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Phenolic compounds, antioxidant capacity and antimutagenic activity in different growth stages of in vitro raised plants of Origanum vulgare L.

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Abstract

Efficient micropropagation procedure was developed for Origanum vulgare, a high-value culinary herb, and the phytochemicals, phenolic content, antioxidant and antimutagenic activity of leaf and stem, derived from different growing stages were analyzed. The agar solidified Murashige and Skoog (MS) medium supplemented with a combination of 6-benzylaminopurine and α-naphthaleneacetic acid was optimized as best shoot-multiplication-medium. Shoots were rooted best on 1/2 strength MS medium supplemented with 50 µM indole-3-butyric acid (IBA). The plantlets were successfully acclimatized ex vitro in a soil, sand and farmyard manure mixture (2:1:1 v/v/v) with 100% survival rate in greenhouse. The total anthocyanin and total phenolic content were observed significantly higher in leaves of in vitro-raised plants. However, total tannin, flavonoid and antioxidant activity remained higher in leaves of mother plant maintained under ployhouse condition. All the plant extracts have shown significant antimutagenic activity except in vitro-growing plants. A total of 13 polyphenolic compounds were detected in different extracts using high performance liquid chromatography. Among these, catechin was detected maximum in in vitro-growing cultures and chlorogenic acid in leaves of mother plant. These findings will help the farmers, medicinal plant growers, and industries for mass multiplication and effective extraction of phytochemicals from O. vulgare.

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Abbreviations

AAE:

Ascorbic acid equivalent

ABTS:

2,2-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid)

asl:

Above mean sea level

BAP:

6-Benzylaminopurine

CN:

Cyanidin 3-glucoside

DPPH:

2,2-Diphenyl-1-picryhydrazyl

DAD:

Diode-array detection

GA3 :

Gibberellic acid

GAE:

Gallic acid equivalent

HPLC:

High performance liquid chromatography

IBA:

Indole-3-butyric acid

IL:

In vitro-raised plant leaf

IS:

In vitro-raised plant stem

IVG:

In vitro-growing cultures

MPL:

Mother plant leaf

MPS:

Mother plant stem

MS:

Murashige and Skoog

NAA:

α-Naphthaleneacetic acid

PBS:

Phosphate-buffered saline

PGRs:

Plant growth regulators

TAE:

Tannic acid equivalent

TBE:

Tris borate ethylenediaminetetraacetic acid

QE:

Quercetin equivalent

µM:

Micro mole

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Acknowledgements

Authors thank Director G. B. Pant National Institute of Himalayan Environment and Sustainable Development, for his encouragement and facilities. Authors also thank Head, Department of Biotechnology, Bhimtal Campus, Kumaun University Nainital for facilities and encouragement during the initial stage of experimentation. Colleagues of Biodiversity Conservation and Management theme are thanked for cooperation and help during the study. Anonymous reviewers are gratefully acknowledged for providing useful inputs to improve the manuscript draft.

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Authors and Affiliations

  1. G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand, 263643, India

    Aseesh Pandey, Tarun Belwal, I. D. Bhatt & R. S. Rawal

  2. Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand, 263136, India

    Aseesh Pandey & Sushma Tamta

  3. G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Sikkim Regional Centre, Gangtok, East Sikkim, 737101, India

    Aseesh Pandey

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  1. Aseesh Pandey
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Pandey, A., Belwal, T., Tamta, S. et al. Phenolic compounds, antioxidant capacity and antimutagenic activity in different growth stages of in vitro raised plants of Origanum vulgare L.. Mol Biol Rep 46, 2231–2241 (2019). https://doi.org/10.1007/s11033-019-04678-x

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