Speckle dynamics in a thin biological object illuminated through a diffusor box is studied both theoretically and experimentally. A model that allows for random time variations in the phase difference Δφ of pairs of waves transmitted through the object is used in the theoretical part of this study. Formulas for the time-averaged intensity of the radiation at a certain point of observation and a temporal autocorrelation function of the radiation intensity are deduced. The relationship between the characteristics of the random variable Δφ and the parameters of the speckle dynamics is discussed. The case of occurrence of a quasiperiodic variation in the radiation intensity is separately considered. The theoretical results are used for interpretation of the data obtained in the experiments on studying the metabolic activity of cells cultivated on a glass substrate.
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Translated from Izvestiya Vysshikh Uchebnykh Zavedenii, Radiofizika, Vol. 57, No. 8, pp. 632–645, August–September 2014.
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Vladimirov, A.P. Dynamic Speckle-Interferometry of Microscopic Processes in Thin Biological Objects. Radiophys Quantum El 57, 564–576 (2015). https://doi.org/10.1007/s11141-015-9540-2
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DOI: https://doi.org/10.1007/s11141-015-9540-2