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Micronutrient optimization results into highly improved in vitro plant regeneration in kodo (Paspalum scrobiculatum L.) and finger (Eleusine coracana (L.) Gaertn.) millets

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Abstract

Basal medium constituents and their concentration play an important role in growth and morphogenesis of plant tissues cultured in vitro. In this study effect of different inorganic nutrients (CoCl2, MnSO4, ZnSO4, CuSO4 and AgNO3) on callus induction and plant regeneration in Paspalum scrobiculatum and Eleusine coracana was examined. A 5× and 3× increase in regeneration response at enhanced levels of CuSO4 was noted for kodo and finger millets, respectively. Significant improvement in plant regeneration was also observed with the increase in levels of Co and Mn. Addition of AgNO3 to the basal medium also had a stimulatory effect on callus induction and plant regeneration. Optimization of nutrient level in the basal medium has highly significant role in obtaining maximum regeneration response from explants and callus culture.

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Abbreviations

MS:

Murashige and Skoog (1962)

2,4-D:

2,4-Dichlorophenoxyacetic acid

Kn:

Kinetin

NAA:

α-Naphthaleneacetic acid

BAP:

6-Benzylaminopurine

GA3 :

Gibberellic acid

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Acknowledgements

Aditi Kothari-Chajer thanks CSIR; New Delhi for the award of Junior Research Fellowship and UGC for the award of commonwealth split site doctoral scholarship at Rothamsted Research Station, U.K. Manju Sharma thanks DST for the award of Women Scientist Scheme. The financial support provided by the UGC is also gratefully acknowledged.

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Authors and Affiliations

  1. Department of Botany, University of Rajasthan, Jaipur, India

    Aditi Kothari-Chajer, Manju Sharma, Sumita Kachhwaha & S. L. Kothari

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  1. Aditi Kothari-Chajer
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  2. Manju Sharma
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  3. Sumita Kachhwaha
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  4. S. L. Kothari
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Correspondence to S. L. Kothari.

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Kothari-Chajer, A., Sharma, M., Kachhwaha, S. et al. Micronutrient optimization results into highly improved in vitro plant regeneration in kodo (Paspalum scrobiculatum L.) and finger (Eleusine coracana (L.) Gaertn.) millets. Plant Cell Tiss Organ Cult 94, 105–112 (2008). https://doi.org/10.1007/s11240-008-9392-y

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  • DOI: https://doi.org/10.1007/s11240-008-9392-y

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