Abstract
Biosynthesis of copper, zero-valent iron (ZVI), and silver nanoparticles using leaf extract of Dodonaea viscosa has been investigated in this report. There are no additional surfactants/polymers used as capping or reducing agents for these syntheses. The synthesized nanoparticles were characterized by UV–Vis spectroscopy, X-ray diffraction, atomic force microscopy, and high-resolution transmission electron microscopy. The phase analysis was performed using selected area electron diffraction. The pH dependence of surface plasmon resonance and subsequent size variation has been determined. The synthesized nanoparticles showed spherical morphology and the average size of 29, 27, and 16 nm for Cu, ZVI, and Ag nanoparticles, respectively. Finally, biosynthesized Cu, ZVI, and Ag nanoparticles were tested against human pathogens viz. Gram-negative Escherichia coli, Klebsiella pneumonia, Pseudomonas fluorescens and Gram-positive Staphylococcus aureus and Bacillus subtilis, and showed good antimicrobial activity.
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S. C. G. Kiruba Daniel and G. Vinothini contributed equally to this study.
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Fig. S1
UV-Visible spectra of copper nanoparticles synthesized with different amounts of Dodonaea viscosa leaf extract at pH 10 (A) 1 ml (B) 2 ml (C) 3 ml (D) 4 ml (E) 5 ml
Fig. S2
UV- Visible spectra of Zero Valent Iron nanoparticles synthesized from 10 mM FeCl3 with different amount of Dodonaea viscosa extract at room temperature
Fig. S3
UV-Visible spectra of Ag Nps synthesized using different amounts of Dodonaea viscosa leaf extract after 5 h of reaction (A) 250 µl (B) 500 µl (C) 1 ml (D) 2 ml extract with 1 mM AgNO3
Fig. S4
UV-Visible spectra of copper nanoparticles synthesized using 5 ml of D. viscosa extract at different pH (1) pH 6, (2) pH 8 and (3) pH 10 with 5 ml 1 mM CuCl2 at 50˚C
Fig. S5
UV-Visible spectra of Ag Nps synthesized using 2 ml of D. viscosa leaf extract with 1 mM AgNO3 at different pH from 2 to 10
Fig. S6
HRTEM images of Cu NP (A&B), ZVI NP (C&D) and Ag NP (E&F) taken at 10 and 50 nm as scale. (DOCX 6181 kb)
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Kiruba Daniel, S.C.G., Vinothini, G., Subramanian, N. et al. Biosynthesis of Cu, ZVI, and Ag nanoparticles using Dodonaea viscosa extract for antibacterial activity against human pathogens. J Nanopart Res 15, 1319 (2013). https://doi.org/10.1007/s11051-012-1319-1
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DOI: https://doi.org/10.1007/s11051-012-1319-1