Photosynthetica 2013, 51(2):202-214 | DOI: 10.1007/s11099-013-0019-0

Combined effects of light and temperature on growth, photosynthesis, and pigment content in the mat-forming cyanobacterium Geitlerinema amphibium

S. Jod³owska1,*, A. Lata³a1
1 Laboratory of Marine Plant Ecophysiology, Institute of Oceanography, University of Gdañsk, Gdynia, Poland

Geitlerinema amphibium (BA-13), mat-forming cyanobacterium from the southern Baltic Sea, was grown at three irradiances [5, 65, and 125 μmol(photon) m-2 s-1] and three temperatures (15, 22.5, and 30°C). To determine the effect of the investigated factors and their interaction on culture concentration, pigment content, and photosynthetic parameters of cyanobacterium, factorial experiments and two-way analysis of variance (ANOVA) were carried out. Both chlorophyll (Chl) a and phycobilins (PB) were influenced by the irradiance and temperature, but stronger effect was noted in the case of the former one. Chl a and PB concentration per 100 μm of filament dropped above 4-fold with the increasing irradiance. The ratios between individual carotenoids [β-carotene, zeaxanthin, and myxoxanthophyll (Myx)] and Chl a increased significantly with an increase in the irradiance. The greatest fluctuations were observed in the ratio of Myx to Chl a (above 10-fold). Thus, Myx was suggested as the main photoprotective carotenoid in G. amphibium. Based on photosynthetic light response (PI) curves, two mechanisms of photoacclimation in G. amphibium were recognized: a change of photosynthetic units (PSU) number and a change of PSU size. These two mechanisms constituted the base of significant changes in photosynthetic rate and its parameters, such as the compensation point (P C), the initial slope of photosynthetic curve (α), saturation irradiance (E K), maximal photosynthetic rate (P max), and dark respiration rate (R D). The greatest changes were observed in P C values (about 15-fold within the range of the factors tested). Studied parameters showed a wide range of changes, which might indicate G. amphibium ability to acclimatize well to irradiance and temperature, and indirectly might explain the successful growth of cyanobacterium in dynamically changing environmental conditions.

Additional key words: Geitlerinema amphibium; irradiance; microbial mats; photosynthesis; pigments; temperature

Received: September 28, 2011; Accepted: November 29, 2012; Published: June 1, 2013  Show citation

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Jod³owska, S., & Lata³a, A. (2013). Combined effects of light and temperature on growth, photosynthesis, and pigment content in the mat-forming cyanobacterium Geitlerinema amphibium. Photosynthetica, 51(2), 202-214. doi: 10.1007/s11099-013-0019-0
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    References

    1. Allakhverdiev, S.I., Kreslavski, V.D., Klimov, V.V. et al.: Heat stress: an overview of molecular responses in photosynthesis. - Photosynth. Res. 98: 541-550, 2008. Go to original source...
    2. Bidigare, R.R., Schofield, O., Prézelin, B.B.: Influence of zeaxanthin on quantum yield of photosynthesis of Synechococcus clone WH7803 (DC2). - Mar. Ecol. Prog. Ser. 56: 177-188, 1989. Go to original source...
    3. Brzeziñski, J., Stachowski, R.: [Application of variance analysis to experimental psychological investigations]. - PWN, Warszawa 1981. [In Polish.]
    4. Davison, I.R.: Environmental effects on algal photosynthesis: temperature. - J. Phycol. 27: 2-8, 1991. Go to original source...
    5. Defew, E.C., Perkins, R.G., Paterson, D.M.: The influence of light and temperature interactions on a natural estuarine microphytobenthic assemblage. - Biofilms 1: 21-30, 2004. Go to original source...
    6. Dring, M.J.: The Biology of Marine Plants. - Cambridge Univ. Press, Cambridge 1998.
    7. Falkowski, P.G., Dubinsky, Z., Wyman, K.: Growth-irradiance relationships in phytoplankton. - Limnol. Oceanogr. 30: 311-321, 1985. Go to original source...
    8. Fisher, R.A., Yates, F.: Statistical Tables for Biological, Agricultural and Medical Research. - 6th Ed. Olivier and Boyd, Edinburgh 1963.
    9. Fisher, T., Minnaard, J., Dubinsky, Z.: Photoacclimation in the marine alga Nannochloropsis sp. (Eustigmatophyte): a kinetic study. - J. Plankton Res. 18: 1797-1818, 1996. Go to original source...
    10. Fogg, G.E., Thake, B.: Algal Cultures and Phytoplankton Ecology. - Univ. Wisconsin Press, Madison and Milwaukee 1987.
    11. Henley, W.J.: Measurement and interpretation of photosynthetic light-response curves in algae in the context of photoinhibition and diel changes. - J. Phycol. 29: 729-739, 1993. Go to original source...
    12. Hirschberg, J., Chamovitz, D.: Carotenoids in cyanobacteria. - In: Bryant, D.A. (ed.): The Molecular Biology of Cyanobacteria. Pp. 559-579. Kluwer Academic Publishers, Dordrecht - Boston - Lancaster 1994. Go to original source...
    13. Ibelings, B.W.: Changes in photosynthesis in response to combined irradiance and temperature stress in cyanobacterial surface waterblooms. - J. Phycol. 32: 549-557, 1996. Go to original source...
    14. Jensen, S., Knutsen, G.: Influence of light and temperature on photoinhibition of photosynthesis in Spirulina platensis. - J. Appl. Phycol. 5: 495-504, 1993. Go to original source...
    15. Jerlov, N.G.: Marine Optics. - Elsevier, Amsterdam 1976.
    16. Jod³owska, S., Lata³a, A.: Simultaneous separation of chlorophylls and carotenoids by RP-HPLC in some algae and cyanobacteria from the Southern Baltic. - Oceanol. Hydrobiol. Stud. 32: 81-89, 2003.
    17. Kana, T.M., Glibert, P.M., Goericke, R., Welschmeyer, N.A.: Zeaxanthin and β-carotene in Synechococcus WH7803 respond differently to irradiance. - Limnol. Oceanogr. 33: 1623-1627, 1988. Go to original source...
    18. Kana, T.M., Glibert, P.M.: Effect of irradiances up to 2000 μE m-2 s-1 on marine Synechococcus WH7803. II. Photosynthetic responses and mechanisms. - Deep-Sea Res. 34: 497-516, 1987. Go to original source...
    19. Kononen, K.: Dynamics of the toxic cyanobacterial blooms in the Baltic Sea. - Finn. Mar. Res. 261: 3-36, 1992.
    20. Lakatos, M., Bilger, W., Büdel, B.: Carotenoid composition of terrestrial Cyanobacteria: response to natural light conditions in open rock habitats in Venezuela. - Eur. J. Phycol. 36: 367-375, 2001. Go to original source...
    21. Lata³a, A., Jod³owska, S., Pniewski, F.: Culture Collection of Baltic Algae (CCBA) and characteristic of some strains by factorial experiment approach. - Arch. Hydrobiol. 165, Algological Studies 122: 137-154, 2006. Go to original source...
    22. Lata³a, A., Misiewicz, S.: Effects of light, temperature and salinity on the growth and chlorophyll-a content of Baltic cyanobacterium Phormidium sp. - Arch. Hydrobiol. 136, Algological Studies 100: 157-180, 2000. Go to original source...
    23. Lata³a, A.: Photosynthesis and respiration of plants from the Gulf of Gdañsk. - Acta Ichthyol. Piscat. 21: 85-100, 1991. Go to original source...
    24. MacIntyre, H.L., Kana, T.M., Anning, T., Geider, R.J.: Photoacclimation of photosynthesis irradiance response curves and photosynthetic pigments in microalgae and cyanobacteria. - J. Phycol. 38: 17-38, 2002. Go to original source...
    25. Millie, D.F., Hersh, C.M., Dionigi, C.P.: Simazine-induced inhibition in photoacclimated populations of Anabaena circinalis (Cyanophyta). - J. Phycol. 28: 19-26, 1992. Go to original source...
    26. Millie, D.F., Ingram, D.A., Dionigi, C.P.: Pigment and photosynthetic responses of Oscillatoria agardhii (Cyanophyta) to photon flux density and spectral quality. - J. Phycol. 26: 660-666, 1990. Go to original source...
    27. Mouget, J.-L., Tremblin, G., Morant-Manceau, A. et al.: Longterm photoacclimation of Haslea ostrearia (Bacillariophyta): effect of irradiance on growth rates, pigment content and photosynthesis. - Eur. J. Phycol. 34: 109-115, 1999. Go to original source...
    28. Oktaba, W.: [Methods of mathematical statistic in experiments]. - PWN, Warszawa 1986. [In Polish.]
    29. Pearl, H.W., Tucker, J., Bland, P.T.: Carotenoid enhancement and its role in maintaining blue-green algal (Microcystis aeruginosa) surface blooms. - Limnol. Oceanogr. 28: 847-857, 1983. Go to original source...
    30. Platt, T., Jassby, A.D.: The relationship between photosynthesis and light for natural assemblages of coastal marine phytoplankton. - J. Phycol. 12: 421-430, 1976. Go to original source...
    31. Platt. T, Gallegos, C.L., Harrison, W.G.: Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton. - J. Mar. Res. 38: 687-701, 1980.
    32. Prézelin, B.B.: Light reactions in photosynthesis. - In: Platt, T. (ed.): Physiological Bases of Phytoplankton Ecology. Pp. 1-43. Can. Bull. Fish. Aquat. Sci., no. 210, Ottawa 1981.
    33. Prézelin, B.B and Matlick, H.A.: Time-course of photoadaptation in the photosynthesis-irradiance relationship of a dinoflagellate exhibiting photosynthesis periodicity. - Mar. Biol. 5: 85-96, 1980. Go to original source...
    34. Rabinowitch, E.I.: Photosynthesis and Related Processes. Vol. II, part 1. - Interscience Publishers, New York 1951. Go to original source...
    35. Ramus, J.: The capture and transduction of light energy. - In: Lobban, C.S., Wynne, M.J. (ed.): The Biology of Seaweeds. Pp. 458-492. Blackwell Scientific, Oxford 1981.
    36. Raps, S., Wyman, K., Siegelman, H.W., Falkowski, P.G.: Adaptation of the cyanobacterium Microcystis aeruginosa to light intensity. - Plant Physiol. 72: 829-832, 1983. Go to original source...
    37. Rau, W.: Functions of carotenoids other than in photosynthesis. - In: Goodwin, T.W. (ed.): Plant Pigments. Pp. 231-255. Academic Press, London 1988.
    38. Richardson, K., Beardall, J., Raven, J.A.: Adaptation of unicellular algae to irradiance: an analysis of strategies. - New Phytol. 93: 157-191, 1983. Go to original source...
    39. Roos, J.C., Vincent, W.F.: Temperature dependence of UV radiation effects on Antarctic cyanobacteria. - J. Phycol. 34: 118-125, 1998. Go to original source...
    40. Skulberg, O.M.: Oscillatorialean cyanoprokaryotes and their application for algal culture technology. - Arch. Hydrobiol. 105, Algol. Stud. 75: 265-278, 1994. Go to original source...
    41. Snedecor, G.W., Cochran, W.G.: Statistical Methods. - Iowa State University Press, Ames 1980.
    42. Stanier, R.Y., Kunisawa, R., Mandel, M., Cohen-Bazire, G.: Purification and properties of unicellular blue-green algae (order Chroococcales). - Bacteriol. Rev. 35: 171-205, 1971. Go to original source...
    43. Steiger, S., Schäfer, L., Sandmann, G.: High-light-dependent upregulation of carotenoids and their antioxidative properties in the cyanobacterium Synechocystis PCC 6803. - J. Photoch. Photobiol. B. 52: 14-18, 1999. Go to original source...
    44. Stewart, D.E., Farmer, F.H.: Extraction, identification, and quantitation of phycobiliprotein pigments from phototrophic plankton. - Limnol. Oceanogr. 29: 392-397, 1984. Go to original source...
    45. Takahashi, S., Badger, M.R.: Photoprotection in plants: A new light on photosystem II damage. - Trends Plant Sci. 16: 53-60, 2011. Go to original source...
    46. Takahashi, S., Murata, N.: How do environmental stresses accelerate photoinhibition? - Trends Plant Sci. 13: 178-182, 2008. Go to original source...
    47. Tandeau de Marsac, N.: Chromatic adaptation by cyanobacteria. - In: Bogorad, L., Vasil, I.K. (ed.): Cell Culture and Somatic Cell Genetics of Plants. Pp. 417-446. Academic Press, San Diego - New York - Boston - London - Syney - Tokyo - Toronto 1991. Go to original source...
    48. Tandeau de Marsac, N., Houmard, J.: Complementary chromatic adaptation: physiological conditions and action spectra. - In: Packer, L., Glazer, A.N. (ed.): Methods in Enzymology. Pp. 318-328. Academic Press, New York 1988. Go to original source...
    49. Tang, E.P.Y., Tremblay, R., Vincent, W.F.: Cyanobacterial dominance of polar freshwater ecosystems: are high-latitude mat-formers adapted to low temperature? - J. Phycol. 33: 171-181, 1997. Go to original source...
    50. Wilhelm, C.: The biochemistry and physiology of light harvesting processes in chlorophyll-band c-containing algae. - Plant Physiol. Biochem. 28: 293-306, 1993.
    51. Witkowski, A.: Microbial mat with an incomplete vertical structure, from brackish-water environment, the Puck Bay, Poland, a possible analog of an "advanced anaerobic ecosystem"? - Orig. Life Evol. Biosph. 16: 337-338, 1986. Go to original source...
    52. Zurzycki, J., Starzecki, W.: Volumetric methods. - In: ©esták, Z., Èatský, J., Jarvis, P.G. (ed.): Plant Photosynthetic Production Manual of Methods. Pp. 257-275. Dr. W. Junk N.V. Publishers, The Hague 1971.

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