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Photoperiodic and Hormonal Control of Tuberization in Potato Plants Transformed with the PHYB Gene from Arabidopsis

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Abstract

We studied photoperiodic and hormonal regulation of tuberization in wild-type potato (Solanum tuberosum L., cv, Desiree) plants and derivative transgenic plants harboring the PHYB gene from Arabidopsis, which encodes the phytochrome B apoprotein, under the control of the cauliflower mosaic virus 35S promoter. Plants were cultured on hormone-free Murashige and Skoog nutrient medium containing 5% sucrose or on the same medium supplemented with 1 mg/l kinetin under conditions of long day (LD, 16 h), short day (SD, 10 h), or SD with interrupted long night. We estimated cytokinins (zeatin and zeatin riboside) in underground and aboveground plant organs by the ELISA technique and GA activity in a bioassay with dwarf pea seedlings. Under LD conditions, transgenic plants produced substantially less tubers than wild-type plants. Kinetin addition to the culturing medium resulted in stimulation of tuberization under LD conditions, especially pronounced in the PHYB plants. The content of cytokinins and the activity of GA were much higher under LD conditions, especially in leaves. The total level of both phytohormones was higher in transformed as compared to wild-type plants. A relation of phytochrome-dependent tuberization to the hormonal status of underground and above-ground plant organs and possible reasons for kinetin stimulatory effect on this process are discussed.

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Abbreviations

Cwt :

control wild-type plants

DI:

darkness interruption

LD:

long day

MS:

Murashige and Skoog nutrient medium

SD:

short day

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    N. P. Aksenova, T. N. Konstantinova, V. N. Lozhnikova, S. A. Golyanovskaya, I. A. Gukasyan & G. A. Romanov

  2. Institute of Plant Science, Goettingen University, D-37073, Goettingen, Germany

    C. Gatz

Authors
  1. N. P. Aksenova
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  2. T. N. Konstantinova
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  3. V. N. Lozhnikova
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  4. S. A. Golyanovskaya
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  5. I. A. Gukasyan
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  6. C. Gatz
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  7. G. A. Romanov
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__________

Translated from Fiziologiya Rastenii, Vol. 52, No. 5, 2005, pp. 701–707.

Original Russian Text Copyright © 2005 by Aksenova, Konstantinova, Lozhnikova, Golyanovskaya, Gukasyan, Gatz, Romanov.

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Aksenova, N.P., Konstantinova, T.N., Lozhnikova, V.N. et al. Photoperiodic and Hormonal Control of Tuberization in Potato Plants Transformed with the PHYB Gene from Arabidopsis . Russ J Plant Physiol 52, 623–628 (2005). https://doi.org/10.1007/s11183-005-0092-8

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  • DOI: https://doi.org/10.1007/s11183-005-0092-8

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