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Arctic and subarctic soil populations of Rhizobium leguminosarum biovar trifolii nodulating three different clover species: Characterisation by diversity at chromosomal and symbiosis loci

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Abstract

Indigenous soil populations of Rhizobium leguminosarum biovar trifolii from Arctic and subarctic regions have been characterised with emphasis on chromosomal and symbiotic genes. Three clover species were used to trap rhizobia from soils along a latitudinal gradient from 78°N to 60°N in Norway. For the first time R. l. bv. trifolii was isolated from Svalbard at 78°N. Under the extreme conditions in the Arctic, rhizobia have survived as saprophytes and in symbiosis with clover legumes. The chromosomal diversity of the soil populations was mapped by rep-PCR. Separation of chromosomal types were strongly influenced by geographic origin. Symbiotic genes, the nodEF and nifDK IGS gene regions, were investigated by PCR-RFLP. The nifDK IGS were more conserved than the nodEF genes. Sym plasmids were widely distributed in different chromosomal types and across the latitudinal gradient.

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Abbreviations

ERIC:

Enterobacterial repetitive intergenic consensus

RFLP:

Restriction fragment length polymorphism

IGS:

Intergene sequences

References

  • F J Bergersen (1961) ArticleTitleThe growth of Rhizobium in synthetic media Aust. J. Biol. Sci. 14 349–360 Occurrence Handle1:CAS:528:DyaF38XitVCnsg%3D%3D

    CAS  Google Scholar 

  • Bruijn Particlede (1992) ArticleTitleUse of repetitive (repetitive extragenic palindromic and enterobacterial repetitive intergeneric consensus) sequences and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria Appl. Environ. Microbiol. 58 2180–2187 Occurrence Handle1637156

    PubMed  Google Scholar 

  • G E Carlson P B Gibson D D Baltensperger (1985) White clover and other perennial clovers ME Heath RF Barnes DS Metcalfe (Eds) Forages: The Science of Grassland Agriculture Iowa State Univ. Presss Ames 118–127

    Google Scholar 

  • W -M Chen L Moulin C Bontemps P Vandamme G Béna C Boivin-Masson (2003) ArticleTitleLegume symbiotic nitrogen fixation is widespread in nature J. Bacteriol. 185 7266–7272 Occurrence Handle14645288 Occurrence Handle1:CAS:528:DC%2BD3sXpvVaqtro%3D

    PubMed  CAS  Google Scholar 

  • P Drouin D Prevost H Antoun (1996) ArticleTitleClassification of bacteria nodulating Lathyrus japonicus and Lathyrus pratensis in northern Quebec as strains of Rhizobium leguminosarum biovar viciae Int. J. Syst. Bacteriol. 46 IssueID4 1016–1024 Occurrence Handle8863431 Occurrence Handle1:STN:280:DyaK2s%2Fhs1yjtw%3D%3D Occurrence Handle10.1099/00207713-46-4-1016

    Article  PubMed  CAS  Google Scholar 

  • P Drouin D Prévost H Antoun (2000) ArticleTitlePhysiological adaptation to low temperatures of strains of Rhizobium leguminosarum bv. Viciae associated with Lathyrus spp FEMS. Microbiol. Ecol. 32 111–120 Occurrence Handle10817864 Occurrence Handle1:CAS:528:DC%2BD3cXjt1ejtrk%3D

    PubMed  CAS  Google Scholar 

  • A Economou A E Davies A W B Johnston J A Downie (1994) ArticleTitleThe Rhizobium leguminosarum biovar viciae nodO gene can enable a nodE mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch Microbiology 140 2341–2347 Occurrence Handle1:CAS:528:DyaK2cXmt1Kit7w%3D Occurrence HandleA1994PG51800022 Occurrence Handle10.1099/13500872-140-9-2341

    Article  CAS  ISI  Google Scholar 

  • P H Graham (1992) ArticleTitleStress tolerance in Rhizobium and Bradyrhizobium, and nodulation under adverse soil conditions Can. J. Microbiol. 38 475–484 Occurrence Handle1:CAS:528:DyaK38Xlt1Ort7Y%3D Occurrence Handle10.1139/m92-079

    Article  CAS  Google Scholar 

  • N J Hahn (1966) ArticleTitleThe congo red reaction in bacteria and its usefulness in the identification of rhizobia Can. J. Microbiol. 12 725–733 Occurrence Handle5969335 Occurrence Handle1:STN:280:DyaF2s7nsVWmtA%3D%3D Occurrence Handle10.1139/m66-099

    Article  PubMed  CAS  Google Scholar 

  • B A Handley A J Hedges J E Beringer (1998) ArticleTitleImportance of host plants for detecting the population diversity of Rhizobium leguminosarum biovar viciae in soil Soil Biol. Biochem. 30 241–249 Occurrence Handle1:CAS:528:DyaK1cXnslGisw%3D%3D Occurrence Handle10.1016/S0038-0717(97)00103-X

    Article  CAS  Google Scholar 

  • S P Harrison D G Jones J P W Young (1989) ArticleTitleRhizobium population genetics: Genetic variation within and between populations from diverse locations J. Gen. Microbiol. 135 1061–1069 Occurrence Handle1:CAS:528:DyaL1MXkt1Sksbw%3D

    CAS  Google Scholar 

  • K Huss-Danell H Sverisson A -S Hahlin K Danell (1999) ArticleTitleOccurrence of Alnus-infective Frankia and Trifolium-infective Rhizobium in circumpolar soils Arc. Antarc. Alp. Res. 31 400–406

    Google Scholar 

  • M F Hynes M P O`Conell (1990) ArticleTitleHost plant effect on competition among strains of Rhizobium leguminosarum Can. J. Microbiol. 36 864–869 Occurrence Handle10.1139/m90-150

    Article  Google Scholar 

  • S Jamann M P Fernandez P Normand (1993) ArticleTitleTyping method for N2-fixing bacteria based on PCR-RFLP-application to the characterization of Frankia strains Mol. Ecol. 2 17–26 Occurrence Handle7910092 Occurrence Handle1:CAS:528:DyaK3sXkt1Wnu7k%3D

    PubMed  CAS  Google Scholar 

  • D C Jordan (1984) Family III. Rhizobiaceae Conn 1938 NR Krieg JG Holt (Eds) Bergey´s Manual of Systematic Bacteriology NumberInSeriesVol. 1. The Williams & Wilkins Co. Baltimore 234–244

    Google Scholar 

  • G Laguerre P Mavingui M -R Allard M-P Charnay P Louvrier S -I Mazurier L Rigottier-Gois N Amarger (1996) ArticleTitleTyping of Rhizobia by PCR DNA fingerprinting and PCR-Restriction fragment analysis of chromosomal and symbiotic gene regions: Application to Rhizobium leguminosarum and its different biovars Appl. Environ. Microbiol. 62 2029–2036 Occurrence Handle8787401 Occurrence Handle1:CAS:528:DyaK28XjtlGgs7g%3D

    PubMed  CAS  Google Scholar 

  • G Laguerre P Louvrier M -R Allard N Amarger (2003) ArticleTitleCompatibility of rhizobial genotypes within natural populations of Rhizobium leguminosarum biovar viciae for nodulation of host legumes Appl. Environ. Microbiol. 69 2276–2283 Occurrence Handle12676710 Occurrence Handle1:CAS:528:DC%2BD3sXivFKqsb4%3D Occurrence Handle10.1128/AEM.69.4.2276-2283.2003

    Article  PubMed  CAS  Google Scholar 

  • S M Lohrke J H Orf E Martínez-Romero M J Sadowsky (1995) ArticleTitleHost-controlled restriction of nodulation by Bradyrhizobium japonicum strains in serogroup 110 Appl. Environ. Microbiol. 61 2378–2383 Occurrence Handle1:CAS:528:DyaK2MXlvFOgurw%3D Occurrence Handle16535054

    CAS  PubMed  Google Scholar 

  • N Z Lupwayi P M Stephens M J Noonan (1996) ArticleTitleRelationship between timing of infection and nodulation competitiveness of Rhizobium meliloti Symbiosis 21 233–248 Occurrence HandleA1996WG45000004

    ISI  Google Scholar 

  • L Moulin A Munvie B Dreyfus C Boivin-Masson (2001) ArticleTitleNodulation of legumes by members of the β-subclass of Proteobacteria Nature 411 948–950 Occurrence Handle11418858 Occurrence Handle1:CAS:528:DC%2BD3MXkvVWhu7Y%3D Occurrence Handle000169386200044 Occurrence Handle10.1038/35082070

    Article  PubMed  CAS  ISI  Google Scholar 

  • K M Palmer J P W Young (2000) ArticleTitleHigher diversity of Rhizobium leguminosarum biovar viciae population in arable soils than in grass soils Appl. Environ. Microbiol. 66 2445–2450 Occurrence Handle10831423 Occurrence Handle1:CAS:528:DC%2BD3cXjvFWnsrg%3D

    PubMed  CAS  Google Scholar 

  • S Rome B Brunel P Normand M Fernandez J -C Cleyet-Marel (1996) ArticleTitleEvidence that two genomic species of Rhizobium are associated with Medicago truncatula Arch. Microbiol. 165 285–288 Occurrence Handle8639029 Occurrence Handle1:STN:280:DyaK283hsVKmuw%3D%3D Occurrence Handle10.1007/s002030050328

    Article  PubMed  CAS  Google Scholar 

  • R J Roughley (1984) Plant nutrient solutions P Somasegaran H J Hoben (Eds) Handbook for Rhizobia: Methods in Legume-Rhizobium Technology Springer-Verlag New York 339–341

    Google Scholar 

  • Rønningen O I 1979 Svalbards flora. Polarhåndbok Nr 1. Norsk Polarinstitutt, Oslo

  • G B Ruvkun F M Ausubel (1980) ArticleTitleInterspecies homology of nitrogenase genes Proc. Natl. Acad. Sci. USA 77 191–195 Occurrence Handle6987649 Occurrence Handle1:CAS:528:DyaL3cXht1Cks74%3D

    PubMed  CAS  Google Scholar 

  • P Somasegaran H J Hoben (1994a) Handbook for Rhizobia: Methods in Legume-Rhizobium Technology Springer-Verlag New York, Inc 366–369

    Google Scholar 

  • P Somasegaran H J Hoben (1994b) Handbook for Rhizobia: Methods in Legume-Rhizobium Technology Springer-Verlag New York, Inc 336–337

    Google Scholar 

  • H P Spaink J Weinman M A Djordjevic C A Wijffelman R J H Okker B J J Lugtenberg (1989) ArticleTitleGenetic analysis and cellular localization of the Rhizobium host specificity-determining NodE protein EMBO J. 8 2811–2818 Occurrence Handle2684629 Occurrence Handle1:CAS:528:DyaK3cXpt1Kqtg%3D%3D

    PubMed  CAS  Google Scholar 

  • Svenning M M 1991 Adaptation of white clover and Rhizobium to subarctic temperature and light conditions. Doctor Scientarium Theses, University of Tromsø

  • M M Svenning K Røsnes L Lund O Junttila (2001) ArticleTitleVegetative growth and freezing tolerance of white clover (Trifolium repens L.) genotypes from Svalbard Acta. Agric. Scand. 51 10–16

    Google Scholar 

  • E Velázquez P F Mateos N Velasco et al. (1999) ArticleTitleSymbiotic characteristic and selection of autochtomous strains of Sinorhizobium meliloti populations in different soils Soil Biol. Biochem. 31 1039–1047

    Google Scholar 

  • J Versalovic T Koeuth J R Lupski (1991) ArticleTitleDistribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes Nucleic. Acids. Res. 19 6923–6831

    Google Scholar 

  • Wilson K 1994 Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology. Eds. Ausubel FM Brent R Kingstone RE. et al. pp. 2.4.1–2.4.5. John Wiley & Sons, Inc.

  • J P W Young (1996) ArticleTitlePhylogeny and taxonomy of rhizobia Plant Soil. 186 45–52 Occurrence Handle1:CAS:528:DyaK2sXmvVWnuw%3D%3D Occurrence Handle10.1007/BF00035054

    Article  CAS  Google Scholar 

  • A Zèzè L A Mutch J P W Young (2001) ArticleTitleDirect amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil Environ. Microbiol. 3 363–370 Occurrence Handle11472501

    PubMed  Google Scholar 

  • X X Zhang B Kosier U B Priefer (2001) ArticleTitleGenetic diversity of indigenous Rhizobium leguminosarum bv. Viciae isolates nodulating two different host plants during soil restoration with alfalfa Mol. Ecol. 10 2297–2305 Occurrence Handle11555271 Occurrence Handle1:STN:280:DC%2BD3MrgslWhsg%3D%3D Occurrence Handle10.1046/j.0962-1083.2001.01364.x

    Article  PubMed  CAS  Google Scholar 

  • L Øvreås V Torsvik (1998) ArticleTitleMicrobial diversity and community structure in two different agricultural soil communities Microbiol. Ecol. 36 303–315

    Google Scholar 

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  1. Department of Biology, Faculty of Science, University of Tromsö, 9037, Tromsö, Norway

    Inger Lise Fagerli & Mette M. Svenning

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  1. Inger Lise Fagerli
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  2. Mette M. Svenning
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Correspondence to Mette M. Svenning.

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Fagerli, I.L., Svenning, M.M. Arctic and subarctic soil populations of Rhizobium leguminosarum biovar trifolii nodulating three different clover species: Characterisation by diversity at chromosomal and symbiosis loci. Plant Soil 275, 371–381 (2005). https://doi.org/10.1007/s11104-005-3103-9

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