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Tools for biotechnological production of useful phytochemicals from adventitious root cultures

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Abstract

Adventitious roots are natural roots which develop from the differentiated cells of any organ such as leaf, root or stem. These roots are attractive source of phytochemicals due to their genetic and biosynthetic stability. Their stability offers new scope for the in vitro production of valuable secondary metabolites. In the past two decades, adventitious roots have been induced from different plant systems and the suspension cultures have been established. However, the special morphology of adventitious roots hampers the scale-up processes, which needs modifications with the existing bioreactors to facilitate proper mixing, aeration and which can overcome shear stress. Various modifications have been done with the stirred tank and air-lift bioreactors and successfully used for the cultivation of adventitious roots. The progress made in this area is tremendous, in few cases adventitious root cultures are succeeded up to commercial scale with step-wise viz. small scale to large-scale by establishing the standards with various parameters which affect the biomass accumulation and metabolite productivity. This review addresses the recent progresses made in this area and discusses the potential applications and limitations.

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Acknowledgments

This study was supported by a grant from the Korean Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (Grant No. A103017). HNM is thankful to the Ministry of Education, Science, and Technology, Republic of Korea for the Brain Pool Fellowship (131S-4-3-0523). The Ministry of Science, ICT, and Planning (MSIP) also supported this study.

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Authors and Affiliations

  1. Research Center for the Development Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Hosakatte Niranjana Murthy & Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, 580003, India

    Hosakatte Niranjana Murthy & Vijayalaxmi S. Dandin

Authors
  1. Hosakatte Niranjana Murthy
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  2. Vijayalaxmi S. Dandin
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  3. Kee-Yoeup Paek
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Correspondence to Hosakatte Niranjana Murthy or Kee-Yoeup Paek.

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Murthy, H.N., Dandin, V.S. & Paek, KY. Tools for biotechnological production of useful phytochemicals from adventitious root cultures. Phytochem Rev 15, 129–145 (2016). https://doi.org/10.1007/s11101-014-9391-z

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  • DOI: https://doi.org/10.1007/s11101-014-9391-z

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