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5-Aminolevulinic acid improves photosynthetic gas exchange capacity and ion uptake under salinity stress in oilseed rape (Brassica napus L.)

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Abstract

Salinity is one of the major constraints in oilseed rape (Brassica napus L.) production. One of the means to overcome this constraint is the use of plant growth regulators to induce plant tolerance. To study the plant response to salinity in combination with a growth regulator, 5-aminolevulinic acid (ALA), oilseed rape plants were grown hydroponically in greenhouse conditions under three levels of salinity (0, 100, and 200 mM NaCl) and foliar application of ALA (30 mg/l). Salinity depressed the growth of shoots and roots, and decreased leaf water potential and chlorophyll concentration. Addition of ALA partially improved the growth of shoots and roots, and increased the leaf chlorophyll concentrations of stressed plants. Foliar application of ALA also maintained leaf water potential of plants growing in 100 mM salinity at the same level as that of the control plants, and there was also an improvement in the water relations of ALA-treated plants growing in 200 mM. Net photosynthetic rate and gas exchange parameters were also reduced significantly with increasing salinity; these effects were partially reversed upon foliar application with ALA. Sodium accumulation increased with increasing NaCl concentration which induced a complex response in the macro-and micronutrients uptake and accumulation in both roots and leaves. Generally, analyses of macro- (N, P, K, S, Ca, and Mg) and micronutrients (Mn, Zn, Fe, and Cu) showed no increased accumulation of these ions in the leaves and roots (on dry weight basis) under increasing salinity except for zinc (Zn). Foliar application of ALA enhanced the concentrations of all nutrients other than Mn and Cu. These results suggest that under short-term salinity-induced stress (10 days), exogenous application of ALA helped the plants improve growth, photosynthetic gas exchange capacity, water potential, chlorophyll content, and mineral nutrition by manipulating the uptake of Na+.

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Acknowledgements

This work was supported by the National Key Science & Technology Supporting Program of China (2010BAD01B04), the National High Technology Research & Development Program of China (2006AA10A214), the Industry Technology System of Rapeseed in China (nycytx-005), Zhejiang Provincial Natural Science Foundation (R307095), the Science & Technology Department of Zhejiang Province (2008C22078), and the Higher Education Commission of Pakistan.

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Authors and Affiliations

  1. Institute of Crop Science, Zhejiang University, Hangzhou, 310029, China

    M. S. Naeem, Z. L. Jin, G. L. Wan, D. Liu, H. B. Liu & W. J. Zhou

  2. Weed Science Center, Utsunomiya University, Utsunomiya, 321-8505, Japan

    K. Yoneyama

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  1. M. S. Naeem
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  2. Z. L. Jin
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  3. G. L. Wan
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  4. D. Liu
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  5. H. B. Liu
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  6. K. Yoneyama
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  7. W. J. Zhou
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Correspondence to W. J. Zhou.

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Responsible Editor: Timothy J. Flowers.

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Naeem, M.S., Jin, Z.L., Wan, G.L. et al. 5-Aminolevulinic acid improves photosynthetic gas exchange capacity and ion uptake under salinity stress in oilseed rape (Brassica napus L.). Plant Soil 332, 405–415 (2010). https://doi.org/10.1007/s11104-010-0306-5

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