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The functional sites of chlorophylls in D1 and D2 subunits of Photosystem II identified by pulsed EPR

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Abstract

The functional site of ChlZ, an auxiliary electron donor to P680+, was determined by pulsed ELDOR applied to a radical pair of Y •D and Chlz+ in oriented PS II membranes from spinach. The radical-radical distance was determined to be 29.5 Å and its direction was 50° from the membrane normal, indicating that a chlorophyll on the D2 protein is responsible for the EPR Chlz+ signal. Spin polarized ESEEM (Electronin Spin Echo Envelop Modulation) of a 3Chl and Q −A radical pair induced by a laser flash was observed in reaction center D1D2Cytb559 complex, in which QA was functionally reconstituted with DBMIB and reduced chemically. Q −A ESEEM showed a characteristic oscillating time profile due to dipolar coupling with 3Chl. By fitting with the dipolar interaction parameters, the distance between 3Chl and Q −A was determined to be 25.9 Å, indicating that the accessory chlorophyll on the D1 protein is responsible for the 3Chl signal.

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Abbreviations

PS II :

Photosystem II

Chl:

chlorophyll

RC:

reaction center

DBMIB:

2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone

DTN:

sodium dithionite

YD:

tyrosine D

YZ:

tyrosine Z

QA and QB:

primary and secondary electron acceptor quinones

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Authors and Affiliations

  1. School of Science and Technology, Kwansei Gakuin University, Sanda, Japan

    A. Kawamori

  2. The Institute of Physical and Chemical Research (RIKEN), Photodynamics Research Center, Sendai, Japan

    T-A. Ono, A. Ishii & S. Nakazawa

  3. Division of EPR Application, Bruker Biospin, KK., Tsukuba, Japan

    H. Hara

  4. Department of Physics, College of Humanities and Sciences, Nihon University, Tokyo, Japan

    T. Tomo

  5. Low Temperature Research Institute, Hokkaido University, Sapporo, Japan

    J. Minagawa

  6. Institute of Experimental Physics, Free University of Berlin, Berlin, Germany

    R. Bittl

  7. Institute of Chemical Kinetics and Combustion, Russian Academy of Sciences, Novosibirsk, Russia

    S.A. Dzuba

Authors
  1. A. Kawamori
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  2. T-A. Ono
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  3. A. Ishii
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  4. S. Nakazawa
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  5. H. Hara
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  6. T. Tomo
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  7. J. Minagawa
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  8. R. Bittl
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  9. S.A. Dzuba
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Correspondence to A. Kawamori.

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Kawamori, A., Ono, TA., Ishii, A. et al. The functional sites of chlorophylls in D1 and D2 subunits of Photosystem II identified by pulsed EPR. Photosynth Res 84, 187–192 (2005). https://doi.org/10.1007/s11120-005-1000-y

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  • DOI: https://doi.org/10.1007/s11120-005-1000-y

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