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Effect of Light on Gene Expression and Shikonin Formation in Cultured Onosma Paniculatum Cells

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Abstract

The cell culture in a production medium in the dark is capable of producing a large quantity of shikonin and its derivatives, which were completely inhibited when the cell cultures were irradiated with continuous white light and blue light and partially repressed under continuous red light. The expressions of PAL1, 4CL1, and CYP98A6 were induced under continuous red, blue and white light. Transcript levels of HMGR and LDI2 gradually were decreased in the dark and under continuous red light, while no transcriptional product of LDI2 was detected under blue and white light. C4H2 and LePGT1 genes were constantly expressed irrespective of different light conditions. Among the genes studied, the expression of LDI2 was critical for light-regulated shikonin formation. The inducible expression of PAL1, 4CL1, and CYP98A6 as well as the inhibitory transcription of LDI2 mediated by continuous red light irradiation were likely accounted for the reduced accumulation of shikonin.

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Authors and Affiliations

  1. State Key Laboratory of Pharmaceutical Biotechnology, Plant Cell and Molecular Biology Laboratory, Department of Biological Science and Technology, School of Life Sciences, Nanjing University, 210093, Nanjing, P.R. China

    Zhi Liu, Jin-Liang Qi, Lu Chen, Ming-Sheng Zhang, Xiu-Qiang Wang, Yan-Jun Pang & Yong-Hua Yang

  2. Department of Biotechnology, Hunan Agricultural University, 410128, Changsa, P.R. China

    Zhi Liu

  3. School of Agronomy, Guizhou University, 550025, Guiyang, P.R. China

    Ming-Sheng Zhang

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  1. Zhi Liu
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  2. Jin-Liang Qi
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  4. Ming-Sheng Zhang
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Correspondence to Yong-Hua Yang.

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Liu, Z., Qi, JL., Chen, L. et al. Effect of Light on Gene Expression and Shikonin Formation in Cultured Onosma Paniculatum Cells. Plant Cell Tiss Organ Cult 84, 38–48 (2006). https://doi.org/10.1007/s11240-005-8120-0

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  • DOI: https://doi.org/10.1007/s11240-005-8120-0

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