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Trichophyton indotineae sp. nov.: A New Highly Terbinafine-Resistant Anthropophilic Dermatophyte Species

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Abstract

In this report, we describe the first isolation of two highly terbinafine (TRF)-resistant Trichophyton interdigitale-like strains from a Nepali patient and an Indian patient with tinea corporis in Japan. These strains (designated NUBS19006 and NUBS19007) exhibited a TRF minimal inhibitory concentration (MIC) of > 32 mg/L and contained a missense mutation (Phe397Leu) in squalene epoxidase (SQLE) gene. The internal transcribed spacer (ITS) region sequences amplified from the isolates (NUBS19006 and NUBS19007) were 99.5% identical to Japanese isolates of T. interdigitale and T. interdigitale strain CBS 428.63. The homology of region sequences were also 97.6% identical to T. mentagrophytes strain CBS 318.56. Moreover, the ITS sequences amplified from the isolates were 100% identical to highly TRF-resistant strains of T. interdigitale, which were isolated in Delhi, India, and harbored mutations in SQLE. The urease test on Christensen’s urease agar was positive for T. mentagrophytes and T. interdigitale after 7 days of incubation. On the other hand, the type strain of T. rubrum CBS 100081 T and highly TRF-resistant strains (NUBS19006 and NUBS19007) were negative on Christensen urease agar after 7 and 14 days of incubation. Moreover, NUBS19006 and NUBS19007 were also negative reaction on the hair perforation test. To avoid confusion in the taxonomy of the T. mentagrophytes/T. interdigitale complex, we suggest that the highly TRF-resistant Indian strains be considered a new species independent of T. interdigitale, according to clinical and mycological features.

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Acknowledgements

We thanks to Dr. Atsuhiko Hasegawa, who advised to us for mycological identifications.

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Authors and Affiliations

  1. Department of Veterinary Dermatology, Nihon University College of Bioresource Sciences, 1866 Kameino, Fujisawa, Kanagawa, 252-0880, Japan

    Rui Kano & Hiroshi Kamata

  2. Department of Dermatology, Juntendo University Urayasu Hospital, 2-1-1 Tomioka, Urayasu, Chiba, 279-0021, Japan

    Utako Kimura & Yasushi Suga

  3. Kakurai Dermatology Clinic, 905-1, Ojima, Shimotsuma, Ibaraki, 304-0051, Japan

    Maki Kakurai

  4. Department of Dermatology, Tokyo Medical University, 6-1-1 Nishishinjuku, Shinjuku-ku, Tokyo, 160-0023, Japan

    Junichiro Hiruma & Kazutoshi Harada

Authors
  1. Rui Kano
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  2. Utako Kimura
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  3. Maki Kakurai
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  4. Junichiro Hiruma
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  5. Hiroshi Kamata
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  6. Yasushi Suga
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  7. Kazutoshi Harada
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Correspondence to Rui Kano.

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Kano, R., Kimura, U., Kakurai, M. et al. Trichophyton indotineae sp. nov.: A New Highly Terbinafine-Resistant Anthropophilic Dermatophyte Species. Mycopathologia 185, 947–958 (2020). https://doi.org/10.1007/s11046-020-00455-8

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  • DOI: https://doi.org/10.1007/s11046-020-00455-8

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