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Resonance Raman spectroscopy

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Abstract

Resonance Raman spectroscopy may yield precise information on the conformation of, and on the interactions assumed by, the chromophores involved in the first steps of the photosynthetic process, whether isolated in solvents, embedded in soluble or membrane proteins, or, as shown recently, in vivo. By making use of this technique, it is possible, for instance, to relate the electronic properties of these molecules to their structure and/or the physical properties of their environment, or to determine subtle changes of their conformation associated with regulatory processes. After a short introduction to the physical principles that govern resonance Raman spectroscopy, the information content of resonance Raman spectra of chlorophyll and carotenoid molecules is described in this review, together with the experiments which helped in determining which structural parameter each Raman band is sensitive to. A selection of applications of this technique is then presented, in order to give a fair and precise idea of which type of information can be obtained from its use in the field of photosynthesis.

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Notes

  1. Stokes and anti-Stokes Raman shifts are named after George Gabriel Stokes (1819–1903).

Abbreviations

BChl:

Bacteriochlorophyll

Chl:

Chlorophyll

LH:

Light-harvesting

LHCII:

Major light-harvesting protein from higher plants

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Acknowledgments

The author would like to acknowledge generous support from the French National Research Agency (ANR, programs MASTRIT and CAROPROTECT). This manuscript was edited by Govindjee.

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Authors and Affiliations

  1. Institute of Biology and Technology of Saclay, CEA (Commissariat à l’Energie Atomique), URA 2096 CNRS (Centre National de la Recherche Scientifique), 91191, Gif sur Yvette, France

    Bruno Robert

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Correspondence to Bruno Robert.

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Robert, B. Resonance Raman spectroscopy. Photosynth Res 101, 147–155 (2009). https://doi.org/10.1007/s11120-009-9440-4

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