Photosynthetica 2013, 51(1):151-157 | DOI: 10.1007/s11099-013-0007-4

Chlorophyll content and photosystem II efficiency in soybean exposed to supplemental ultraviolet-B radiation

Z. Hu1,2,*, H. Li1, S. Chen1, Y. Yang1
1 Yale-NUIST Center on Atmospheric Environment & School of Environmental Science and Engineering, Nanjing University of Information Science & Technology, Nanjing, China
2 Key Laboratory of Agrometeorological Safeguard and Applied Technique, China Meteorological Administration, Zhengzhou, China

Chlorophyll (Chl) a fluorescence parameters and rapid light curves of soybean [Glycine max (L.) Merrill] were measured by pulse amplitude modulation fluorometry. Measurements were taken during different stages of soybean growth under field conditions with 20% enhancement in ultraviolet-B (UV-B) radiation. Results showed that supplemental UV-B radiation decreased Chl contents by 5.5% (P=0.048), 8.7% (P=0.046), and 10.5% (P=0.005) in seedling, in branching-flowering, and in pod-setting stages, respectively. In the branching-flowering and pod-setting stages, maximum quantum yield of photosystem (PS) II photochemistry (Fv/Fm) decreased by 6.1% (P=0.001) and 3.0% (P=0.009), respectively. Supplemental UV-B radiation significantly decreased the effective quantum yield (Y). The photosynthetic capacity at light saturation (Pm) also decreased in both the seedling and branching-flowering stages by 28.9% (P=0.007) and 15.5% (P=0.041), respectively. However, Y and Pm showed no significant difference in the trefoil and pod-setting stages with and without the UV treatment. The light saturation parameter (E k) decreased by 21.1% (P=0.000) and 23.2% (P=0.029) in the trefoil and seedling stages, respectively. Moreover, the initial slope (α) decreased by 21.1% (P=0.001) in the branching-flowering stage. Nonphotochemical quenching (NPQ) in the seedling stage and photochemical quenching coefficient (qp) in the branching-flowering stage decreased significantly under UV-B treatments. The results of the present study suggest that supplemental UV-B radiation adversely affected Chl content and electron transport activity in PSII and consequently decreased the photosynthetic efficiency of soybean plants.

Additional key words: chlorophyll content; fluorescence quenching; Glycine max; rapid light curves; ultraviolet-B radiation

Received: June 24, 2011; Accepted: November 16, 2012; Published: March 1, 2013  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Hu, Z., Li, H., Chen, S., & Yang, Y. (2013). Chlorophyll content and photosystem II efficiency in soybean exposed to supplemental ultraviolet-B radiation. Photosynthetica, 51(1), 151-157. doi: 10.1007/s11099-013-0007-4
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Agrawal, S.B., Rathore, D., Singh, A.: Combined effects of enhanced ultraviolet-B radiation and mineral nutrients on growth, biomass accumulation and yield characteristics of two cultivars of Vigna radiata L. - J. Environ. Biol. 27: 55-60, 2006.
    2. Azevedo Neto, A.D., Prisco, J.T., Gomes-Filho, E.: Changes in soluble amino-N, soluble proteins and free amino acids in leaves and roots of salt-stressed maize genotypes. - J. Plant Interact. 4: 137-144, 2009. Go to original source...
    3. Baker, N.R.: Chlorophyll fluorescence: a probe of photosynthesis in vivo. - Annu. Rev. Plant Biol. 59: 89-113, 2008. Go to original source...
    4. Belshe, E.F., Durako, M.J., Blum, J.E.: Photosynthetic rapid light curves (RLC) of Thalassia testudinum exhibit diurnal variation. - J. Exp. Mar. Biol. Ecol. 342: 253-268, 2007. Go to original source...
    5. Björkman, O., Demming, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins. - Planta 170: 489-504, 1987. Go to original source...
    6. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    7. Brandle, J.R., Campbell, W.F., Sisson, W.B., Caldwell, M.M.: Net photosynthesis, electron transport capacity, and ultrastructure of Pisum sativum L. exposed to ultraviolet-B radiation. - Plant Physiol. 60: 165-169, 1977. Go to original source...
    8. Cechin, I., Fumis, T.deF., Dokkedal, A.L.: Growth and physiological response of sunflower plants exposed to ultraviolet-B radiation. - Ciênc. Rural 37: 85-90, 2007. Go to original source...
    9. Correia, C.M., Pereira, J.M.M., Coutinho, J.F. et al.: Ultraviolet-B radiation and nitrogen affect the photosynthesis of maize: a Mediterranean field study. - Eur. J. Agron. 22: 337-347, 2005. Go to original source...
    10. Cruz, S., Serôdio, J.: Relationship of rapid light curves of variable fluorescence to photoacclimation and non-photochemical quenching in a benthic diatom. - Aquat. Bot. 88: 256-264, 2008. Go to original source...
    11. Day, T.A., Vogelmann, T.C.: Alterations in photosynthesis and pigment distributions in pea leaves following UV-B exposure. - Physiol. Plant. 94: 433-440, 1995. Go to original source...
    12. Erickson, D.J., III, Zepp, R.G., Atlas, E.: Ozone depletion and the air-sea exchange of greenhouse and climate reactive gases. - Chemosphere-Glob. Change Sci. 2: 137-149, 2000. Go to original source...
    13. Fedina, I., Hidema, J., Velitchkova, M., Georgieva, K., Nedeva, D.: UV-B induced stress responses in three rice cultivars. - Biol. Plant. 54: 571-574, 2010. Go to original source...
    14. Feng, H., Li, S., Xue, L. et al.: The interactive effects of enhanced UV-B radiation and soil drought on spring wheat. - South Afr. J. Bot. 73: 429-434, 2007. Go to original source...
    15. Gao, W., Zheng, Y.F., Slusser, J.R. et al.: Effects of supplementary ultraviolet-B irradiance on maize yield and qualities: A field experiment. - Photochem. Photobiol. 80: 127-131, 2004. Go to original source...
    16. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta 990: 87-92, 1989. Go to original source...
    17. He, J.M., She, X.P., Meng, Z.N., Zhao, W.M.: Reduction of rubisco amount by UV-B radiation is related to increased H2O2 content in leaves of mung bean seedlings. - J. Plant Physiol. Mol. Biol. 30: 291-296, 2004.
    18. Kakani, V.G., Reddy, K.R., Zhao, D., Gao, W.: Senescence and hyperspectral reflectance of cotton leaves exposed to ultraviolet-B radiation and carbon dioxide. - Physiol. Plant. 121: 250-257, 2004. Go to original source...
    19. Karpinski, S., Escobar, C., Karpinski, B. et al.: Photosynthetic electron transport regulates the expression of cytosolic ascorbate peroxidase genes in Arabidopsis during excess light stress. - Plant Cell 9: 627-640, 1997. Go to original source...
    20. Liang, C.J., Zhang, G.S., Zhou, Q.: Effect of Cerium on photosynthetic pigments and photchemical reaction activity in soybean seedling under ultraviolet-B radiation stress. - Biol. Trace Elem. Res. 142: 796-806, 2011. Go to original source...
    21. Marenco, R.A., Antezana-Vera, S.A., Nascimento, H.C.S.: Relationship between specific leaf area, leaf thickness, leaf water content and SPAD-502 readings in six Amazonian tree species. - Photosynthetica 47: 184-190, 2009. Go to original source...
    22. Markwell, J., Osterman, J.C., Mitchell, J.L.: Calibration of the Minolta SPAD-502 leaf chlorophyll meter. - Photosynth. Res. 46: 467-472, 1995. Go to original source...
    23. Mielke, M.S., Schaffer, B., Li, C.: Use of a SPAD meter to estimate chlorophyll content in Eugenia uniflora L. leaves as affected by contrasting light environments and soil flooding. - Photosynthetica 48: 332-338, 2010. Go to original source...
    24. Natali, S.M., Sañudo-Wilhelmy, S.A., Lerdau, M.T.: Effects of elevated carbon dioxide and nitrogen fertilization on nitrate reductase activity in sweetgum and loblolly pine trees in two temperate forests. - Plant Soil 314: 197-210, 2009. Go to original source...
    25. Paknejad, F., Nasri, M., Moghadam, H. et al.: Effects of drought stress on chlorophyll fluorescence parameters, chlorophyll content and grain yield of wheat cultivars. - J. Biol. Sci. 7: 841-847, 2007. Go to original source...
    26. Pandey, J., Chaplot, K.: Effects of enhanced UV-B radiation on physiological and biochemical characteristics of wheat. - Res. Crops 8: 401-405, 2007.
    27. Papageorgiou, G.C., Govindjee,: Photosystem II fluorescence: Slow changes - scaling from the past. - J. Photochem. Photobiol. B: Biol. 104: 258-270, 2011. Go to original source...
    28. Pradhan, M.K., Nayak, L., Joshi, P.N. et al.: Developmental phase-dependent photosynthetic responses to ultraviolet-B radiation: damage, defence, and adaptation of primary leaves of wheat seedlings. - Photosynthetica 46: 370-377, 2008. Go to original source...
    29. Ralph, P.J., Gademann, R.: Rapid light curves: A powerful tool to assess photosynthetic activity. - Aquat. Bot. 82: 222-237, 2005. Go to original source...
    30. Ranjbarfordoei, A., Samson, R., van Damme, P.: Photosynthesis performance in sweet almond [Prunus dulcis (Mill) D. Webb] exposed to supplemental UV-B radiation. - Photosynthetica 49: 107-111, 2011. Go to original source...
    31. Rascher, U., Liebig, M., Luttge, U.: Evaluation of instant light-response curves of chlorophyll fluorescence parameters obtained with a portable chlorophyll fluorometer on site in the field. - Plant Cell Environ. 23: 1397-1405, 2000. Go to original source...
    32. Ruiz-Espinoza, F.H., Murillo-Amador, B., García-Hernández, J.L. et al.: Field evaluation of the relationship between chlorophyll content in basil leaves and a portable chlorophyll meter (SPAD-502) readings. - J. Plant Nutr. 33: 423-438, 2010. Go to original source...
    33. Sakshaug, E., Bricaud, A., Dandonneau, Y. et al.: Parameters of photosynthesis: definitions, theory and interpretation of results. - J. Plankton Res. 19: 1637-1670, 1997. Go to original source...
    34. Serôdio, J., Vieira, S., Cruz, S., Barroso, F.: Short-term variability in the photosynthetic activity of microphytobenthos as detected by measuring rapid light curves using variable fluorescence. - Mar. Biol. 146: 903-914, 2005. Go to original source...
    35. Skórska, E.: Comparison of chlorophyll fluorescence parameters of Cucumis Sativus and Mentha Piperita leaves exposed to short-term UV-B irradiation. - Acta Biol. Cracov. Ser. Bot. 53: 16-19, 2011. Go to original source...
    36. Smith, E.L.: Photosynthesis in relation to light and carbon dioxide. - Proc. Natl. Acad. Sci. USA 22: 504-511, 1936. Go to original source...
    37. Teramura, A.H.: Effects of ultraviolet-B radiation on the growth and yield of crop plants. - Physiol. Plant. 58: 415-427, 1983. Go to original source...
    38. Teramura, A.H., Sullivan, J.H.: Effects of UV-B radiation on photosynthesis and growth of terrestrial plants. - Photosynth. Res. 39: 463-473, 1994. Go to original source...
    39. van Kooten, O., Snel, J.F.H.: The use of chlorophyll fluorescence nomenclature in plant stress physiology. - Photosynth. Res. 25: 147-150, 1990. Go to original source...
    40. White, A.J., Critchley, C.: Rapid light curves: A new fluorescence method to assess the state of the photosynthetic apparatus. - Photosynth. Res. 59: 63-72, 1999. Go to original source...
    41. Yang, S.H., Wang, L.J., Li, S.H. et al.: The effects of UV-B radiation on photosynthesis in relation to Photosystem II photochemistry, thermal dissipation and antioxidant defenses in winter wheat (Triticum aestivum L.) seedlings at different growth temperatures. - Funct. Plant Biol. 34: 907-917, 2007. Go to original source...
    42. Yannarelli, G.G., Gallego, S.M., Tomaro, M.L.: Effect of UV-B radiation on the activity and isoforms of enzymes with peroxidase activity in sunflower cotyledons. - Environ. Exp. Bot. 56: 174-181, 2006. Go to original source...
    43. Yao, X.Q., Liu, Q.: Changes in morphological, photosynthetic and physiological responses of Mono Maple seedlings to enhanced UV-B and to nitrogen addition. - Plant Growth Regul. 50: 165-177, 2006. Go to original source...
    44. Yao, Y.N., Xuan, Z.Y., Li, Y., et al.: Effects of ultraviolet-B radiation on crop growth, development, yield and leaf pigment concentration of tartary buckwheat (Fagopyrum tataricum) under field conditions. - Eur. J. Agron. 25: 215-222, 2006. Go to original source...

    Return to the content