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Association of goat (Capra hircus) CD4 gene exon 6 polymorphisms with ability of sperm internalizing exogenous DNA

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Abstract

As one of the transport systems on the sperm plasma membrane, CD4 molecule plays a distinct role in the process of sperm/DNA interaction. This makes it possible to explain the mechanism of sperm-mediated gene transfer (SMGT), which at present is still a mystery in this area. In this study, seminal samples of 60 individuals from seven breed bucks were collected to detect the ability of sperm in internalizing exogenous DNA, and genomic DNA from 147 individual blood samples (including 60 bucks referred ahead) were extracted to test the polymorphisms of CD4 genes by using PCR-SSCP technique. Then the correlation between them was evaluated. The results showed that: (1) it was a novel finding that breed-dependence of exogenous DNA binding to goat spermatozoa. There was the most significant difference among the buck breeds of sperm in binding exogenous DNA (F(6, 53) = 4.811, P = 0.001) and in internalizing them into nuclei (F(6, 53) = 4.587, P = 0.001). The ability of Lezhi Black goat was the lowest (P < 0.01) among the seven breeds. (2) There was no significant correlation between the ability of sperm in internalizing exogenous DNA and each semen quality parameter (P > 0.05). (3) In particular, three single-nucleotide polymorphisms (SNP) were described and there was one SNP (G/A700) of CD4 gene that made G234R substitution in the amino acid sequence of CD4 molecule. Nanjiang Yellow goat and Lezhi Black goat had higher hereditary variation compared with other breeds. (4) CD4 polymorphisms were highly associated with the ability of sperm in internalizing exogenous DNA. The SNP of Caprine CD4 gene exon 6 might be an important molecular marker of the ability to internalize exogenous DNA into sperm.

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Acknowledgments

The work was supported by: The national natural science foundation of China, grant number: 30600430, National science and technology specific project, grant number: 2008ZX08008-004). We thank Dr. Juan Li (Department of Genetics and Biotechnology, Research Centre Foulum, Aarhus University, DK-8830 Tjele, Denmark) for critical reading of the manuscript, Mr. Hongding Gao (College of Animal Science and Technology, China Agricultural University, Beijing 100094, China) for his help in statistical analysis and the herd owners for their cooperation. We also thank the anonymous reviewers for constructive and professional comments.

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Authors and Affiliations

  1. College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Southwest University, Beibei, Chongqing, 400716, People’s Republic of China

    Lingbin Wang, Jingsheng Fan, Mingju Yu, Shuangyan Zheng & Yongju Zhao

  2. Sichuan Animal Science Academy, Chengdu, Sichuan, 610066, People’s Republic of China

    Jingsheng Fan

  3. Department of Laboratory Animal Science, Medical College of Nanchang University, Nanchang, 330006, People’s Republic of China

    Shuangyan Zheng

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  1. Lingbin Wang
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  2. Jingsheng Fan
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  3. Mingju Yu
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Correspondence to Yongju Zhao.

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Lingbin Wang, Jingsheng Fan contributed equally to this work.

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Wang, L., Fan, J., Yu, M. et al. Association of goat (Capra hircus) CD4 gene exon 6 polymorphisms with ability of sperm internalizing exogenous DNA. Mol Biol Rep 38, 1621–1628 (2011). https://doi.org/10.1007/s11033-010-0272-2

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  • DOI: https://doi.org/10.1007/s11033-010-0272-2

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