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Identification and fine mapping of a resistance gene to Magnaporthe oryzae in a space-induced rice mutant

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Abstract

Finding novel sources of resistance (R) to rice blast disease should facilitate breeding for improved resistance. The objectives of the present study were to evaluate reactions to blast and identify in a space-induced mutant an R gene to a representative isolate of rice blast pathogen. The mutant H4, its parent and twelve monogenic lines were evaluated for their responses to 35 isolates collected from Guangdong Province, China. H4 was found to be resistant to more isolates than its parent and the twelve monogenic lines, suggesting newly acquired resistance may be a function of one or more R genes. A representative isolate GD0193 was used to identify and map the R gene from H4. Genetic analysis revealed that resistance to the isolate GD0193 was controlled by a single dominant gene, designated Pi46(t). Linkage analysis using susceptible F2 individuals showed that Pi46(t) was mapped between the markers RM224 and RM27360 within 1.04 and 1.2 cM on the long arm of chromosome 11. Subsequently, Pi46(t) was delimited to an interval of approximately 183.7 kb flanked by the markers K67 and T94. These results provide essential information for the cloning of the Pi46(t) gene and will facilitate marker-assisted selection in rice breeding.

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Acknowledgments

We thank the Rice Research Institute, Guangdong Academy of Agricultural Sciences, for providing the seeds of Zhonger Ruanzhan. We are grateful to the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, for providing the monogenic lines in this study. This research was supported by the National 863 Program of China (2007AA100101), the National Key Technology Research & Development Program of China (2008BAD97B02), the Natural Science Foundation of Guangdong Province, China (815106400100002), and the earmarked fund for Modern Agro-industry Technology Research System.

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Authors and Affiliations

  1. College of Agriculture, South China Agricultural University, Guangzhou, 510642, China

    Wuming Xiao, Hui Wang, Tao Guo, Yongzhu Liu & Zhiqiang Chen

  2. Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Qiyun Yang & Xiaoyuan Zhu

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  1. Wuming Xiao
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  2. Qiyun Yang
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  3. Hui Wang
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  4. Tao Guo
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Correspondence to Zhiqiang Chen.

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Wuming Xiao and Qiyun Yang contributed equally to this work.

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Xiao, W., Yang, Q., Wang, H. et al. Identification and fine mapping of a resistance gene to Magnaporthe oryzae in a space-induced rice mutant. Mol Breeding 28, 303–312 (2011). https://doi.org/10.1007/s11032-010-9481-6

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