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Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino

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Abstract

This study reports the effect of explant type and plant growth regulators (PGRs) on callus induction in Cnidium officinale. Compared to stem, root explant showed maximum percent callus formation of 75% on Murashige and Skoog (MS) medium containing 2.3 µM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.2 µM benzyladenine (BA). At 30th day of callus culture on the said medium, callus fresh weight was sevenfold higher than other tested PGRs treatments. It was noted that MS medium supplemented with 27.1 µM 2,4-D showed the highest 0.30 mg g−1 DW of total phenols, while total flavonoids content reached to a maximum of 0.05 mg g−1 DW on the MS medium supplemented with 4.5 µM 2,4-D and 2.2 µM BA. Conversely, maximum (83.9%) DPPH free radical scavenging activity was observed in calli grown on the MS medium supplemented with 2.3 µM 2,4-D and 2.2 µM BA. The high-performance liquid chromatography (HPLC) analysis revealed higher phthalide content in callus than intact roots of in vitro plants. While 3-butylidenephthalide content in callus was comparable to the intact shoots and roots of in vitro grown C. officinale. The concentrations of 2,4-D played a significant role in the production of phthalide and 3-butylidenephthalide. Additional measures are recommended to further enhance their production in vitro.

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Acknowledgements

Xiuxia Ren, Dong Il Kang and Luc The Thi were supported by a scholarship from the BK21 Plus Program, the Ministry of Education, Republic of Korea. The support of the Korea Institute of Planning and Evaluation for technology in Food, Agriculture, Forestry and Fisheries (Project No.116057-03) is highly acknowledged.

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Authors and Affiliations

  1. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, South Korea

    Muhammad Adil & Byoung Ryong Jeong

  2. Division of Applied Life Science Graduate School (BK 21 Plus Program), Department of Horticulture, Gyeongsang National University, Jinju, 52828, South Korea

    Xiuxia Ren, Luc The Thi & Byoung Ryong Jeong

  3. Research Institute of Life Science, Gyeongsang National University, Jinju, 52828, South Korea

    Dong Il Kang & Byoung Ryong Jeong

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  1. Muhammad Adil
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  2. Xiuxia Ren
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Adil, M., Ren, X., Kang, D.I. et al. Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino. Mol Biol Rep 45, 1919–1927 (2018). https://doi.org/10.1007/s11033-018-4340-3

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  • DOI: https://doi.org/10.1007/s11033-018-4340-3

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