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Fusarium verticillioides: Evaluation of Fumonisin Production and Effect of Fungicides on In Vitro Inhibition of Mycelial Growth

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Abstract

The objectives of this study were to evaluate the fumonisin production by 16 F. verticillioides strains on corn cultures and the effect of quintozene and fludioxonil + metalaxyl-M fungicides on “in vitro” mycelial growth on agar. In addition, the effect of fludioxonil + metalaxyl-M on fumonisin production in defined liquid culture medium was analyzed. Fumonisin B1 levels on corn cultures ranged from 2.41 to 3996.36 μg/g and the F. verticillioides 103F strain produced the highest level (3996.36 ± 390.49 μg/g, P < 0.05). F. verticillioides strains were inoculated in potato dextrose agar with the addition of quintozene (75 to 9,375 μg/ml) and fludioxonil + metalaxyl-M (1.5 + 0.6 to 187.5 + 75 μg/ml) in order to evaluate the effect of these fungicides on “in vitro” mycelial growth. The F. verticillioides strains showed great variability concerning ED50 values, which were below the recommended application dose for quintozene, but above that for fludioxonil + metalaxyl-M. Moreover, fungicide addition to the culture medium increased mean FB1 levels compared to the control, suggesting the importance of focusing on the effect of fungicides on mycotoxin production as well as on the phytopathogen control.

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Acknowledgments

The authors thank the CNPq (the Brazilian Government Organisation for grant aid and fellowship to Brazilian researchers), CNPq/Ministry of Agriculture, the Araucária Foundation, PPSUS/Brazilian Ministry of Health, Paraná Fund/SETI and CAPES (Co-ordination for Formation of High Level Professionals) for the financial support. The authors also thank the CNPq for the productivity fellowship granted to E.Y.S. Ono, E.Y. Hirooka and M.A. Ono.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Center of Exact Sciences, State University of Londrina, P.O. Box 6001, Londrina, Paraná, 86051-980, Brazil

    Virgínia Carla A. Falcão, Tatiana de Ávila Miguel & Elisabete Yurie Sataque Ono

  2. Department of Pathological Sciences, State University of Londrina, P.O. Box 6001, Londrina, Paraná, 86051-980, Brazil

    Mario Augusto Ono

  3. Department of Applied Mathematics, State University of Londrina, P.O. Box 6001, Londrina, Paraná, 86051-980, Brazil

    Edio Vizoni

  4. Department of Food Science and Technology, State University of Londrina, P.O. Box 6001, Londrina, Paraná, 86051-980, Brazil

    Elisa Yoko Hirooka

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  1. Virgínia Carla A. Falcão
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Correspondence to Elisabete Yurie Sataque Ono.

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Falcão, V.C.A., Ono, M.A., de Ávila Miguel, T. et al. Fusarium verticillioides: Evaluation of Fumonisin Production and Effect of Fungicides on In Vitro Inhibition of Mycelial Growth. Mycopathologia 171, 77–84 (2011). https://doi.org/10.1007/s11046-010-9339-9

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