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Selection of atrazine-resistant plants by in vitro mutagenesis in pepper (Capsicum annuum)

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Abstract

The effects of atrazine on cotyledon cultures of Capsicum annuum (L.) cv. G4 were investigated with a view of establishing a system for in vitro selection of resistant mutants. At low levels of herbicide produced little growth inhibition, some chlorophyll loss occurred associated with the production of albino shoots. At 20 mg l−1 atrazine bleaching was more pronounced and was accompanied by the development of necrotic spots; however, efficient bleaching was associated with severe suppression of growth. Mutagenized cotyledon explants resulted in production of herbicide-resistant plants on medium containing selective levels of sucrose (0.5%) and atrazine (20 mg l−1). Differential morphogenetic responses were observed when the levels of sucrose (0.5–5%) were altered. Shoot regeneration was maximum in 2 sucrose and the regenerating ability decreased with a further increase in sucrose concentration (3%–5%). However, lowering of sucrose concentration from 2 to 0.5% caused complete bleaching of explants and permitted the selection of herbicide-resistant plants. Complete atrazine-resistant plantlets were obtained after rooting of regenerated green shoots on rooting medium containing 10 mg l−1atrazine, 1.0 mg l−1IAA and 0.5% sucrose. Leaf-segment assay of differentiated plants revealed that all regenerants were resistant to the atrazine. Reciprocal crosses between atrazine-resistant and -sensitive plants showed a non-Mendelian transmission of resistance trait.

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Abbreviations

2,4-D:

2,4-dichlorophenoxy acetic acid

BA:

benzyladenine

EMS:

ethyl methane sulphonate

IAA:

3-indole acetic acid

Kin:

kinetin

MS:

Murashige and Skoog

NEU:

nitroso ethyl urea

NMU:

nitroso methyl urea

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Kakatiya University, Warangal, 506 009, A.P, India

    Peddaboina Venkataiah, Thamidala Christopher & Subhash Karampuri

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  1. Peddaboina Venkataiah
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  2. Thamidala Christopher
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  3. Subhash Karampuri
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Correspondence to Peddaboina Venkataiah.

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Venkataiah, P., Christopher, T. & Karampuri, S. Selection of atrazine-resistant plants by in vitro mutagenesis in pepper (Capsicum annuum). Plant Cell Tiss Organ Cult 83, 75–82 (2005). https://doi.org/10.1007/s11240-005-2678-4

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  • DOI: https://doi.org/10.1007/s11240-005-2678-4

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