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Intervention of Acidophilus-casei dahi and Wheat bran against molecular alteration in colon carcinogenesis

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Abstract

An in vivo trial was conducted on seventy five rats allocated to three groups, first group was DMH control group, second group was Wheat bran-DMH group (WB-DMH) in which wheat bran was given along with DMH (1,2-dimethylhydrazine) injection, third group was Wheat bran-DMH-Ac Dahi group in which both wheat bran and Acidophilus-casei dahi (a probiotic microorganisms fermented dairy product) was given along with DMH injections. Animals received subcutaneous injections of DMH at a dose rate of 20 mg/kg body weight, once weekly for 15 weeks. The rats were dissected at 40th week of experiment and comet assay was done in colonic cells to assess the DNA damage. The c-myc and cox-2 expression was studied in rat tumour. A significant reduction in DNA damage (48.2%) was observed in WB-DMH-Ac Dahi group as compared to DMH control group (87.8%). The c-myc and cox-2 mRNA level was found highest in DMH control group as compared to WB-DMH and WB-DMH-Ac Dahi group. The results of present study show the enhanced protective potential of Acidophilus-casei and wheat bran against DMH induced molecular alteration in colonic cells during carcinogenesis.

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Abbreviations

Ac Dahi:

Acidophilus-casei dahi

WB:

Wheat bran

DMH:

1,2-Dimethylhydrazine

References

  1. Parker SL, Tong T, Bolden S, Wong PA (1996) Cancer statistics. CA Cancer J Clin 46:5–27. doi:10.3322/canjclin.46.1.5

    Article  CAS  PubMed  Google Scholar 

  2. Ravnik-Glavac M, Cerar A, Glavac D (2000) Animal model in the study of colorectal carcinogenesis. Pflugers Arch 440(suppl. 5):R55–R57. doi:10.1007/s004240000005

    Article  CAS  PubMed  Google Scholar 

  3. Luceri C, De Filippo C, Caderni G, Gambacciani L, Salvadori M, Giannini A et al (2000) Detection of somatic DNA alterations in azoxymethane-induced F344 rat colon tumors by random amplified polymorphic DNA analysis. Carcinogenesis 21:1753–1756. doi:10.1093/carcin/21.9.1753

    Article  CAS  PubMed  Google Scholar 

  4. Kishimoto Y, Morisawa T, Hosoda A, Shiota G, Kawasaki H, Hasegawa J (2002) Molecular changes in the early stage of colon carcinogenesis in rats treated with azoxymethane. J Exp Clin Cancer Res 21:203–211

    CAS  PubMed  Google Scholar 

  5. Blum CA, Tanaka T, Zhong X, Li Q, Dashwood WM, Pereira C (2003) Mutational analysis of Ctnnb1 and Apc in tumors from rats given 1,2-dimethylhydrazine or 2-amino-3-methylimidazo[4, 5- f]quinoline: mutational hotspots and the relative expression of beta-catenin and c-jun. Mol Carcinog 36:195–203. doi:10.1002/mc.10112

    Article  CAS  PubMed  Google Scholar 

  6. Bird RP, Good CK (2000) The significance of aberrant crypt foci in understanding the pathogenesis of colon cancer. Toxicol Lett 112:395–402. doi:10.1016/S0378-4274(99)00261-1

    Article  PubMed  Google Scholar 

  7. Kountouras J, Boura P, Lygidakis NJ (2000) New concept of molecular biology for colon carcinogenesis. Hepatogastroenterology 47:1291–1297

    CAS  PubMed  Google Scholar 

  8. Gryfe R, Swallow C, Bapat B, Redston M, Gallinger S, Couture J (1997) Molecular biology of colorectal cancer. Curr Probl Cancer 21:233–300. doi:10.1016/S0147-0272(97)80003-7

    Article  CAS  PubMed  Google Scholar 

  9. Christensen JG, Goldworthy TL, Cattley RC (1999) Dysregulation of apoptosis by c-myc in transgenic hepatocytes and effects of growth factors and nongenotoxic carcinogens. Mol Carcinog 25:273–284. doi:10.1002/(SICI)1098-2744(199908)25:4<273::AID-MC6>3.0.CO;2-N

    Article  CAS  PubMed  Google Scholar 

  10. Finley GG, Schulz NT, Hill SA, Geiser JR, Pipas JM, Meisler AI (1989) Expression of myc gene family in different stages of human colorectal cancer. Oncogene 4:963–971

    CAS  PubMed  Google Scholar 

  11. Yander G, Halsey H, Kenna M, Augenlicht LH (1985) Amplification and elevated expression of c-myc in a chemically induced mouse colon tumour. Cancer Res 45:4433–4438

    CAS  PubMed  Google Scholar 

  12. Grandori C, Cowley SM, James LP, Eisenman RN (2000) The Myc/Max/Mad network and the transcriptional control of cell behavior. Annu Rev Cell Dev Biol 16:653–699. doi:10.1146/annurev.cellbio.16.1.653

    Article  CAS  PubMed  Google Scholar 

  13. Evan GI, Vousden KH (2001) Proliferation, cell cycle and apoptosis in cancer. Nature 411:342–348. doi:10.1038/35077213

    Article  CAS  PubMed  Google Scholar 

  14. Oster SK, Ho CS, Soucie EL, Penn LZ (2002) The myc oncogene: marvellously complex. Adv Cancer Res 84:81–154. doi:10.1016/S0065-230X(02)84004-0

    Article  CAS  PubMed  Google Scholar 

  15. Sano H, Kawahito Y, Wilder RL (1995) Expression of cyclooxygenase-1 and -2 I human colorectal cancer. Cancer Res 55:3785–3789

    CAS  PubMed  Google Scholar 

  16. Rao DR, Pulasani SR, Chawan CB (1986) Natural inhibitors of carcinogenesis: fermented milk products. In: Reddy BS, Cohen LA (eds) Diet, nutrition and cancer. A critical evaluation, vol 2. CRC press, Boca Raton, pp 63–75

    Google Scholar 

  17. Le MG, Moulton LH, Hill C, Krammer A (1986) Consumption of dairy produce and alcohol in a case control study of breast cancer. J Natl Cancer Inst 77:633–636

    CAS  PubMed  Google Scholar 

  18. Mitsuoka T (1990) Bifidobacteria and their role in human health. J Ind Microbiol 6:263–268. doi:10.1007/BF01575871

    Article  Google Scholar 

  19. Steinmetz KA, Porter JD (1991) A review of vegetables, fruits, and cancer. Epidemiol Cancer 2:325–357

    CAS  Google Scholar 

  20. Howe GR, Benito E, Castello R (1992) Dietary intake of fiber and decreased risk of cancers of the colon and rectum: evidence from the combined analysis of 13 case-control studies. J Natl Cancer Inst 84:1887–1896. doi:10.1093/jnci/84.24.1887

    Article  CAS  PubMed  Google Scholar 

  21. Kumar A, Singh NK, Sinha PR (2009) Inhibition of 1,2 dimethylhydrazine induced colon genotoxicity in rats by the administration of probiotic curd. Mol Biol Rep. doi:10.1007/s11033-009-9519-1

    Google Scholar 

  22. Arvind, Singh NK, Sinha PR, Kumar R (2008) Effect of Acidophilus-casei dahi (Probiotic Dahi) on lipids in 1,2 dimethylhydrazine induced intestinal cancer in rats. Int J Probiot Prebiot (in press)

  23. Singh NK, Arvind, Sinha PR (2007) Chemopreventive effect of Probiotic dahi (curd) containing Lactobacillus acidophilus and Lactobacillus casei on 1,2 dimethyhydrazine induced colon carcinogenesis in rats. Int J Probiot Prebiot 2(4):195–202

    Google Scholar 

  24. Kumar R, Sinha PR, Yadav BR, Arvind, Singh NK, Tonk R (2009) Effect of probiotic dahi (curd) on cytogenetic profile during 1,2-dimethyl hydrazine induced colon carcinogenesis in rats. Int J Probiot Prebiot 4(1):45–50

    Google Scholar 

  25. Vibha (2004) Effect of standard Lactobacilli on cardiovascular disease risk factors for their potential application as probiotics. Ph.D. Thesis. NDRI (Deemed University), Karnal, India

  26. AOAC (1984) Official methods of analysis. In: Association of official agric. chemists, vol. 877, 14th edn. Washington DC, p 988

  27. Rao CV, Rivenson A, Simi B, Zhang E (1995) Chemoprevention of colon carcinogenesis by sulindac, a non steroidal anti inflammatory agents. Cancer Res 55:1464–1472

    CAS  PubMed  Google Scholar 

  28. Tavan E, Cayuela C, Antoine J, Trugnan G (2002) Effects of dairy products on heterocyclic aromatic amine induced rat colon carcinogenesis. Carcinogenesis 23:477–483. doi:10.1093/carcin/23.3.477

    Article  CAS  PubMed  Google Scholar 

  29. Ahuja Y, Saran R (1999) Alkaline single cell gel electrophoresis assay. J Cytol Genet 34:57–62

    Google Scholar 

  30. Cereda H, Delincee H, Haine H, Rupp H (1997) The DNA “comet assay” as a rapid screening technique to control irradiated food. Mutat Res 375:167–181. doi:10.1016/S0027-5107(97)00012-2

    Google Scholar 

  31. Nadin SB, Vargas-Roig LM, Ciocca DR (2001) A silver staining method for single cell gel assay. J Histochem Cytochem 49:1183–1186

    CAS  PubMed  Google Scholar 

  32. Tao L, Kramer P, Wang W, Yang S, Lubet R, Steele V, Pereira M (2002) Altered expression of c-myc, p16and p27 in the rat colon tumours and its reversal by short-term treatment with chemopreventive agents. Carcinogenesis 23(9):1447–1454. doi:10.1093/carcin/23.9.1447

    Article  CAS  PubMed  Google Scholar 

  33. Badawi AF, EI-Sohemy A, Stephen LL, Ghoshal AK, Archer MC (1998) Effects of dietary n-3 and n-6 polyunsaturated fatty acids on the expression of cyclooxygenase-1 and -2 and p21 ras in rat mammary glands. Carcinogenesis 19:905–910. doi:10.1093/carcin/19.5.905

    Article  CAS  PubMed  Google Scholar 

  34. McIntosh G, Royle P, Playne M (1999) A probiotic strain of Lactobacillus acidophilus reduces DMH induced large intestinal tumours in male Sprague-Dawley Rats. Nutr Cancer 35(2):153–159. doi:10.1207/S15327914NC352_9

    Article  CAS  PubMed  Google Scholar 

  35. Singh J, Rivenson A, Tomita M, Shimamura S, Ishibashi N, Reddy BS (1997) Bifidobacterium longum, a lactic acid producing intestinal bacterium, inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Carcinogenesis 18(4):833–841. doi:10.1093/carcin/18.4.833

    Article  CAS  PubMed  Google Scholar 

  36. Rowland IR, Bearne CA, Fischer R, Pool-Zobel BL (1996) The effect of lactulose on DNA damage induced by DMH in the colon of human flora associated rats. Nutr Cancer 26(1):37–47

    Article  CAS  PubMed  Google Scholar 

  37. Reddy BS, Hirose Y, Cohen L, Simi B, Cooma I, Rao CV (2000) Preventive potential of wheat bran fraction against experimental colon carcinogenesis: implication for human colon cancer prevention. Cancer Res 60:4792–4797

    CAS  PubMed  Google Scholar 

  38. Rao CV, Kawamori T, Hamid R, Reddy BS (1999) Chemoprevention of colonic aberrant crypt foci by an inducible nitric oxide synthase-selective inhibitor. Carcinogenesis 20(4):641–644. doi:10.1093/carcin/20.4.641

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We acknowledge thanks to Indian council of agricultural research, India.

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Authors and Affiliations

  1. Animal Biochemistry Division, National Dairy Research Institute, Karnal, Haryana, 132001, India

    Arvind Kumar, Nikhlesh Kumar Singh, Pushpalata Rabindra Sinha & Raj Kumar

  2. 4984, Street No. 1, Near Old Poultry Farm, Shiv Colony, Karnal, Haryana, 132001, India

    Arvind Kumar

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Correspondence to Arvind Kumar.

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Kumar, A., Singh, N.K., Sinha, P.R. et al. Intervention of Acidophilus-casei dahi and Wheat bran against molecular alteration in colon carcinogenesis. Mol Biol Rep 37, 621–627 (2010). https://doi.org/10.1007/s11033-009-9649-5

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