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Antioxidative enzymes and isozymes analysis of taro genotypes and their implications in Phytophthora blight disease resistance

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Abstract

Assessment of the differential expression of antioxidative enzymes and their isozymes, was done in 30 day-old ex vitro raised plants of three highly resistant (DP-25, Jhankri and Duradim) and one highly susceptible (N-118) genotypes of taro [Colocasia esculenta (L.) Schott]. Antioxidative enzymes were assayed in the ex vitro plants, 7 days after inoculation with the spores (15,000 spores ml−1 water) of Phytophthora colocasiae Raciborski to induce taro leaf blight disease. Uninoculated ex vitro plants in each genotype were used as control. The activity of superoxide dismutase (SOD) and guaiacol peroxidase (GPX) increased under induced blight condition when compared with control. Increase in antioxidative enzymes was more (67–92%) in the resistant genotypes than that (21–29%) of the susceptible genotype. The zymograms of SOD and GPX in the resistant genotypes, with pathogenic infection, showed increased activity for anodal isoform of SOD and increased expression and/or induction of either POX 1 or POX 2 isoforms of GPX. In susceptible genotype, expression of the above isoforms was faint for SOD and nearly absent for GPX under both blight free and induced blight conditions. Induction and/or increased activity of particular isoform of SOD and GPX against infection of Phytophthora colocasiae in the resistant genotypes studied led to the apparent conclusion of linkage of isozyme expression with blight resistance in taro. This might be an important criterion in breeding of taro for Phytophthora leaf blight resistance.

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Abbreviations

EDTA:

Ethylene diamine tetra acetic acid

GPX:

Guaiacol peroxidase

NBT:

Nitroblue tetrazolium

PAGE:

Polyacrylamide gel electrophoresis

POX:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TEMED:

N,N,N′,N′-tetramethylethylene-diamine

TLB:

Taro leaf blight

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Acknowledgements

The first author gratefully acknow- ledges the financial support of ICAR NATP-CGP to carry out the present investigation. The authors are also thankful to the Director, Central Tuber Crops Research Institute, Trivandrum, India and Head, Regional Centre of Central Tuber Crops Research Institute, Bhubaneswar, India for infrastructure facilities.

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Authors and Affiliations

  1. Department of Crop Improvement, Horticulture and Agricultural Botany, Institute of Agriculture, Visva-Bharati, Sriniketan, 731236, West Bengal, India

    Manas Ranjan Sahoo, Paresh C. Kole & Jayant S. Bhat

  2. Centre for Cellular and Molecular Biology (CSIR), Hyderabad, 500 007, Andhra Pradesh, India

    Madhumita DasGupta

  3. Regional Centre of Central Tuber Crops Research Institute, Dumduma Housing Board, Bhubaneswar, 751019, Orissa, India

    Archana Mukherjee

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  1. Manas Ranjan Sahoo
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  2. Madhumita DasGupta
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Correspondence to Madhumita DasGupta.

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Sahoo, M.R., DasGupta, M., Kole, P.C. et al. Antioxidative enzymes and isozymes analysis of taro genotypes and their implications in Phytophthora blight disease resistance. Mycopathologia 163, 241–248 (2007). https://doi.org/10.1007/s11046-007-9000-4

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