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Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars

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Abstract

Sharka is a severe apricot viral disease caused by the plum pox virus (PPV) and is responsible for large crop losses in many countries. Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived from Lito, described in the literature as resistant, crossed to the susceptible selection BO81604311 (San Castrese × Reale di Imola) was used to study the genetic control of resistance to PPV. A population of 118 individuals was phenotyped by inoculating both PPV-D and PPV-M strains in replicated seedlings and scored for 3 years. An additional set of 231 seedlings from the same cross was also phenotyped for 2 years. SSR-based linkage maps were used for quantitative trait locus (QTL) analysis. A major QTL of resistance to both PPV-M and PPV-D strains was found in the top half of the Lito linkage group 1, where a QTL was previously described in Stark Earli-Orange, the donor of Lito resistance. The LOD score was considerably enhanced when the recovery of plants from infection was taken into account. The results obtained in Lito were compared with those observed in a second apricot cross progeny (Harcot × Reale di Imola) in which QTL of resistance to sharka were also mapped in the same linkage group 1 for both PPV strains. Several models of resistance to sharka disease are discussed considering the segregation frequencies, the QTL alignment in the two maps and the information gathered from the literature.

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Acknowledgements

This research was supported by the Ministry of Education, University, and Scientific Research (MIUR), National Project PRIN 2006 “Positional cloning of sharka resistance candidate genes in apricot”. We acknowledge Dr Ross Ferguson, Plant & Food Research, New Zealand, for the critical reading of the manuscript.

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Authors and Affiliations

  1. Dipartimento di Colture Arboree, Università degli Studi di Bologna, Via Fanin 46, 40127, Bologna, Italy

    Luca Dondini, Marco Adami, Michela Guidarelli, Federica Gaiotti, Stefano Tartarini & Paola Negri

  2. Dipartimento di Scienze Agrarie e Ambientali, Università di Udine, Udine, Italy

    Orietta Lain, Vera Vendramin & Raffaele Testolin

  3. Dipartimento di Produzione Vegetale, Università di Milano, Milan, Italy

    Marisa Rizzo, Davide Vivoli, Filippo Geuna & Daniele Bassi

  4. Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università di Bari, Bari, Italy

    Francesco Palmisano, Alessandra Bazzoni, Mariantonietta Castellano & Vito Savino

  5. CNR, Istituto di Virologia Vegetale, Bari, Italy

    Donato Boscia

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  1. Luca Dondini
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  2. Orietta Lain
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  3. Vera Vendramin
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Correspondence to Luca Dondini.

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Dondini, L., Lain, O., Vendramin, V. et al. Identification of QTL for resistance to plum pox virus strains M and D in Lito and Harcot apricot cultivars. Mol Breeding 27, 289–299 (2011). https://doi.org/10.1007/s11032-010-9431-3

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  • DOI: https://doi.org/10.1007/s11032-010-9431-3

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