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Micropropagation of Salvadora persica-a tree of arid horticulture and forestry

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Abstract

A micropropagation method for Jaal (Salvadora persica)—a tree of arid horticulture and forestry has been developed. Nodal segments of fresh shoot sprouts originated from axillary buds obtained from a plant around 35–40 years old lopped plant were used as explants for establishment of in vitro cultures. Surface-sterilized explants produced optimum number of shoots through activation of axillary buds on Murashige and Skoog’s (MS) medium containing 8.88 μM BA (6-benzyladenine) + additives (25 mgl−1 each of adenine sulphate, arginine, citric acid, 50 mgl−1 ascorbic acid). The shoot multiplication was influenced by the successive transfer of the mother explants for 4–5 passages. The maximum number (23.1 ± 0.73 shoots per explant) of shoots were regenerated on MS supplemented with 1.11 μM BA + 1.16 μM Kn (Kinetin) + 0.54 μM NAA (α-naphthalene acetic acid). About 90% shoots pulse-treated with a combination of 2460.27 μM Indole-3-butyric acid (IBA) + 494.56 μM NOA (2-naphthoxy acetic acid) were rooted ex vitro on soilrite within 15–18 days. Over 80% cloned plantlets were hardened successfully in a green house and transferred to polybag/pots.

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Acknowledgments

The author (M.P.) wishes to acknowledge the support of the University Grants Commission (UGC), New Delhi for the award of Post Doctoral Fellowship. Financial assistance provided by Council of Scientific & Industrial Research (CSIR), New Delhi, to the authors (K.R. and P.G.) is gratefully acknowledged. We also thank Dr. (Mrs.) Sudhi Rajiv, Professor of English, J.N. Vyas University, Jodhpur for language improvement of this revised manuscript.

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Authors and Affiliations

  1. Biotechnology Unit, Jai Narain Vyas University, Jodhpur, 342033, India

    M. Phulwaria, Kheta Ram, Priyanka Gahlot & N. S. Shekhawat

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  1. M. Phulwaria
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  2. Kheta Ram
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  3. Priyanka Gahlot
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  4. N. S. Shekhawat
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Correspondence to M. Phulwaria.

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Phulwaria, M., Ram, K., Gahlot, P. et al. Micropropagation of Salvadora persica-a tree of arid horticulture and forestry. New Forests 42, 317–327 (2011). https://doi.org/10.1007/s11056-011-9254-z

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  • DOI: https://doi.org/10.1007/s11056-011-9254-z

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