Iba-1 protein is a widely recognized marker for microglial cells and was previously observed by the authors in microgliocyte nuclei. The aim of the present work was to refine these data using immunocytochemistry and confocal laser microscopy methods. Studies were performed on human brain fragments (cortex, striatum, substantia nigra, red nucleus) (n = 18, age 25–78 years). The results showed that Iba-1 protein accumulates in one or more areas in microgliocyte nuclei, which were neither the nucleolus nor clumps of heterochromatin. The causes for this are unclear. Iba-1 protein, apart from carrying out its main function (a role in phagocytosis), may also operate as a transcription factor.
Similar content being viewed by others
References
D. E. Korzhevskii and O. V. Kirik, “Brain microglia and microglial markers,” Morfologiya, 147, No. 3, 37–44 (2015).
D. E. Korzhevskii, O. V. Kirik, E. G. Sukhorukova, et al., Molecular Morphology. Fluorescence and Confocal Laser Microscopy Methods, SpetsLit, St. Petersburg (2014).
D. E. Korzhevskii, O. V. Kirik, E. G. Sukhorukova, and M. A. Syrtsova, “Microglia of the substantia nigra in the human brain,” Med. Akad. Zh., 14, No. 4, 68–72 (2014).
Y. Imai, I. Ibata, D. Ito, et al., “A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage,” Biochem. Biophys. Res. Commun., 224, 855–862 (1996).
L. A. Shapiro, Z. D. Perez, M. L. Foresti, et al., “Morphological and ultrastructural features of Iba1-immunolabeled microglial cells in the hippocampal dentate gyrus,” Brain Res., 1266, 29–36 (2009).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Morfologiya, Vol. 149, No. 2, pp. 73–76, March–April, 2016.
Rights and permissions
About this article
Cite this article
Korzhevskii, D.E., Kirik, O.V., Alekseeva, O.S. et al. Intranuclear Accumulation of Iba-1 Protein in Microgliocytes in the Human Brain. Neurosci Behav Physi 47, 435–437 (2017). https://doi.org/10.1007/s11055-017-0417-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-017-0417-z