Abstract
The genetic fidelity of in vitro-raised gerbera clones was assessed by using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Out of 35 RAPD and 32 ISSR primers screened, only 12 RAPD and 10 ISSR primers produced clear, reproducible and scorable bands. The 12 RAPD primers produced 54 distinct and scorable bands, with an average of 4.5 bands per primer. The number of scorable bands for ISSR primers varied from 3 (ISSR-14) to 9 (ISSR-07), with an average of 5.5 bands per primer. The number of bands generated per primer was greater in ISSR than RAPD. All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant. A similarity matrix based on Jaccard’s coefficient revealed that the pair-wise value between the mother and the in vitro-raised plantlets was 1, indicating 100% similarity. This confirmed the true-to-type nature of the in vitro-raised clones.
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References
Bhatia R, Singh KP, Singh MC (2008) Effect of growth regulators on regeneration from leaf derived callus and shoot proliferation in gerbera. Indian J Hort 65:312–316
Devarumath RM, Nandy S, Rani V, Marimuthu S, Muraleedharan N (2002) RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C. assamica ssp. assamica (Assam-India type). Plant Cell Rep 21:166–173
Goto S, Thakur RC, Ishii K (1998) Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers. Plant Cell Rep 18:193–197
Joshi P, Dhawan V (2007) Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. Biol Plant 51:22–26
Kanwar JK, Kumar S (2008) In vitro propagation of Gerbera—a review. Hort Sci (Prague) 35:35–44
Lakshmanan V, Venkataramareddy SR, Neelwarne B (2007) Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers. Electron J Biotech 10:1–8
Martin M, Sarmento D, Oliveira MM (2004) Genetic stability of micropropagated almond plantlets, as assessed by RAPD and ISSR markers. Plant Cell Rep 23:492–496
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nuc Acids Res 8:4321–4325
Reynoird JP, Chriqui D, Noin M, Brown S, Marie D (1993) Plant regeneration from in vitro leaf culture of several Gerbera species. Plant Cell Tissue Organ Cult 33:203–210
Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket, New York
Salvi ND, George L, Eapen S (2001) Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tiss Organ Cult 66:113–119
Sreedhar RV, Venkatachalam L, Bhagyalakshmi N (2007) Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers. Biotechnol J 2:1007–1013
Varshney A, Lakshmikumaran M, Srivastava PS, Dhawan V (2001) Establishment of genetic fidelity of in vitro-raised Lilium bulblets through RAPD markers. In Vitro Cell Dev Biol Plant 37:227–231
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The fellowship provided by the Indian Agricultural Research Institute, New Delhi, to the senior author is gratefully acknowledged.
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Bhatia, R., Singh, K.P., Sharma, T.R. et al. Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell Tiss Organ Cult 104, 131–135 (2011). https://doi.org/10.1007/s11240-010-9806-5
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DOI: https://doi.org/10.1007/s11240-010-9806-5