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Regulated expression of Gemin5, Xrn1, Cpeb and Stau1 in the uterus and ovaries after superovulation and the effect of exogenous estradiol and leptin in rodents

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Abstract

The aim of this study was to evaluate whether Gemin 5, Cpeb, Xrn1, and Stau1 expression in rodent ovaries and uterine tissues is dependent on gonadotropins, steroid hormones, and leptin in the superovulation and ovariectomized mouse models of menopause. Treatment of pregnant mare serum gonadotropin-primed rats with human chorionic gonadotropin (hCG) significantly induced Stau1 and Gemin 5 messenger RNA expression in rat ovaries. Gemin 5 expression in ovaries was sustained at relatively high levels at 12 h and 24 h post hCG treatment compared to Stau1, suggesting its role in follicle development, ovulation, and luteogenesis in rat ovaries. Induced expression of Stau1 and Gemin 5 in the uterine tissue post hCG treatment at 12 h and 24 h—the duration between ovulation and post-ovulation—suggests their regulation by hCG and/or ovarian steroids, which are required for pregnancy establishment and maintenance. Cpeb expression was significantly higher (p < 0.05) in the uterine tissues after combined treatment of estradiol and leptin at 4 h. Further, the significant upregulation of uterine Gemin 5 and Xrn1 by the synergistic activities of leptin and estradiol at 40 h in ovariectomized mice establishes them as targets of cross-talk. Although these are preliminary data, the combination of Gemin 5, Cpeb, Xrn1, and Stau1 transcript alterations in rodent ovaries and uterine tissue displayed in two different experimental models underscore their importance as therapeutic targets for anovulation or in overcoming endometrial homeostasis disturbances during pregnancy due to obesity.

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Acknowledgements

PSS acknowledges University Grants Commission, India for startup-grant, Department of Science and Technology Science and Engineering Research Board, India (DST-SERB) (YSS/2014/000020). We are also grateful to the Department of Science and Technology-Fund for Improvement of Science and Technology (DST-FIST) and University Grants Commission Special assistance program for providing infrastructure support to the Department of Biosciences, Mangalore University. We are grateful to Department of Science and Technology (DST)-Promotion of University Research and Scientific Excellence (PURSE), Mangalore University, we acknowledge Sharmila KS for the technical support. Padmanaban S Suresh was affiliated with Mangalore University when work was carried out and thank the support of Mangalore University.

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Authors and Affiliations

  1. Department of Biosciences, Mangalore University, Mangalagangothri, Mangalore, Karnataka, 574 199, India

    Abhishek Shetty

  2. Department of Biochemistry and Molecular Biology, Central University of Kerala, Kasargod, Kerala, 671 316, India

    Thejaswini Venkatesh

  3. Department of Nutrition and Metabolism, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15, Kuramoto-cho, Tokushima City, 770-8503, Japan

    Rie Tsutsumi

  4. School of Biotechnology, National Institute of Technology, Calicut, Kerala, 673 601, India

    Padmanaban S. Suresh

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  1. Abhishek Shetty
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Correspondence to Padmanaban S. Suresh.

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Shetty, A., Venkatesh, T., Tsutsumi, R. et al. Regulated expression of Gemin5, Xrn1, Cpeb and Stau1 in the uterus and ovaries after superovulation and the effect of exogenous estradiol and leptin in rodents. Mol Biol Rep 46, 2533–2540 (2019). https://doi.org/10.1007/s11033-019-04606-z

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  • DOI: https://doi.org/10.1007/s11033-019-04606-z

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