4. CIRAD, UMR DAP, Avenue Agropolis, 34398, Montpellier, France

    F. Micheli

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  1. L. S. Lima
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  2. K. P. Gramacho
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  3. A. S. Gesteira
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  4. U. V. Lopes
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  5. F. A. Gaiotto
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  6. H. A. Zaidan
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  7. J. L. Pires
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  8. J. C. M. Cascardo
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  9. F. Micheli
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Corresponding author

Correspondence to K. P. Gramacho.

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Lima, L.S., Gramacho, K.P., Gesteira, A.S. et al. Characterization of microsatellites from cacao–Moniliophthora perniciosa interaction expressed sequence tags. Mol Breeding 22, 315–318 (2008). https://doi.org/10.1007/s11032-008-9174-6

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  • DOI: https://doi.org/10.1007/s11032-008-9174-6

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